FastQCFastQC Report
Thu 2 Nov 2023
HMLTGDMXY_n02_MTH-033.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHMLTGDMXY_n02_MTH-033.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences9252900
Sequences flagged as poor quality0
Sequence length101
%GC38

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTCAAAAGGAAAATCCCTCGGTTTGTGCAAGCTTACCTTGGGAATTGTCA751130.8117779290816933No Hit
GTGAAAGTATATGCATATTTTATATGTAGAAACTGCGAACGGCTCATTAA459190.4962660355131905No Hit
GTCATGTTGAGTAATTTTCCAATTAATGCCTTAGCATTCAAGCGAAGTGA441750.477417890607269No Hit
GGAAAATCCCTCGGTTTGTGCAAGCTTACCTTGGGAATTGTCATGTTGAG416540.4501723783894779No Hit
GTTTCTTGTCAACTGTGATTGGACAAGAGTTAGCCGCTCCTAAGGGATAG411860.44511450464178803No Hit
AGAAGACCCTGTTGAGCTTTACTCTAGTCTGGCTTTGTGAAACGACTTAA354970.38363107782424966No Hit
GTTTTCTTTGCACTTTACCTCTATAGCAAACCTTGGAATCAATTTACTTG349410.3776221508932335No Hit
AAACGACTTAAGAGGTGTAGCATAAGTGGGAGTAGAGACTGAAATATGTT299270.3234337342887095No Hit
GTTAAACATTTGGTACTTCGCTACCTTCTATACGCACAATTAATGTAATA298240.3223205697673162No Hit
CGAAAGGGAAACAGGTTGATATTCCTGTGCCGATAGTATTTTGAGTGTAA282190.30497465659414885No Hit
CTGAAATATGTTTTTACGACAGTGAAATACCACTACTTTTAAAGTTGTTT280370.3030077056922694No Hit
CTTGTTAAGCCTTTTAAGAAAAAAGTTATTAACTTAAGGAATTATAACAA229530.24806276951009953No Hit
CTTTACTCTAGTCTGGCTTTGTGAAACGACTTAAGAGGTGTAGCATAAGT229240.24774935425650335No Hit
CCTCGGTTTGTGCAAGCTTACCTTGGGAATTGTCATGTTGAGTAATTTTC228970.24745755384798282No Hit
GGACATTAGAATAGAGAAGAAAAGAAAATGGTTGAAAATAAAGGTATCGA222490.24045434404348906No Hit
CCCAATTCTAAAGAAGAGAGGTAGTGACAAGAAATAACAATACAAGGCCA218340.23596926369030252No Hit
CTACGGAAAAGCTGTAGCTAATACTTGCTTTAGCACTCTTGATTCATTTC214780.23212182126684608No Hit
CAAGAGTTAGCCGCTCCTAAGGGATAGCTGAAAAGTGTTTATTTGGGGAA213270.23048990046363843No Hit
ATTTACTTGGAGAAGAGGTTCGCTGAATCAATTCAGGCAAATTATTTTTT209860.226804569378249No Hit
GTCCTTGTTACTTTGAGTAAATTAGAGTGTTCAAAGCAAACAGATATAGC202130.21845043175653037No Hit
AGCAAACCTTGGAATCAATTTACTTGGAGAAGAGGTTCGCTGAATCAATT200160.216321369516584No Hit
GTTAAAACGCTCGTAGTTGAATTTCAAAGAATCGACATTTTAAGCAACGC193810.20945865620508164No Hit
GTTTGACTGGGGCGGTACATCTGTTAAACAATAACGCAGATGTCCAAAGA185880.2008883701326071No Hit
GTAACAACTCACCTACCGAATGAACTAGCCCTGAAAATGGATGGCGCTAA180950.19556031082147216No Hit
CAATTTACTTGGAGAAGAGGTTCGCTGAATCAATTCAGGCAAATTATTTT179690.19419857558170953No Hit
AATCAATTTACTTGGAGAAGAGGTTCGCTGAATCAATTCAGGCAAATTAT175090.189227161214322No Hit
CATAATCCTATCACCTTTTTCGTGAGCTGCTGTTCGTGAGCTGTGAGCTG173500.18750878103081195No Hit
GTTTTGTAAGAAATATGCATTTCATCCCCGTCTCGAAAGGGAAACAGGTT170910.18470965859352204No Hit
CGGGTATTCATATTAATTGAATTAATAAGTGGCACTTCTCATAGTGTTTC167560.1810891720433594No Hit
CTGAAAAGTGTTTATTTGGGGAATGTTTTGTAAGAAATATGCATTTCATC165500.17886284300057279No Hit
CAAAAGGAAAATCCCTCGGTTTGTGCAAGCTTACCTTGGGAATTGTCATG162970.17612856509850966No Hit
AACGACTTAAGAGGTGTAGCATAAGTGGGAGTAGAGACTGAAATATGTTT161450.1744858368727642No Hit
GTTGAATTTCAAAGAATCGACATTTTAAGCAACGCTTCTAGCTTAATCCA160920.17391304347826086No Hit
GGATAACTACGGAAAAGCTGTAGCTAATACTTGCTTTAGCACTCTTGATT157390.17009802332241783No Hit
CTTGGAATCAATTTACTTGGAGAAGAGGTTCGCTGAATCAATTCAGGCAA156610.1692550443644695No Hit
GTAAGAAATATGCATTTCATCCCCGTCTCGAAAGGGAAACAGGTTGATAT153890.1663154254341882No Hit
GTAAAAGAGAAATTATACGTGCTTCCTCTACGGATGCATTCATAATCCTA151980.16425120772946858No Hit
CAAGGACCAGCTCAAAAGGAAAATCCCTCGGTTTGTGCAAGCTTACCTTG151390.16361356979973846No Hit
AGAAAAGAAAATGGTTGAAAATAAAGGTATCGATGTATCCTTAGATTTCT150980.1631704654756887No Hit
GAAGACCCTGTTGAGCTTTACTCTAGTCTGGCTTTGTGAAACGACTTAAG148770.16078202509483513No Hit
GGTTTCTTGTCAACTGTGATTGGACAAGAGTTAGCCGCTCCTAAGGGATA145390.15712911627705908No Hit
GAAACGACTTAAGAGGTGTAGCATAAGTGGGAGTAGAGACTGAAATATGT145190.1569129678263031No Hit
GTTAGCCGCTCCTAAGGGATAGCTGAAAAGTGTTTATTTGGGGAATGTTT143060.15461098682575192No Hit
GTTTATTTGGGGAATGTTTTGTAAGAAATATGCATTTCATCCCCGTCTCG142430.15393011920587057No Hit
GAACTATCCTTGAAACAAGGAGGGAACAGCAATTAAAGGGGGATGATTTA142280.15376800786780362No Hit
GAAGAAAAGAAAATGGTTGAAAATAAAGGTATCGATGTATCCTTAGATTT138700.14989895059927158No Hit
CATTTCTTGAGTGTGTACTTGTTAAGCCTTTTAAGAAAAAAGTTATTAAC137530.1486344821623491No Hit
CGGAAAAGCTGTAGCTAATACTTGCTTTAGCACTCTTGATTCATTTCTTG137520.1486236747398113No Hit
GGAAAAGCTGTAGCTAATACTTGCTTTAGCACTCTTGATTCATTTCTTGA137030.14809411103545916No Hit
CTTTGTGAAACGACTTAAGAGGTGTAGCATAAGTGGGAGTAGAGACTGAA133130.14387921624571756No Hit
ATCTACTTGACATTTTTTCTATAAGGATAACTACGGAAAAGCTGTAGCTA132440.14313350409060943No Hit
CTACTTGACATTTTTTCTATAAGGATAACTACGGAAAAGCTGTAGCTAAT132430.14312269666807162No Hit
GAAATATATTACTCGGTTCGCCGGGTATTCATATTAATTGAATTAATAAG132160.14283089625955106No Hit
AGCATAAGTGGGAGTAGAGACTGAAATATGTTTTTACGACAGTGAAATAC131560.1421824509072831No Hit
GGAGTCTAGCAAATGTGCAAGTGTGTATGATATGTTAAACATTTGGTACT129850.14033438165331952No Hit
GACAAGAGTTAGCCGCTCCTAAGGGATAGCTGAAAAGTGTTTATTTGGGG129760.1402371148504793No Hit
CTTGAGTGTGTACTTGTTAAGCCTTTTAAGAAAAAAGTTATTAACTTAAG129370.13981562537150516No Hit
GTTGAGTAATTTTCCAATTAATGCCTTAGCATTCAAGCGAAGTGAGCAGG128000.13833500848382668No Hit
GAGAAATTATACGTGCTTCCTCTACGGATGCATTCATAATCCTATCACCT127760.1380756303429195No Hit
CAAACCTTGGAATCAATTTACTTGGAGAAGAGGTTCGCTGAATCAATTCA127130.1373947627230382No Hit
CTTGATTCATTTCTTGAGTGTGTACTTGTTAAGCCTTTTAAGAAAAAAGT126920.13716780684974442No Hit
ATTCGGTTGATTTGCTTACTTTGAAGAAAATATTGGGAAACGTAACAGTT126200.13638967242702288No Hit
GTTACTTTGAGTAAATTAGAGTGTTCAAAGCAAACAGATATAGCATTGCG124310.13434706956737888No Hit
AGAGAAGAAAAGAAAATGGTTGAAAATAAAGGTATCGATGTATCCTTAGA124080.1340984988490095No Hit
CGACTTAAGAGGTGTAGCATAAGTGGGAGTAGAGACTGAAATATGTTTTT123460.13342843865166595No Hit
ATTCAAGCGAAGTGAGCAGGAAAGCTCTCCCTAAGAGGGTGAAAGGCCCG122050.1319045920738363No Hit
GGGAAATCGTTTTCCTTGACCTATTCTCAAACTCCCAATAGGTAAAAAGG121420.13122372445395497No Hit
ATTAGAATAGAGAAGAAAAGAAAATGGTTGAAAATAAAGGTATCGATGTA120250.1299592560170325No Hit
ATTAAATAGTACCTGAAATCGTTAAGATGGAACGGATTAAGAGAGAAAAA118710.12829491294621145No Hit
GTACTTGTTAAGCCTTTTAAGAAAAAAGTTATTAACTTAAGGAATTATAA118580.12815441645322007No Hit
CTTAAGAGGTGTAGCATAAGTGGGAGTAGAGACTGAAATATGTTTTTACG116870.12630634719925643No Hit
AGTAATTCGTTTTTATGAATTATCCGAAGCCGTAAGAACTATCCTTGAAA115990.12535529401593012No Hit
TGTGAAACGACTTAAGAGGTGTAGCATAAGTGGGAGTAGAGACTGAAATA114970.12425293691707465No Hit
GTGGAGAAGGGTTTCTTGTCAACTGTGATTGGACAAGAGTTAGCCGCTCC113300.12244809735326222No Hit
GGAGTCTAGCAAATGTGCAAGTGTGTATGAAATGTTAAACATTTGGTACT113110.12224275632504403No Hit
CTGGAGTTGACTAATATAATTTTACCAGGTAGAGATAATGATTAGAGGAA112540.1216267332403895No Hit
GCAAGCTTACCTTGGGAATTGTCATGTTGAGTAATTTTCCAATTAATGCC111580.12058922067676081No Hit
GTTGTTTTACTTATCTATTGATGGGGAAATATATTACTCGGTTCGCCGGG111230.12021096088793784No Hit
TATTACTCGGTTCGCCGGGTATTCATATTAATTGAATTAATAAGTGGCAC111030.11999481243718185No Hit
GTTTAAGGCAACAACAGGTCTGTGATGTCCTTAGATGAACTAGGCTGCAC110120.11901133698624215No Hit
CGCACAATTAATGTAATATGTTAATTATTGTAGAACTGGCTGGAAGGCAA108130.11686065990122016No Hit
GAACAAGGACCAGCTCAAAAGGAAAATCCCTCGGTTTGTGCAAGCTTACC107390.11606091063342304No Hit
CGGCGAGTGAACAAGGACCAGCTCAAAAGGAAAATCCCTCGGTTTGTGCA107310.11597445125312064No Hit
CTTAATTTCCTGTTTTGTAGTAATAGTAATTCGTTTTTATGAATTATCCG107080.11572588053475126No Hit
GTTTTACTTATCTATTGATGGGGAAATATATTACTCGGTTCGCCGGGTAT107050.11569345826713787No Hit
GTTCTTTTAATTAAAATGATTCTTTTTAAGGACTTTCTTTGCTTCGGCTT106130.11469917539366037No Hit
GTGAACAAGGACCAGCTCAAAAGGAAAATCCCTCGGTTTGTGCAAGCTTA104130.11253769088610058No Hit
GTTTCTTTTTCGCGTAAGAATGTATTTGCTTGATTGTAAAGCTTCTTAGA103300.11164067481546326No Hit
GGGAAATATATTACTCGGTTCGCCGGGTATTCATATTAATTGAATTAATA103220.11155421543516088No Hit
ATTGGCTCTGAGGACATTAGAATAGAGAAGAAAAGAAAATGGTTGAAAAT103060.11138129667455608No Hit
GTGAAATACCACTACTTTTAAAGTTGTTTTACTTATCTATTGATGGGGAA102300.11055993256168337No Hit
CTCTAGTCTGGCTTTGTGAAACGACTTAAGAGGTGTAGCATAAGTGGGAG101890.11011682823763361No Hit
GAAAGATCTCTCAGGATCGCTGGAGTTGACTAATATAATTTTACCAGGTA101770.10998713916718002No Hit
GCTCATTAAAACAGTTATAATCTACTTGACATTTTTTCTATAAGGATAAC99780.10783646208215802No Hit
GAAAGTATATGCATATTTTATATGTAGAAACTGCGAACGGCTCATTAAAA99470.10750143198348626No Hit
GAAAAGAAAATGGTTGAAAATAAAGGTATCGATGTATCCTTAGATTTCTA98560.10651795653254656No Hit
GTTCAAAGCAAACAGATATAGCATTGCGCGTTTGAATACTACAGCATGGA97620.10550205881399347No Hit
GTTGGTTCAGACAATTGGTACAGCAATTGGTTGACAAACCAGTGTTGCGA97580.10545882912384225No Hit
CTTGACATTTTTTCTATAAGGATAACTACGGAAAAGCTGTAGCTAATACT97520.10539398458861546No Hit
CTGAATTCGGTTGATTTGCTTACTTTGAAGAAAATATTGGGAAACGTAAC97480.10535075489846427No Hit
ACAAGGACCAGCTCAAAAGGAAAATCCCTCGGTTTGTGCAAGCTTACCTT97010.10484280603918772No Hit
GTAATATGTTAATTATTGTAGAACTGGCTGGAAGGCAACTTCCTGTCGGC95650.10337299657404705No Hit
CTCGGTTTGTGCAAGCTTACCTTGGGAATTGTCATGTTGAGTAATTTTCC95330.10302715905283748No Hit
GTAAGAACTATCCTTGAAACAAGGAGGGAACAGCAATTAAAGGGGGATGA94220.10182753515114179No Hit
GGAAAGCTCTCCCTAAGAGGGTGAAAGGCCCGTAGTTTGTTGCTTTCTAT93680.10124393433410066No Hit
CAGCGAACTTGGATAATTATGATAAAACAATTCACTGAAGTTGTTGGGAA93320.10085486712273989No Hit
TGAAAGTATATGCATATTTTATATGTAGAAACTGCGAACGGCTCATTAAA92540.10001188816479159No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)