FastQCFastQC Report
Fri 2 Aug 2019
HMM52AFXY_n01_1561.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHMM52AFXY_n01_1561.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences7852811
Sequences flagged as poor quality0
Sequence length76
%GC39

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACTGACCAATCTCGTATG846811.0783527070752117TruSeq Adapter, Index 4 (100% over 49bp)

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AAAAAGG190150.032.876170
CGTGTGC35200.032.2320861
GCTTGAA220200.030.28330260
TGCTTGA230750.028.61050859
CTGCTTG235050.028.04242958
ACGTGTG19200.026.9927121
GTGTGCT43000.026.214112
ATGCCGG14600.025.89202770
CTTGAAA261000.025.60268261
TCTGCTT282800.023.49321257
AAAAAAG274750.023.23726569
CTTCTGC293250.022.7036355
CCGTCTT296450.022.24332651
CGTCTTC301300.022.03656852
TTCTGCT305100.021.81049356
TTGAAAA309900.021.7659462
TGAAAAA314250.021.76539463
GTCTTCT310500.021.40617853
GCCGTCT312700.021.09860450
TCTTCTG326700.020.47549254
Produced by FastQC (version 0.11.5)