FastQCFastQC Report
Thu 2 Nov 2023
HMLTGDMXY_n02_MTH-005.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHMLTGDMXY_n02_MTH-005.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2909122
Sequences flagged as poor quality0
Sequence length101
%GC37

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTTTCTTGTCAACTGTGATTGGACAAGAGTTAGCCGCTCCTAAGGGATAG267930.9209995318175037No Hit
CTCAAAAGGAAAATCCCTTGGTTTGTGCAAGCTTACCTTGGGAATTGTCA189480.6513305389048655No Hit
GTCATGTTGAGTAATTTTCCAATTAATGCCTTAGCATTCAAGCGAAGTGA177610.6105278499836033No Hit
CTCAAAAGGAAAATCCCTCGGTTTGTGCAAGCTTACCTTGGGAATTGTCA177370.6097028588006966No Hit
GTTTTCTTTGCACTTTACCTCTATAGCAAACCTTGGAATCAATTTACTTG160440.5515066057731508No Hit
GTGAAAGTATATGCATATTTTATATGTAGAAACTGCGAACGGCTCATTAA125000.4296829077639233No Hit
GTTAAACATTTGGTACTTCGTTACCTTCTATACGCACAATTAATGTAATA120550.4143861962475276No Hit
CAAGAGTTAGCCGCTCCTAAGGGATAGCTGAAAAGTGTTTATTTGGGGAA115110.39568639610164164No Hit
GTTAAACATTTGGTACTTCGCTACCTTCTATACGCACAATTAATGTAATA115030.39541139904067274No Hit
GTCCTTGTTACTTTGAGTAAATTAGAGTGTTCAAAGCAAACAGATATAGC111510.38331152835804067No Hit
ATTTACTTGGAGAAGAGGTTCGCTGAATCAATTCAGGCAAATTATTTTTT105490.36261799952013013No Hit
CTTGTTAAGCCTTTTAAGAAAAAAGTTATTAACTTAAGGAATTATAACAA103220.3548149579151373No Hit
GGAAAATCCCTTGGTTTGTGCAAGCTTACCTTGGGAATTGTCATGTTGAG100660.3460150519641321No Hit
CTACGGAAAAGCTGTAGCTAATACTTGCTTTAGCACTCTTGATTCATTTC93010.31971845800898No Hit
AAACGACTTAAGAGGTGTAGCATAAGTGGGAGTAGAGACTGAAATATGTT91670.31511225723775077No Hit
CGAAAGGGAAACAGGTTGATATTCCTGTGCCGATAGTATTTTGAGTGTAA91380.31411539289173845No Hit
CTACTTGACATTTTTTCTATAAGGATAACTACGGAAAAGCTGTAGCTAAT90660.3116404193430183No Hit
GGAGTCTAGCAAATGTGCAAGTGTGTATGATATGTTAAACATTTGGTACT90190.3100248116098259No Hit
CTGAAATATGTTTTTACGACAGTGAAATACCACTACTTTTAAAGTTGTTT83130.2857563209793195No Hit
CTTGACATTTTTTCTATAAGGATAACTACGGAAAAGCTGTAGCTAATACT82710.28431258640923274No Hit
ATCTACTTGACATTTTTTCTATAAGGATAACTACGGAAAAGCTGTAGCTA82570.2838313415525372No Hit
GGAAAATCCCTCGGTTTGTGCAAGCTTACCTTGGGAATTGTCATGTTGAG81720.2809094977797425No Hit
CTTTACTCTAGTCTGGCTTTGTGAAACGACTTAAGAGGTGTAGCATAAGT76170.26183157667502427No Hit
AATCAATTTACTTGGAGAAGAGGTTCGCTGAATCAATTCAGGCAAATTAT75060.25801599245408063No Hit
CAATTTACTTGGAGAAGAGGTTCGCTGAATCAATTCAGGCAAATTATTTT74440.25588476523157155No Hit
GGAAAAGCTGTAGCTAATACTTGCTTTAGCACTCTTGATTCATTTCTTGA73630.25310041998926136No Hit
CTTGGAATCAATTTACTTGGAGAAGAGGTTCGCTGAATCAATTCAGGCAA73540.25279104829567134No Hit
GTTAGCCGCTCCTAAGGGATAGCTGAAAAGTGTTTATTTGGGGAATGTTT69450.23873182355363576No Hit
CTTGAGTGTGTACTTGTTAAGCCTTTTAAGAAAAAAGTTATTAACTTAAG69190.2378380831054868No Hit
AGCAAACCTTGGAATCAATTTACTTGGAGAAGAGGTTCGCTGAATCAATT68920.23690996802471675No Hit
GGTTTCTTGTCAACTGTGATTGGACAAGAGTTAGCCGCTCCTAAGGGATA66080.2271475723603204No Hit
CATAATCCTATCACCTTTTTCGTGAGCTGCTGTTCGTGAGCTGTGAGCTG65840.2263225811774137No Hit
GTTAAAACGCTCGTAGTTGAATTTCAAAGAATCGATATTTTAAGCAACGC62060.21332897004663265No Hit
AAAAGGACCAGCTCAAAAGGAAAATCCCTCGGTTTGTGCAAGCTTACCTT61700.2120914832722725No Hit
CATTTCTTGAGTGTGTACTTGTTAAGCCTTTTAAGAAAAAAGTTATTAAC61550.21157586378295584No Hit
CTTGAATTCTTAATTTCCTGTTTTGTAGTAATAGTAATTCGTTTTTATGA60990.20965088435617343No Hit
CGGAAAAGCTGTAGCTAATACTTGCTTTAGCACTCTTGATTCATTTCTTG60770.20889464243850894No Hit
ATTTTTTTGGGGTTTCGGCCTTGAATTCTTAATTTCCTGTTTTGTAGTAA59680.20514780748280753No Hit
AGAAGACCCTGTTGAGCTTTACTCTAGTCTGGCTTTGTGAAACGACTTAA59600.2048728104218386No Hit
GAGAAATTATACGTGCTTCCTCTACGGATGCATTCATAATCCTATCACCT59260.20370407291272075No Hit
CTTGGAGAAGAGGTTCGCTGAATCAATTCAGGCAAATTATTTTTTTGGGG58700.20177909348593837No Hit
GTTACTTTGAGTAAATTAGAGTGTTCAAAGCAAACAGATATAGCATTGCG57230.19672602249063464No Hit
AACGACTTAAGAGGTGTAGCATAAGTGGGAGTAGAGACTGAAATATGTTT56360.19373542945259772No Hit
GGATAACTACGGAAAAGCTGTAGCTAATACTTGCTTTAGCACTCTTGATT56340.1936666801873555No Hit
CTTGATTCATTTCTTGAGTGTGTACTTGTTAAGCCTTTTAAGAAAAAAGT55180.1896792228033063No Hit
GTTGAGTAATTTTCCAATTAATGCCTTAGCATTCAAGCGAAGTGAGCAGG55000.18906047941612622No Hit
CTTGTCAACTGTGATTGGACAAGAGTTAGCCGCTCCTAAGGGATAGCTGA54970.1889573555182629No Hit
GTTGGTTCAGACAATTGGTACAGCAATTGGTTGACAAACCAGTGTTGCGA54710.18806361507011396No Hit
GAAAGATCTCTCAGGATCGCTGGAGTTGACTAATATAATTTTACCAGGTA54640.18782299264176613No Hit
CAAACCTTGGAATCAATTTACTTGGAGAAGAGGTTCGCTGAATCAATTCA51970.17864496573192873No Hit
AGAAATTATACGTGCTTCCTCTACGGATGCATTCATAATCCTATCACCTT51640.17751060285543196No Hit
AAGGAGTCTAGCAAATGTGCAAGTGTGTATGATATGTTAAACATTTGGTA51600.17737310432494754No Hit
ATTAAATAGTACCTGAAATCGTTAAGATGGAACGGATTAAGAGAGAAAAA50890.17493250540884844No Hit
GCAAGCTTACCTTGGGAATTGTCATGTTGAGTAATTTTCCAATTAATGCC50710.17431376202166837No Hit
CGACTTAAGAGGTGTAGCATAAGTGGGAGTAGAGACTGAAATATGTTTTT50000.17187316310556933No Hit
GTAAAAGAGAAATTATACGTGCTTCCTCTACGGATGCATTCATAATCCTA49300.16946693882209132No Hit
GTCTAGCAAATGTGCAAGTGTGTATGATATGTTAAACATTTGGTACTTCG49020.16850444910870016No Hit
GTTAAAACGCTCGTAGTTGAATTTCAAAGAATCGACATTTTAAGCAACGC48630.1671638384364767No Hit
GTTCGCTGAATCAATTCAGGCAAATTATTTTTTTGGGGTTTCGGCCTTGA46130.15857018028119824No Hit
GTAAGAAATATGCATTTCATCCCCGTCTCGAAAGGGAAACAGGTTGATAT45990.15808893542450267No Hit
ATTCGGTTGATTTGCTTACTTTGAAGAAAATATTGGGAAACGTAACAGTT44920.15441084973404345No Hit
CAAAAGGAAAATCCCTTGGTTTGTGCAAGCTTACCTTGGGAATTGTCATG43740.15035464308475202No Hit
ACTTGACATTTTTTCTATAAGGATAACTACGGAAAAGCTGTAGCTAATAC43030.14791404416865295No Hit
CTGAAAAGTGTTTATTTGGGGAATGTTTTGTAAGAAAATATGCATTTCAT42420.145817191578765No Hit
CTGAATTCGGTTGATTTGCTTACTTTGAAGAAAATATTGGGAAACGTAAC42150.14488907649799493No Hit
GACAAGAGTTAGCCGCTCCTAAGGGATAGCTGAAAAGTGTTTATTTGGGG41760.1435484658257715No Hit
CAAAAGGAAAATCCCTCGGTTTGTGCAAGCTTACCTTGGGAATTGTCATG41500.14265472537762253No Hit
CTTAATTTCCTGTTTTGTAGTAATAGTAATTCGTTTTTATGAATTATCCG41340.1421047312556847No Hit
CTCAGGATCGCTGGAGTTGACTAATATAATTTTACCAGGTAGAGATAATG40930.14069537131821905No Hit
CTGGAGTTGACTAATATAATTTTACCAGGTAGAGATAATGATTAGAGGAA40900.1405922474203557No Hit
CTGAAAAGTGTTTATTTGGGGAATGTTTTGTAAGAAATATGCATTTCATC40670.13980163087007008No Hit
CCTCGGTTTGTGCAAGCTTACCTTGGGAATTGTCATGTTGAGTAATTTTC40160.13804852460639327No Hit
GTGGAGAAGGGTTTCTTGTCAACTGTGATTGGACAAGAGTTAGCCGCTCC40030.1376016543823188No Hit
GTTGAATTTCAAAGAATCGATATTTTAAGCAACGCTTCTAGCTTAATCCA39020.1341298164875863No Hit
GTTTCTTTTTCGCGTAAGAATGTATTTGCTTGATTGTAAAGCTTCTTAGA38890.1336829462635118No Hit
CTTTTACTGCTGATGTAAAAGAGAAATTATACGTGCTTCCTCTACGGATG38570.13258295801963615No Hit
CTTAAGAGGTGTAGCATAAGTGGGAGTAGAGACTGAAATATGTTTTTACG38020.1306923532254749No Hit
GTTCAAAGCAAACAGATATAGCATTGCGCGTTTGAATACTACAGCATGGA37920.13034860689926378No Hit
ATTTACTATTATTTTCTTATACATATAGTATTGTGACAGTAATCCAACTT37900.13027985763402153No Hit
CAGCGAACTTGGATAATTATGATAAAACAATTCACTGAAGTTGTTGGGAA37340.12835487820723918No Hit
CCTTGGTTTGTGCAAGCTTACCTTGGGAATTGTCATGTTGAGTAATTTTC36940.12697989290239461No Hit
GGCAAATTATTTTTTTGGGGTTTCGGCCTTGAATTCTTAATTTCCTGTTT36860.1267048958414257No Hit
CGGGTATTCATATTAATTGAATTAATAAGTGGCACTTCTTATAGTGTTTC36790.1264642734130779No Hit
GGGTTTCTTGTCAACTGTGATTGGACAAGAGTTAGCCGCTCCTAAGGGAT36740.12629240024997232No Hit
AGGGTTTCTTGTCAACTGTGATTGGACAAGAGTTAGCCGCTCCTAAGGGA36720.1262236509847301No Hit
GTTTATTTGGGGAATGTTTTGTAAGAAAATATGCATTTCATCCCCGTCTC36620.12587990465851895No Hit
TGAAAGTATATGCATATTTTATATGTAGAAACTGCGAACGGCTCATTAAA35580.12230494286592312No Hit
TTTCTTGTCAACTGTGATTGGACAAGAGTTAGCCGCTCCTAAGGGATAGC35560.12223619360068089No Hit
GGAACATCAGCGAACTTGGATAATTATGATAAAACAATTCACTGAAGTTG35540.12216744433543866No Hit
GCTAAGTCTGTTGGATAATGGCTGAACGCCTCTTAAGCCAGAAACCATGC35310.12137682778515305No Hit
GTACTTGTTAAGCCTTTTAAGAAAAAAGTTATTAACTTAAGGAATTATAA35110.12068933513273077No Hit
CGAAAGATCTCTCAGGATCGCTGGAGTTGACTAATATAATTTTACCAGGT35070.1205518366022463No Hit
GTTAAGCCTTTTAAGAAAAAAGTTATTAACTTAAGGAATTATAACAAAGA34770.1195205976236129No Hit
CTCTAGTCTGGCTTTGTGAAACGACTTAAGAGGTGTAGCATAAGTGGGAG34730.11938309909312844No Hit
GTTTATTTGGGGAATGTTTTGTAAGAAATATGCATTTCATCCCCGTCTCG34700.1192799751952651No Hit
AGTAATTCGTTTTTATGAATTATCCGAAGCCGTAAGAACTATCCTTGAAA34420.11831748548187392No Hit
GTTGATATTCCTGTGCCGATAGTATTTTGAGTGTAATAGGGATGGTAACA34240.11769874209469386No Hit
CTTGGAAGTCCTTGTTACTTTGAGTAAATTAGAGTGTTCAAAGCAAACAG34240.11769874209469386No Hit
TCTTGATTCATTTCTTGAGTGTGTACTTGTTAAGCCTTTTAAGAAAAAAG34190.1175268689315883No Hit
GTTGATATTCCTGTGCCGATAGTATTTTGAGTGTAATAGAGATGGTAACA34190.1175268689315883No Hit
GTTTGACTGGGGCGGTACATCTGTTAAACAATAACGCAGATGTCCAAAGA34170.11745811966634608No Hit
GGGAAACAGGTTGATATTCCTGTGCCGATAGTATTTTGAGTGTAATAGGG33990.11683937627916602No Hit
AGAAAAGAAAATGGTTGAAAATAAAGGTATCGATGTATCCTTAGATTTCT33940.11666750311606046No Hit
CAAGGACCAGCTCAAAAGGAAAATCCCTTGGTTTGTGCAAGCTTACCTTG33680.11577376266791149No Hit
TGTGAAACGACTTAAGAGGTGTAGCATAAGTGGGAGTAGAGACTGAAATA33650.11567063877004816No Hit
GAAACGACTTAAGAGGTGTAGCATAAGTGGGAGTAGAGACTGAAATATGT33470.11505189538286809No Hit
GAAAGTATATGCATATTTTATATGTAGAAACTGCGAACGGCTCATTAAAA32730.11250817256890568No Hit
CTTACCTTGGGAATTGTCATGTTGAGTAATTTTCCAATTAATGCCTTAGC32000.10999882438756435No Hit
CAGTAATCCAACTTGGTACGAGAGGATTAGTTGGTTCAGACAATTGGTAC31860.10951757953086877No Hit
GGTTGATTTGCTTACTTTGAAGAAAATATTGGGAAACGTAACAGTTTCCC31780.10924258246989985No Hit
GTTTAAGGCAACAACAGGTCTGTGATGTCCTTAGATGAACTAGGCTGCAC31650.10879571224582536No Hit
CCCAATTCTAAAGAAGAGAGGTAGTGACAAGAAATAACAATACAAGGCCA31480.10821134349126645No Hit
AGAGGTTCGCTGAATCAATTCAGGCAAATTATTTTTTTGGGGTTTCGGCC31060.10676760892117966No Hit
GGGAAACAGGTTGATATTCCTGTGCCGATAGTATTTTGAGTGTAATAGAG30800.1058738684730307No Hit
GGGAAATCGTTTTCCTTGACCTATTCTCAAACTCCCAATAGGTAAAAAGG30710.10556449677944069No Hit
GGCTGAACGCCTCTTAAGCCAGAAACCATGCTGATTAAACAATACTATCT30620.10525512508585064No Hit
AATCTACTTGACATTTTTTCTATAAGGATAACTACGGAAAAGCTGTAGCT30420.10456763243342837No Hit
GACAATTGGTACAGCAATTGGTTGACAAACCAGTGTTGCGAAGCTAAGTC30310.10418951147459611No Hit
GGTTGATATTCCTGTGCCGATAGTATTTTGAGTGTAATAGGGATGGTAAC30240.10394888904624831No Hit
ATACAAATGAACTCCTTCACATAGGTCTTACACTCGGGGTGCGTTTTCTT29990.10308952323072047No Hit
GAAGGGTTTCTTGTCAACTGTGATTGGACAAGAGTTAGCCGCTCCTAAGG29830.10253952910878265No Hit
GGAGAAGGGTTTCTTGTCAACTGTGATTGGACAAGAGTTAGCCGCTCCTA29830.10253952910878265No Hit
GTTTTGTAAGAAATATGCATTTCATCCCCGTCTCGAAAGGGAAACAGGTT29640.10188641108898149No Hit
GAAAAAGGACCAGCTCAAAAGGAAAATCCCTCGGTTTGTGCAAGCTTACC29490.10137079159966479No Hit
GATATGTTAAACATTTGGTACTTCGTTACCTTCTATACGCACAATTAATG29450.10123329306918033No Hit
GTTGAATTTCAAAGAATCGACATTTTAAGCAACGCTTCTAGCTTAATCCA29420.10113016917131698No Hit
GGTTGATATTCCTGTGCCGATAGTATTTTGAGTGTAATAGAGATGGTAAC29330.10082079747772696No Hit
ATTTTACCAGGTAGAGATAATGATTAGAGGAATCAGGGAAATCGTTTTCC29270.10061454968200026No Hit
GGACATTAGAATAGAGAAGAAAAGAAAATGGTTGAAAATAAAGGTATCGA29140.1001676794579258No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)