FastQCFastQC Report
Fri 2 Aug 2019
HMM52AFXY_n01_1543.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHMM52AFXY_n01_1543.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences8555433
Sequences flagged as poor quality0
Sequence length76
%GC37

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACGTCCGCATCTCGTATG3709314.335619249195219TruSeq Adapter, Index 18 (100% over 49bp)
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG160990.18817282538475844No Hit
GATCGGAAGAGCACACGTCTGAACTCCAGTCACGTCCGCATCTCGTATGC105620.12345371648635434TruSeq Adapter, Index 18 (100% over 50bp)

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AAAAAGG532750.052.85379470
AAAAAAG672750.042.67737269
GCTTGAA687900.041.4091660
CGTGTGC147200.040.2001531
TGCTTGA695500.040.05556559
CTTGAAA720350.039.9665361
CTGCTTG703550.039.47287458
ACGTGTG59800.038.586291
TCTGCTT740100.037.97279757
TTGAAAA775750.037.3286962
TTCTGCT731700.037.29889756
CTTCTGC756100.036.8451955
TGAAAAA821450.036.4663863
GTGTGCT163400.036.353952
GTCTTCT767000.036.06306553
CGTCTTC787900.035.72834852
TCTTCTG775200.035.63032554
CCGTCTT809350.035.0190951
GAAAAAA860900.035.00676364
ATGCCGG73750.033.8851870
Produced by FastQC (version 0.11.5)