FastQCFastQC Report
Thu 2 Nov 2023
HMLTGDMXY_n02_MTH-021.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHMLTGDMXY_n02_MTH-021.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences69887470
Sequences flagged as poor quality0
Sequence length101
%GC40

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTTTCTTGTCAACTGTGATTGGACAAGAGTTAGCCGCTCCTAAGGGATAG3953010.5656249968699683No Hit
GGAAAATCCCTCGGTTTGTGCAAGCTTACCTTGGGAATTGTCATGTTGAG3850520.5509599932577327No Hit
CTCAAAAGGAAAATCCCTCGGTTTGTGCAAGCTTACCTTGGGAATTGTCA2833430.40542746789946754No Hit
GTTTTCTTTGCACTTTACCTCTATAGCAAACCTTGGAATCAATTTACTTG2225090.31838182151965155No Hit
GTTAGCCGCTCCTAAGGGATAGCTGAAAAGTGTTTATTTGGGGAATGTTT2163890.309624886979025No Hit
CTTGTCAACTGTGATTGGACAAGAGTTAGCCGCTCCTAAGGGATAGCTGA1957940.2801560852038284No Hit
TTTCTTGTCAACTGTGATTGGACAAGAGTTAGCCGCTCCTAAGGGATAGC1950930.27915304417229586No Hit
GCAAGCTTACCTTGGGAATTGTCATGTTGAGTAATTTTCCAATTAATGCC1788240.2558741931851303No Hit
CTTGGAGAAGAGGTTCGCTGAATCAATTCAGGCAAATTATTTTTTTGGGG1734160.24813603926426295No Hit
CAAGAGTTAGCCGCTCCTAAGGGATAGCTGAAAAGTGTTTATTTGGGGAA1707370.24430273409525338No Hit
CTTTACTCTAGTCTGGCTTTGTGAAACGACTTAAGAGGTGTAGCATAAGT1651660.23633134809430073No Hit
CGGAAAAGCTGTAGCTAATACTTGCTTTAGCACTCTTGATTCATTTCTTG1640280.23470301614867445No Hit
CTTGGAATCAATTTACTTGGAGAAGAGGTTCGCTGAATCAATTCAGGCAA1542880.22076632620983416No Hit
AGCAAACCTTGGAATCAATTTACTTGGAGAAGAGGTTCGCTGAATCAATT1521690.2177343091687251No Hit
CCTCGGTTTGTGCAAGCTTACCTTGGGAATTGTCATGTTGAGTAATTTTC1498050.21435172857165954No Hit
GTCCTTGTTACTTTGAGTAAATTAGAGTGTTCAAAGCAAACAGATATAGC1379430.1973787289767393No Hit
GTCATGTTGAGTAATTTTCCAATTAATGCCTTAGCATTCAAGCGAAGTGA1354110.19375576194130364No Hit
GTGCAAGTGTGTATGATATGTTAAACATTTGGTACTTCGTTACCTTCTAT1315470.18822687385879042No Hit
GGAAAAGCTGTAGCTAATACTTGCTTTAGCACTCTTGATTCATTTCTTGA1297440.1856470122612823No Hit
GTTCAAAGCAAACAGATATAGCATTGCGCGTTTGAATACTACAGCATGGA1296230.18547387679078953No Hit
GGGAAATCGTTTTCCTTGACCTATTCTCAAACTCCCAATAGGTAAAAAGG1265730.1811097182370459No Hit
GTTCGCTGAATCAATTCAGGCAAATTATTTTTTTGGGGTTTCGGCCTTGA1238730.1772463647632401No Hit
ATTTACTTGGAGAAGAGGTTCGCTGAATCAATTCAGGCAAATTATTTTTT1229930.17598719770511081No Hit
CGCTGAATCAATTCAGGCAAATTATTTTTTTGGGGTTTCGGCCTTGAATT1154200.16515120664691396No Hit
CTTGTTAAGCCTTTTAAGAAAAAAGTTATTAACTTAAGGAATTATAACAA1129360.16159692145101262No Hit
GGAGTCTAGCAAATGTGCAAGTGTGTATGATATGTTAAACATTTGGTACT1116330.15973249568198705No Hit
TTCTTGTCAACTGTGATTGGACAAGAGTTAGCCGCTCCTAAGGGATAGCT1113280.1592960798266127No Hit
GTTGGTTCAGACAATTGGTACAGCAATTGGTTGACAAACCAGTGTTGCGA1076330.1540090090541266No Hit
GAGAAATTATACGTGCTTCCTCTACGGATGCATTCATAATCCTATCACCT1070690.15320199743959825No Hit
GTTACTTTGAGTAAATTAGAGTGTTCAAAGCAAACAGATATAGCATTGCG1048930.15008842071404216No Hit
CTTGACATTTTTTCTATAAGGATAACTACGGAAAAGCTGTAGCTAATACT1040300.14885357847408126No Hit
GAACTAGGCTGCACGCGTGCTACACTGATATGTATAACGAGTTATTAAAA1031730.14762732146406216No Hit
CTAGGCTGCACGCGTGCTACACTGATATGTATAACGAGTTATTAAAATTA1028650.14718661299371688No Hit
GAAAATCCCTCGGTTTGTGCAAGCTTACCTTGGGAATTGTCATGTTGAGT1013780.14505890683980976No Hit
GGGAAACAGGTTGATATTCCTGTGCCGATAGTATTTTGAGTGTAATAGAG1006010.14394711956234787No Hit
CTAGCAAATGTGCAAGTGTGTATGATATGTTAAACATTTGGTACTTCGTT988260.14140732237123477No Hit
CATAATCCTATCACCTTTTTCGTGAGCTGCTGTTCGTGAGCTGTGAGCTG986120.14110111583664425No Hit
ATTTTTTTGGGGTTTCGGCCTTGAATTCTTAATTTCCTGTTTTGTAGTAA961180.13753252192417326No Hit
GTCTAGCAAATGTGCAAGTGTGTATGATATGTTAAACATTTGGTACTTCG959080.13723203887621055No Hit
TATTACTCGGTTCGCCGGGTATTCATATTAATTGAATTAATAAGTGGCAC946830.1354792210964283No Hit
GTGAAAGTATATGCATATTTTATATGTAGAAACTGCGAACGGCTCATTAA942390.1348439140807358No Hit
CAAAAGGAAAATCCCTCGGTTTGTGCAAGCTTACCTTGGGAATTGTCATG941960.13478238659948627No Hit
GTTAAACATTTGGTACTTCGTTACCTTCTATACGCACAATTAATGTAATA939440.13442180694193107No Hit
GGTTTCTTGTCAACTGTGATTGGACAAGAGTTAGCCGCTCCTAAGGGATA915930.13105782767640609No Hit
CTACTTGACATTTTTTCTATAAGGATAACTACGGAAAAGCTGTAGCTAAT915840.1310449498314934No Hit
ATTAAATAGTACCTGAAATCGTTAAGATGGAACGGATTAAGAGAGAAAAA909630.13015637853251807No Hit
CTGAAATATGTTTTTACGACAGTGAAATACCACTACTTTTAAAGTTGTTT907280.12982012369313126No Hit
GGCAAATTATTTTTTTGGGGTTTCGGCCTTGAATTCTTAATTTCCTGTTT897630.12843933254415993No Hit
CGGCGAGTGAACAAGGACCAGCTCAAAAGGAAAATCCCTCGGTTTGTGCA880480.12598538765246473No Hit
GAGCCATTAAATAGTACCTGAAATCGTTAAGATGGAACGGATTAAGAGAG871520.12470332664782399No Hit
CTCGGTTTGTGCAAGCTTACCTTGGGAATTGTCATGTTGAGTAATTTTCC864770.12373748827937253No Hit
CTTGGAAGTCCTTGTTACTTTGAGTAAATTAGAGTGTTCAAAGCAAACAG833710.11929320091283889No Hit
GTTGAGTAATTTTCCAATTAATGCCTTAGCATTCAAGCGAAGTGAGCAGG803390.11495479804892064No Hit
GTGCAAGTGTGTATGAAATGTTAAACATTTGGTACTTCGCTACCTTCTAT801770.1147229968404923No Hit
GAAAGATCTCTCAGGATCGCTGGAGTTGACTAATATAATTTTACCAGGTA777560.11125885655897975No Hit
CAGCGAACTTGGATAATTATGATAAAACAATTCACTGAAGTTGTTGGGAA770030.110181410201285No Hit
GGAGAAGGGTTTCTTGTCAACTGTGATTGGACAAGAGTTAGCCGCTCCTA762110.10904815984896864No Hit
GTTAAACATTTGGTACTTCGCTACCTTCTATACGCACAATTAATGTAATA756860.10829695222906194No Hit
GTTTGACTGGGGCGGTACATCTGTTAAACAATAACGCAGATGTCCAAAGA752500.10767309218662517No Hit
GCCATTAAATAGTACCTGAAATCGTTAAGATGGAACGGATTAAGAGAGAA750890.10744272184985378No Hit
TGTGAAACGACTTAAGAGGTGTAGCATAAGTGGGAGTAGAGACTGAAATA750720.10741839703168536No Hit
TTTTGAGTGTAATAGAGATGGTAACATATATACAAATGAACTCCTTCACA744980.10659707670058738No Hit
CAAGGACCAGCTCAAAAGGAAAATCCCTCGGTTTGTGCAAGCTTACCTTG737490.10552535382952052No Hit
GGTTGATATTCCTGTGCCGATAGTATTTTGAGTGTAATAGAGATGGTAAC728700.10426761764304818No Hit
AAACGACTTAAGAGGTGTAGCATAAGTGGGAGTAGAGACTGAAATATGTT721160.10318874041369648No Hit
GTTAAAACGCTCGTAGTTGAATTTCAAAGAATCGATATTTTAAGCAACGC715240.10234166439277313No Hit
AATCAATTTACTTGGAGAAGAGGTTCGCTGAATCAATTCAGGCAAATTAT714600.10225008860672735No Hit
CGACTTAAGAGGTGTAGCATAAGTGGGAGTAGAGACTGAAATATGTTTTT713350.1020712296496067No Hit
CGCACAATTAATGTAATATGTTAATTATTGTAGAACTGGCTGGAAGGCAA710680.10168918691719703No Hit
CAGTAATCCAACTTGGTACGAGAGGATTAGTTGGTTCAGACAATTGGTAC710610.10167917081559828No Hit
GTAAGAAATATGCATTTCATCCCCGTCTCGAAAGGGAAACAGGTTGATAT702290.10048868559700329No Hit
AGAAATTATACGTGCTTCCTCTACGGATGCATTCATAATCCTATCACCTT701880.10043001985906772No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)