FastQCFastQC Report
Fri 2 Aug 2019
HMM52AFXY_n01_1560.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHMM52AFXY_n01_1560.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences7489671
Sequences flagged as poor quality0
Sequence length76
%GC38

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACTTAGGCATCTCGTATG2268913.029385402910221TruSeq Adapter, Index 3 (100% over 49bp)
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG118020.1575770150651477No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AAAAAGG346300.049.87810570
GCTTGAA445200.039.7428260
CTTGAAA461000.038.8742261
AAAAAAG457900.038.54778369
TGCTTGA475300.036.34226659
CGTGTGC61550.035.894941
TTGAAAA505400.035.708462
TGAAAAA529300.035.08795563
CTGCTTG498400.034.51038458
GAAAAAA564100.033.12787664
TCTGCTT544950.031.92859557
GTGTGCT72000.030.9213092
TTCTGCT558750.030.4945956
ACGTGTG30150.030.1938111
CTTCTGC583250.029.50769455
GTCTTCT611500.028.02852453
CGTCTTC626200.027.85104852
TCTTCTG616350.027.72995254
CCGTCTT640550.027.4183551
ATGCCGG38050.027.13580370
Produced by FastQC (version 0.11.5)