FastQCFastQC Report
Fri 2 Aug 2019
HMM52AFXY_n01_1547.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHMM52AFXY_n01_1547.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences7492201
Sequences flagged as poor quality0
Sequence length76
%GC40

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACGTGAAAATCTCGTATG2142962.860254283087173TruSeq Adapter, Index 19 (97% over 49bp)
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG80580.10755183957291055No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AAAAAGG341700.047.2110270
GCTTGAA367800.044.11981260
TGCTTGA369300.042.89801859
CTGCTTG369650.042.46916258
CTTGAAA406500.040.33225661
TCTGCTT401500.039.4489157
CGTGTGC114850.039.149861
TTCTGCT403500.038.00395256
CTTCTGC414850.037.69824655
AAAAAAG453050.036.46559569
GTCTTCT422950.036.4446853
CGTCTTC434350.036.3664952
ACGTGTG50150.035.681751
CCGTCTT446950.035.6464551
TTGAAAA467750.035.2901362
TCTTCTG438250.035.2221454
GTGTGCT130250.034.8869252
TGAAAAA493050.034.7997163
GCCGTCT471250.033.44418750
ATGCCGG72150.033.4244770
Produced by FastQC (version 0.11.5)