FastQCFastQC Report
Fri 2 Aug 2019
HMM52AFXY_n01_1559.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHMM52AFXY_n01_1559.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences10042074
Sequences flagged as poor quality0
Sequence length76
%GC38

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACCGATGTATCTCGTATG1818261.81064190524786TruSeq Adapter, Index 2 (100% over 49bp)

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AAAAAGG348400.039.98407470
CGTGTGC82400.035.6951641
GCTTGAA401800.034.6448460
CTGCTTG394800.034.15975658
TGCTTGA397400.034.1564659
GTGTGCT94200.031.1791692
TCTGCTT440450.031.00870157
CTTGAAA461850.030.59501861
ACGTGTG41050.029.7693651
CTTCTGC454700.029.5825955
CCGTCTT464650.029.43669751
AAAAAAG484950.029.3534669
CGTCTTC460900.029.34222652
TTCTGCT450200.029.34197456
GTCTTCT460200.028.88490953
GCCGTCT472550.028.61114550
TCTTCTG474950.027.96019654
TGCCGTC522050.026.71605949
ATGCCGG56950.026.67338270
ATGCCGT538300.025.99408548
Produced by FastQC (version 0.11.5)