FastQCFastQC Report
Thu 2 Nov 2023
HMLTGDMXY_n02_MTH-037.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHMLTGDMXY_n02_MTH-037.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences8507576
Sequences flagged as poor quality0
Sequence length101
%GC38

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTTTCTTGTCAACTGTGATTGGACAAGAGTTAGCCGCTCCTAAGGGATAG651700.7660231304427959No Hit
CTCAAAAGGAAAATCCCTCGGTTTGTGCAAGCTTACCTTGGGAATTGTCA554470.6517367579202349No Hit
GTCATGTTGAGTAATTTTCCAATTAATGCCTTAGCATTCAAGCGAAGTGA404410.47535279144141646No Hit
GGAAAATCCCTCGGTTTGTGCAAGCTTACCTTGGGAATTGTCATGTTGAG383700.45100978233988154No Hit
GTTAAAACGCTCGTAGTTGAATTTCAAAGAATCGATATTTTAAGCAACGC359030.4220120983932439No Hit
GTTTTCTTTGCACTTTACCTCTATAGCAAACCTTGGAATCAATTTACTTG342230.4022649929897776No Hit
GTGAAAGTATATGCATATTTTATATGTAGAAACTGCGAACGGCTCATTAA309040.36325270558852485No Hit
GTTAAACATTTGGTACTTCGCTACCTTCTATACGCACAATTAATGTAATA285800.3359358764470632No Hit
CTACGGAAAAGCTGTAGCTAATACTTGCTTTAGCACTCTTGATTCATTTC281880.3313282185195877No Hit
CGAAAGGGAAACAGGTTGATATTCCTGTGCCGATAGTATTTTGAGTGTAA269990.3173524397548726No Hit
CAAGAGTTAGCCGCTCCTAAGGGATAGCTGAAAAGTGTTTATTTGGGGAA237960.2797036429648116No Hit
GGTTTCTTGTCAACTGTGATTGGACAAGAGTTAGCCGCTCCTAAGGGATA236800.27834015235361986No Hit
GTTGGTTCAGACAATTGGTACAGCAATTGGTTGACAAACCAGTGTTGCGA213540.25099981475334454No Hit
ATTTACTTGGAGAAGAGGTTCGCTGAATCAATTCAGGCAAATTATTTTTT211580.24869598578960683No Hit
AAACGACTTAAGAGGTGTAGCATAAGTGGGAGTAGAGACTGAAATATGTT203270.2389282211525351No Hit
CCTCGGTTTGTGCAAGCTTACCTTGGGAATTGTCATGTTGAGTAATTTTC201590.23695351061218844No Hit
GGATAACTACGGAAAAGCTGTAGCTAATACTTGCTTTAGCACTCTTGATT201490.2368359683181202No Hit
CTTGTTAAGCCTTTTAAGAAAAAAGTTATTAACTTAAGGAATTATAACAA200400.23555475731277625No Hit
ATTAAATAGTACCTGAAATCGTTAAGATGGAACGGATTAAGAGAGAAAAA198200.23296882684327472No Hit
GTTGAATTTCAAAGAATCGATATTTTAAGCAACGCTTCTAGCTTAATCCA196000.23038289637377318No Hit
GAGAAATTATACGTGCTTCCTCTACGGATGCATTCATAATCCTATCACCT191890.2255519080875681No Hit
GGAAAAGCTGTAGCTAATACTTGCTTTAGCACTCTTGATTCATTTCTTGA189700.22297773184747335No Hit
CTGAAATATGTTTTTACGACAGTGAAATACCACTACTTTTAAAGTTGTTT189050.22221370693602974No Hit
CAATTTACTTGGAGAAGAGGTTCGCTGAATCAATTCAGGCAAATTATTTT187410.2202860133133104No Hit
CTACTTGACATTTTTTCTATAAGGATAACTACGGAAAAGCTGTAGCTAAT181110.21288084878701052No Hit
GGTTGATATTCCTGTGCCGATAGTATTTTGAGTGTAATAGAGATGGTAAC180210.21182296814039628No Hit
ATCTACTTGACATTTTTTCTATAAGGATAACTACGGAAAAGCTGTAGCTA178520.20983650337064283No Hit
GTTTTGTAAGAAATATGCATTTCATCCCCGTCTCGAAAGGGAAACAGGTT175880.20673338680724096No Hit
CTTGAATTCTTAATTTCCTGTTTTGTAGTAATAGTAATTCGTTTTTATGA175440.20621620071334068No Hit
CTGAAAAGTGTTTATTTGGGGAATGTTTTGTAAGAAATATGCATTTCATC174600.20522884544316736No Hit
CTTGAGTGTGTACTTGTTAAGCCTTTTAAGAAAAAAGTTATTAACTTAAG173680.20414745633773942No Hit
GTTGATATTCCTGTGCCGATAGTATTTTGAGTGTAATAGAGATGGTAACA171260.20130293282128775No Hit
GGGAAACAGGTTGATATTCCTGTGCCGATAGTATTTTGAGTGTAATAGAG169250.19894033271051587No Hit
AATCAATTTACTTGGAGAAGAGGTTCGCTGAATCAATTCAGGCAAATTAT165870.194967403171009No Hit
GTTCTTGTTACTTTGAGTAAATTAGAGTGTTCAAAGCAAACAGATATAGC163010.19160569356065701No Hit
CATTTCTTGAGTGTGTACTTGTTAAGCCTTTTAAGAAAAAAGTTATTAAC158520.1863280445569925No Hit
GTAAGAAATATGCATTTCATCCCCGTCTCGAAAGGGAAACAGGTTGATAT158400.18618699380411058No Hit
GTTAGCCGCTCCTAAGGGATAGCTGAAAAGTGTTTATTTGGGGAATGTTT156980.18451789322834142No Hit
AGCAAACCTTGGAATCAATTTACTTGGAGAAGAGGTTCGCTGAATCAATT155040.18223757272341734No Hit
GTGGAGAAGGGTTTCTTGTCAACTGTGATTGGACAAGAGTTAGCCGCTCC154430.181520564729601No Hit
AACGACTTAAGAGGTGTAGCATAAGTGGGAGTAGAGACTGAAATATGTTT149590.17583151769669764No Hit
GTTTATTTGGGGAATGTTTTGTAAGAAATATGCATTTCATCCCCGTCTCG147230.17305751955668688No Hit
CGGAAAAGCTGTAGCTAATACTTGCTTTAGCACTCTTGATTCATTTCTTG144730.1701189622049806No Hit
CTTTACTCTAGTCTGGCTTTGTGAAACGACTTAAGAGGTGTAGCATAAGT144670.17004843682853965No Hit
GTTACTTTGAGTAAATTAGAGTGTTCAAAGCAAACAGATATAGCATTGCG143580.1687672258231957No Hit
GACAATTGGTACAGCAATTGGTTGACAAACCAGTGTTGCGAAGCTAAGTC141790.16666321875937398No Hit
GAACTATCCTTGAAACAAGGAGGGAACAGCAATTAAAGGGGGATGATTTA139920.1644651778602977No Hit
CTTGACATTTTTTCTATAAGGATAACTACGGAAAAGCTGTAGCTAATACT139390.16384220370173594No Hit
GAGCCATTAAATAGTACCTGAAATCGTTAAGATGGAACGGATTAAGAGAG139330.16377167832529502No Hit
GGGTTTCTTGTCAACTGTGATTGGACAAGAGTTAGCCGCTCCTAAGGGAT138850.1632074753137674No Hit
GGAGTCTAGCAAATGTGCAAGTGTGTATGAAATGTTAAACATTTGGTACT138510.16280783151393535No Hit
CAAGGACCAGCTCAAAAGGAAAATCCCTCGGTTTGTGCAAGCTTACCTTG137260.16133855283808218No Hit
GGAGTCTAGCAAATGTGCAAGTGTGTATGATATGTTAAACATTTGGTACT137100.161150485167573No Hit
CTTGGAATCAATTTACTTGGAGAAGAGGTTCGCTGAATCAATTCAGGCAA135600.15938735075654922No Hit
GGAACATCAGCGAACTTGGATAATTATGATAAAACAATTCACTGAAGTTG133500.15691896258111593No Hit
AGTAATTCGTTTTTATGAATTATCCGAAGCCGTAAGAACTATCCTTGAAA132380.15560248888755152No Hit
CGACTTAAGAGGTGTAGCATAAGTGGGAGTAGAGACTGAAATATGTTTTT127230.14954906074303656No Hit
CATAATCCTATCACCTTTTTCGTGAGCTGCTGTTCGTGAGCTGTGAGCTG126290.148444163178795No Hit
GTTGAGTAATTTTCCAATTAATGCCTTAGCATTCAAGCGAAGTGAGCAGG124670.14653997801488933No Hit
CAAACCTTGGAATCAATTTACTTGGAGAAGAGGTTCGCTGAATCAATTCA124610.14646945263844835No Hit
GTAAAAGAGAAATTATACGTGCTTCCTCTACGGATGCATTCATAATCCTA123820.14554086851530917No Hit
GACAAGAGTTAGCCGCTCCTAAGGGATAGCTGAAAAGTGTTTATTTGGGG117940.138629381624096No Hit
ATTTTTTTGGGGTTTCGGCCTTGAATTCTTAATTTCCTGTTTTGTAGTAA117630.1382650005124844No Hit
AGGGTTTCTTGTCAACTGTGATTGGACAAGAGTTAGCCGCTCCTAAGGGA117220.13778307710680457No Hit
GCCATTAAATAGTACCTGAAATCGTTAAGATGGAACGGATTAAGAGAGAA116390.13680747606603808No Hit
GCTAAGTCTGTTGGATAATGGCTGAACGCCTCTTAAGCCAGAAACCATGC116330.13673695068959713No Hit
AGAAATTATACGTGCTTCCTCTACGGATGCATTCATAATCCTATCACCTT115510.13577310387823746No Hit
CTGAAATCGTTAAGATGGAACGGATTAAGAGAGAAAAAAAGTAAAGCGGG114730.13485627398450512No Hit
ACAAGGACCAGCTCAAAAGGAAAATCCCTCGGTTTGTGCAAGCTTACCTT114330.1343861048082321No Hit
GATTAGTTGGTTCAGACAATTGGTACAGCAATTGGTTGACAAACCAGTGT113890.1338689187143318No Hit
GTTAAACATTTGGTACTTCGTTACCTTCTATACGCACAATTAATGTAATA113570.13349278337331338No Hit
CTTGATTCATTTCTTGAGTGTGTACTTGTTAAGCCTTTTAAGAAAAAAGT112240.13192947086220563No Hit
ATTCGGTTGATTTGCTTACTTTGAAGAAAATATTGGGAAACGTAACAGTT111920.13155333552118723No Hit
GAAACGACTTAAGAGGTGTAGCATAAGTGGGAGTAGAGACTGAAATATGT111820.13143579322711899No Hit
CTTGGAGAAGAGGTTCGCTGAATCAATTCAGGCAAATTATTTTTTTGGGG110840.1302838787452501No Hit
GGCTGAACGCCTCTTAAGCCAGAAACCATGCTGATTAAACAATACTATCT109780.12903793042812667No Hit
CAAGAGTTAGCCGCTCCTAAGGGATAGCTGAAAAGTGTTTATTTGGGGAG108720.12779198211100318No Hit
CAGTAATCCAACTTGGTACGAGAGGATTAGTTGGTTCAGACAATTGGTAC108280.12727479601710287No Hit
GAAAAGTGTTTATTTGGGGAATGTTTTGTAAGAAATATGCATTTCATCCC107370.12620516114108177No Hit
GAAATGTTAAACATTTGGTACTTCGCTACCTTCTATACGCATAATTAATG106100.124712374006415No Hit
CTTAAGAGGTGTAGCATAAGTGGGAGTAGAGACTGAAATATGTTTTTACG105100.12353695106573247No Hit
CAAAAGGAAAATCCCTCGGTTTGTGCAAGCTTACCTTGGGAATTGTCATG104890.12329011224818914No Hit
GTCCTTGTTACTTTGAGTAAATTAGAGTGTTCAAAGCAAACAGATATAGC104160.1224320535014909No Hit
GATTCTTCGGATTTATTTTTCTTCTTTATTAAAAGGAACATCAGCGAACT104050.12230275697801583No Hit
GAGGATTAGTTGGTTCAGACAATTGGTACAGCAATTGGTTGACAAACCAG102720.12073944446690807No Hit
GTTCGCTGAATCAATTCAGGCAAATTATTTTTTTGGGGTTTCGGCCTTGA102240.12017524145538049No Hit
CTGAATTCGGTTGATTTGCTTACTTTGAAGAAAATATTGGGAAACGTAAC99850.11736598062714926No Hit
GTTCAAAGCAAACAGATATAGCATTGCGCGTTTGAATACTACAGCATGGA99080.11646090496282373No Hit
GCAAGCTTACCTTGGGAATTGTCATGTTGAGTAATTTTCCAATTAATGCC97670.11480355861646138No Hit
CTTAATTTCCTGTTTTGTAGTAATAGTAATTCGTTTTTATGAATTATCCG97580.11469777055179994No Hit
AGAGGATTAGTTGGTTCAGACAATTGGTACAGCAATTGGTTGACAAACCA97430.11452145711069758No Hit
CAGCGAACTTGGATAATTATGATAAAACAATTCACTGAAGTTGTTTGGAA92410.10862083394847133No Hit
GGATTAGTTGGTTCAGACAATTGGTACAGCAATTGGTTGACAAACCAGTG92010.10815066477219834No Hit
GAAGGGTTTCTTGTCAACTGTGATTGGACAAGAGTTAGCCGCTCCTAAGG90520.10639928459058139No Hit
GCTGAAAAGTGTTTATTTGGGGAATGTTTTGTAAGAAATATGCATTTCAT90020.10581157312024013No Hit
AGGGAAACAGGTTGATATTCCTGTGCCGATAGTATTTTGAGTGTAATAGA89010.10462439595015079No Hit
GTTTGACTGGGGCGGTACATCTGTTAAACAATAACGCAGATGTCCAAAGA87970.10340195609184098No Hit
CTTGTTACTTTGAGTAAATTAGAGTGTTCAAAGCAAACAGATATAGCATT86910.10215600777471749No Hit
CTGAAAAGTGTTTATTTGGGGAGTGTTTTGTAAGAAATATGCATTTCATC86440.10160355899259672No Hit
ACTTGACATTTTTTCTATAAGGATAACTACGGAAAAGCTGTAGCTAATAC85570.10058094103420293No Hit
CCATTAAATAGTACCTGAAATCGTTAAGATGGAACGGATTAAGAGAGAAA85350.10032234798725277No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)