FastQCFastQC Report
Thu 2 Nov 2023
HMLTGDMXY_n02_MTH-038.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHMLTGDMXY_n02_MTH-038.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences733304
Sequences flagged as poor quality0
Sequence length101
%GC39

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTTTCTTGTCAACTGTGATTGGACAAGAGTTAGCCGCTCCTAAGGGATAG55730.7599849448523395No Hit
CTCAAAAGGAAAATCCCTCGGTTTGTGCAAGCTTACCTTGGGAATTGTCA37030.5049747444443232No Hit
GTCATGTTGAGTAATTTTCCAATTAATGCCTTAGCATTCAAGCGAAGTGA27590.37624232242016953No Hit
GGAAAATCCCTCGGTTTGTGCAAGCTTACCTTGGGAATTGTCATGTTGAG25770.35142314783500433No Hit
CAAGAGTTAGCCGCTCCTAAGGGATAGCTGAAAAGTGTTTATTTGGGGAA24260.3308314150747848No Hit
GTCCTTGTTACTTTGAGTAAATTAGAGTGTTCAAAGCAAACAGATATAGC24040.32783129506998465No Hit
GTTTTCTTTGCACTTTACCTCTATAGCAAACCTTGGAATCAATTTACTTG22190.3026030132114375No Hit
GTTAAAACGCTCGTAGTTGAATTTCAAAGAATCGATATTTTAAGCAACGC21700.29592092774620077No Hit
GTTAAACATTTGGTACTTCGGTACCTTCTATACGCACAATTAATGTAATA21500.2931935459236551No Hit
GGTTTCTTGTCAACTGTGATTGGACAAGAGTTAGCCGCTCCTAAGGGATA21330.2908752713744914No Hit
CTGAAAAGTGTTTATTTGGGGAATGTTTTGTAAGAAATATGCATTTCATC20200.27546556407710854No Hit
GTTAAACATCTGGTACTTCGTTACCTTCTATACGCACAATTAATGTAATA19910.27151086043441736No Hit
GTTAGCCGCTCCTAAGGGATAGCTGAAAAGTGTTTATTTGGGGAATGTTT17650.24069144583965177No Hit
GTTGGTTCAGACAATTGGTACAGCAATTGGTTGACAAACCAGTGTTGCGA17630.2404187076573972No Hit
CTACGGAAAAGCTGTAGCTAATACTTGCTTTAGCACTCTTGATTCATTTC17250.2352366821945605No Hit
GTGAAAGTATATGCATATTTTATATGTAGAAACTGCGAACGGCTCATTAA17120.23346388400990584No Hit
GGGAAACAGGTTGATATTCCTGTGCCGATAGTATTTTGAGTGTAATAGAG15720.21437221125208644No Hit
GTTAAAACGCTCGTAGTTGAATTTCAAAGAATCGACATTTTAAGCAACGC14960.20400816032641306No Hit
GGTTGATATTCCTGTGCCGATAGTATTTTGAGTGTAATAGAGATGGTAAC14380.19609875304103072No Hit
ATTAAATAGTACCTGAAATCGTTAAGATGGAACGGATTAAGAGAGAAAAA14120.19255315667172143No Hit
CCTCGGTTTGTGCAAGCTTACCTTGGGAATTGTCATGTTGAGTAATTTTC13730.18723476211775744No Hit
GGGTTTCTTGTCAACTGTGATTGGACAAGAGTTAGCCGCTCCTAAGGGAT13720.18709839302663017No Hit
GAAAAGTGTTTATTTGGGGAATGTTTTGTAAGAAATATGCATTTCATCCC13460.18355279665732083No Hit
GAGAAATTATACGTGCTTCCTCTACGGATGCATTCATAATCCTATCACCT13300.18137089119928432No Hit
GTTGATATTCCTGTGCCGATAGTATTTTGAGTGTAATAGAGATGGTAACA13210.1801435693791388No Hit
GAGCCATTAAATAGTACCTGAAATCGTTAAGATGGAACGGATTAAGAGAG13160.1794617239235024No Hit
GCTGAAAAGTGTTTATTTGGGGAATGTTTTGTAAGAAATATGCATTTCAT12940.1764616039187022No Hit
GTTGAATTTCAAAGAATCGATATTTTAAGCAACGCTTCTAGCTTAATCCA12820.17482517482517482No Hit
ATTTACTTGGAGAAGAGGTTCGCTGAATCAATTCAGGCAAATTATTTTTT12380.16882493481557445No Hit
ATCTACTTGACATTTTTTCTATAAGGATAACTACGGAAAAGCTGTAGCTA12060.16446112389950143No Hit
GTGGAGAAGGGTTTCTTGTCAACTGTGATTGGACAAGAGTTAGCCGCTCC12040.16418838571724687No Hit
CTACTTGACATTTTTTCTATAAGGATAACTACGGAAAAGCTGTAGCTAAT11900.16227921844146495No Hit
CTTGTTAAGCCTTTTAAGAAAAAAGTTATTAACTTAAGGAATTATAACAA11530.15723356206975553No Hit
GACAATTGGTACAGCAATTGGTTGACAAACCAGTGTTGCGAAGCTAAGTC11250.15341522751819164No Hit
AGGGTTTCTTGTCAACTGTGATTGGACAAGAGTTAGCCGCTCCTAAGGGA11150.15205153660691884No Hit
CAATTTACTTGGAGAAGAGGTTCGCTGAATCAATTCAGGCAAATTATTTT11050.150687845695646No Hit
GGAAAAGCTGTAGCTAATACTTGCTTTAGCACTCTTGATTCATTTCTTGA11010.1501423693311369No Hit
GGATAACTACGGAAAAGCTGTAGCTAATACTTGCTTTAGCACTCTTGATT10950.1493241547843732No Hit
CTTGAGTGTGTACTTGTTAAGCCTTTTAAGAAAAAAGTTATTAACTTAAG10780.1470058802352094No Hit
AATCAATTTACTTGGAGAAGAGGTTCGCTGAATCAATTCAGGCAAATTAT10130.1381418893119361No Hit
GGCTGAACGCCTCTTAAGCCAGAAACCATGCTGATTAAACAATACTATCT9830.13405081657811768No Hit
GATTAGTTGGTTCAGACAATTGGTACAGCAATTGGTTGACAAACCAGTGT9820.13391444748699038No Hit
GCAAGCTTACCTTGGGAATTGTCATGTTGAGTAATTTTCCAATTAATGCC9580.13064158929993563No Hit
CATTTCTTGAGTGTGTACTTGTTAAGCCTTTTAAGAAAAAAGTTATTAAC9230.12586867111048078No Hit
CAGCGAACTTGGATAATTATGATAAAACAATTCACTGAAGTTGTTGGGAA8960.12218670565004419No Hit
CGGAAAAGCTGTAGCTAATACTTGCTTTAGCACTCTTGATTCATTTCTTG8940.12191396746778962No Hit
CTGAAATATGTTTTTACGACAGTGAAATACCACTACTTTTAAAGTTGTTT8930.12177759837666234No Hit
CTGAATTCTTAATTTCCTGTTTTGTAGTAATAGTAATTCGTTTTTATGAA8930.12177759837666234No Hit
GAGGATTAGTTGGTTCAGACAATTGGTACAGCAATTGGTTGACAAACCAG8930.12177759837666234No Hit
GAACTATCCTTGAAACAAGGAGGGAACAGCAATTAAAGGGGGATGATTTA8910.12150486019440777No Hit
GCCATTAAATAGTACCTGAAATCGTTAAGATGGAACGGATTAAGAGAGAA8790.1198684311008804No Hit
GCTAAGTCTGTTGGATAATGGCTGAACGCCTCTTAAGCCAGAAACCATGC8690.11850474018960759No Hit
GACAAGAGTTAGCCGCTCCTAAGGGATAGCTGAAAAGTGTTTATTTGGGG8680.1183683710984803No Hit
GTTGAATTTCAAAGAATCGACATTTTAAGCAACGCTTCTAGCTTAATCCA8500.11591372745818923No Hit
CAGTAATCCAACTTGGTACGAGAGGATTAGTTGGTTCAGACAATTGGTAC8460.11536825109368012No Hit
GATATGTTAAACATTTGGTACTTCGGTACCTTCTATACGCACAATTAATG8440.11509551291142554No Hit
AGCAAACCTTGGAATCAATTTACTTGGAGAAGAGGTTCGCTGAATCAATT8400.11455003654691642No Hit
CGAAAGGGAAACAGGTTGATATTCCTGTGCCGATAGTATTTTGAGTGTAA8380.11427729836466186No Hit
GTTGAGTAATTTTCCAATTAATGCCTTAGCATTCAAGCGAAGTGAGCAGG8380.11427729836466186No Hit
CTTGACATTTTTTCTATAAGGATAACTACGGAAAAGCTGTAGCTAATACT8240.11236813108887991No Hit
GTTTTGTAAGAAATATGCATTTCATCCCCGTCTCGAAAGGGAAACAGGTT8160.11127717835986166No Hit
GAGTTAGCCGCTCCTAAGGGATAGCTGAAAAGTGTTTATTTGGGGAATGT7970.10868616562844333No Hit
GTAAAAGAGAAATTATACGTGCTTCCTCTACGGATGCATTCATAATCCTA7870.10732247471717049No Hit
CTGAAATCGTTAAGATGGAACGGATTAAGAGAGAAAAAAAGTAAAGCGGG7840.10691336744378865No Hit
GGAGTCTAGCAAATGTGCAAGTGTGTATGATATGTTAAACATTTGGTACT7750.10568604562364312No Hit
CTTTACTCTAGTCTGGCTTTGTGAAACGACTTAAGAGGTGTAGCATAAGT7650.10432235471237031No Hit
CAAAAGGAAAATCCCTCGGTTTGTGCAAGCTTACCTTGGGAATTGTCATG7610.1037768783478612No Hit
GTTACTTTGAGTAAATTAGAGTGTTCAAAGCAAACAGATATAGCATTGCG7580.10336777107447935No Hit
GTTTTGTAAGAAATATGCATTTCATCCCCGTCTCAAAAGGGAAACAGGTT7580.10336777107447935No Hit
AGGGAAACAGGTTGATATTCCTGTGCCGATAGTATTTTGAGTGTAATAGA7530.10268592561884293No Hit
GGAGTCTAGCAAATGTGCAAGTGTGTATGATATGTTAAACATCTGGTACT7500.1022768183454611No Hit
GATATGTTAAACATCTGGTACTTCGTTACCTTCTATACGCACAATTAATG7500.1022768183454611No Hit
GGATTAGTTGGTTCAGACAATTGGTACAGCAATTGGTTGACAAACCAGTG7500.1022768183454611No Hit
CTTGGAATCAATTTACTTGGAGAAGAGGTTCGCTGAATCAATTCAGGCAA7480.10200408016320653No Hit
GTTTATTTGGGGAATGTTTTGTAAGAAATATGCATTTCATCCCCGTCTCG7460.10173134198095196No Hit
CAAGGACCAGCTCAAAAGGAAAATCCCTCGGTTTGTGCAAGCTTACCTTG7430.10132223470757011No Hit
GGAACATCAGCGAACTTGGATAATTATGATAAAACAATTCACTGAAGTTG7340.10009491288742459No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)