FastQCFastQC Report
Fri 2 Aug 2019
HMM52AFXY_n01_1556.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHMM52AFXY_n01_1556.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences11136265
Sequences flagged as poor quality0
Sequence length76
%GC37

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACATCACGATCTCGTATG2814092.5269603408324066TruSeq Adapter, Index 1 (100% over 49bp)
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACAGATCGGAAGAGCACA163520.1468355862580497TruSeq Adapter, Index 14 (97% over 38bp)
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG127210.11423039951006912No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AAAAAGG450300.046.49904370
ATGCCGG29950.044.76117770
CGTGTGC121200.039.291951
GCTTGAA567300.039.07720660
TGCTTGA579550.038.03965859
CTGCTTG591050.037.20476558
CTTGAAA616700.036.13396561
ACGTGTG53850.035.308671
GTGTGCT136500.035.238392
AAAAAAG608100.035.00285369
TCTGCTT646450.034.0921757
CTTCTGC658750.033.44999755
TTGAAAA675850.033.24056662
TTCTGCT669350.032.85228756
CGTCTTC679050.032.44151752
TGAAAAA699500.032.19177263
GTCTTCT688100.032.00466553
TCTTCTG691700.031.82099754
CCGTCTT700350.031.48470151
GCCGTCG22000.029.59305470
Produced by FastQC (version 0.11.5)