FastQCFastQC Report
Fri 2 Aug 2019
HMM52AFXY_n01_1214-2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHMM52AFXY_n01_1214-2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences13003847
Sequences flagged as poor quality0
Sequence length76
%GC40

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACTAGCTTATCTCGTATG5190523.9915265075019724TruSeq Adapter, Index 10 (100% over 49bp)
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACTAGCTTATCGCGTATG377680.2904371298739519TruSeq Adapter, Index 10 (97% over 49bp)
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG202920.1560461300413639No Hit
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACTAGCTTATCTCGTATT138840.10676840476514372TruSeq Adapter, Index 10 (97% over 48bp)

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AAAAAGG789050.054.25927770
GCTTGAA751550.052.78713660
TGCTTGA747700.051.22851659
CTGCTTG750050.050.6991558
CTTGAAA824050.048.7835661
TCTGCTT822800.047.0970557
TTCTGCT798900.045.62745356
AAAAAAG969650.045.08566769
CTTCTGC852100.044.82033555
TGAAAAA999500.044.11107363
TTGAAAA925350.044.0067362
GCCGTCT860600.043.6255950
CCGTCTT888350.043.37009451
GTCTTCT853200.043.0980553
CGTCTTC881150.043.01364552
TCTTCTG879200.041.90207754
GAAAAAA1076000.041.34251464
TGCCGTC961100.041.20488449
ATGCCGT971850.040.63745548
TATGCCG1048500.038.70073747
Produced by FastQC (version 0.11.5)