FastQCFastQC Report
Fri 2 Aug 2019
HMM52AFXY_n01_1215a.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHMM52AFXY_n01_1215a.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences9583762
Sequences flagged as poor quality0
Sequence length76
%GC39

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACCTTGTAATCTCGTATG4942835.157504954734894TruSeq Adapter, Index 12 (100% over 49bp)
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG158670.165561290023688No Hit
GATCGGAAGAGCACACGTCTGAACTCCAGTCACCTTGTAATCTCGTATGC148840.15530435751639074TruSeq Adapter, Index 12 (100% over 50bp)

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AAAAAGG667150.053.90552570
GCTTGAA743950.051.3120560
TGCTTGA765700.049.3971259
CTTGAAA782950.049.01463361
CTGCTTG774600.048.59910658
TCTGCTT826800.045.75517357
AAAAAAG808300.045.033569
TTGAAAA860450.044.72603662
TGAAAAA871900.044.6525363
TTCTGCT841900.044.53540456
CTTCTGC847950.044.41991455
GTCTTCT877700.042.82032853
CGTCTTC885650.042.7560652
CCGTCTT890250.042.68779851
GAAAAAA924050.042.37871664
TCTTCTG887150.042.3151254
GCCGTCT912050.041.5139650
TGCCGTC956450.039.999749
ATGCCGT983750.038.93947648
TATGCCG1019050.037.6964947
Produced by FastQC (version 0.11.5)