FastQCFastQC Report
Fri 2 Aug 2019
HMM52AFXY_n01_1091-2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHMM52AFXY_n01_1091-2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences5370004
Sequences flagged as poor quality0
Sequence length76
%GC40

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACGTGGCCATCTCGTATG2141573.9880231001690127TruSeq Adapter, Index 20 (97% over 49bp)
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG77430.144189836730103No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AAAAAGG310100.053.13745570
GCTTGAA338750.050.2992660
TGCTTGA348100.047.97287459
CTTGAAA359250.047.9644461
CTGCTTG355850.046.71168558
TTGAAAA389150.044.55795762
AAAAAAG377250.044.51445869
TGAAAAA405700.044.06027263
TCTGCTT393250.042.68750457
GAAAAAA433000.041.6780464
TTCTGCT394150.041.5328956
CTTCTGC404050.041.3468755
CCGTCTT426500.039.74270651
GTCTTCT417550.039.71482553
CGTCTTC425900.039.61827552
GCCGTCT430100.039.14151850
TCTTCTG423900.039.0639254
TGCCGTC459100.037.54541849
ATGCCGT472950.036.51992848
TATGCCG492150.035.38643647
Produced by FastQC (version 0.11.5)