FastQCFastQC Report
Fri 2 Aug 2019
HMM52AFXY_n01_1204-2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHMM52AFXY_n01_1204-2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences6632847
Sequences flagged as poor quality0
Sequence length76
%GC40

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACGAGTGGATCTCGTATG3445875.195159785835554TruSeq Adapter, Index 7 (97% over 36bp)
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG105410.15892119929797868No Hit
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACGAGTGGATCGCGTATG95690.1442668585601326TruSeq Adapter, Index 7 (97% over 36bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACGAGTGGATCTCGTATGC75250.11345052886038227TruSeq Adapter, Index 7 (97% over 36bp)
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACGAGTGGAGCTCGTATG68900.10387696263761248TruSeq Adapter, Index 7 (97% over 36bp)

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AAAAAGG485000.054.5898370
GCTTGAA526100.051.37908660
TGCTTGA522400.050.67757459
CTGCTTG523500.050.33667858
CTTGAAA559150.048.79846261
TCTGCTT562100.047.2972457
AAAAAAG571450.046.99290569
CTTCTGC564700.046.74403855
TTCTGCT559800.046.37158256
CCGTCTT581400.046.0591251
CGTCTTC579300.045.86392252
GTCTTCT574550.045.44506553
GCCGTCT585800.045.37260450
TTGAAAA611250.044.97698262
TCTTCTG582150.044.8438154
TGAAAAA637600.044.53464563
TGCCGTC619950.043.82637849
ATGCCGT636900.042.81388548
GAAAAAA670000.042.51686564
TATGCCG665300.041.3535847
Produced by FastQC (version 0.11.5)