FastQCFastQC Report
Thu 2 Nov 2023
HMLTGDMXY_n02_MHC-086.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHMLTGDMXY_n02_MHC-086.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences41951060
Sequences flagged as poor quality0
Sequence length101
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CCCAATTCTAAAGAAGAGAGGTAGTGACAAGAAATAACAATACAAGGCCA1556740.3710847830781868No Hit
GTTTGACTGGGGCGGTACATCTGTTAAACAATAACGCAGATGTCCAAAGA1025830.2445301739693824No Hit
AGAAGACCCTGTTGAGCTTTACTCTAGTCTGGCTTTGTGAAACGACTTAA963490.22967000118709752No Hit
CTTTACTCTAGTCTGGCTTTGTGAAACGACTTAAGAGGTGTAGCATAAGT913090.21765600201758906No Hit
GTTTCTTGTCAACTGTGATTGGACAAGAGTTAGCCGCTCCTAAGGGATAG830920.19806889265730115No Hit
GGAAAATCCCTCGGTTTGTGCAAGCTTACCTTGGGAATTGTCATGTTGAG797670.1901429904274171No Hit
GCCGAATTTAAGCATATAATTAAGCGGAAGAAAAGAAAATAACAATGATT796630.1898950825080463No Hit
CTTGTTAAGCCTTTTAAGAAAAAAGTTATTAACTTAAGGAATTATAACAA789590.18821693659230543No Hit
AATGGATCCGTCCATTTTTAGTGTGTATCAATCGAGTTTCTGACCTATCA768120.18309906829529457No Hit
GTGAAAGTATATGCATATTTTATATGTAGAAACTGCGAACGGCTCATTAA746160.17786439722858016No Hit
GTAACAACTCACCTACCGAATGAACTAGCCCTGAAAATGGATGGCGCTAA744290.1774186397197115No Hit
GTTTTCTTTGCACTTTACCTCTATAGCAAACCTTGGAATCAATTTACTTG743080.17713020839044352No Hit
TATTACTCGGTTCGCCGGGTATTCATATTAATTGAATTAATAAGTGGCAC741360.17672020683148412No Hit
AAACGACTTAAGAGGTGTAGCATAAGTGGGAGTAGAGACTGAAATATGTT726540.17318751898045007No Hit
CTCAAAAGGAAAATCCCTCGGTTTGTGCAAGCTTACCTTGGGAATTGTCA710530.16937116726013596No Hit
GTCCTTGTTACTTTGAGTAAATTAGAGTGTTCAAAGCAAACAGATATAGC710320.16932110893026303No Hit
CGACTTAAGAGGTGTAGCATAAGTGGGAGTAGAGACTGAAATATGTTTTT689590.16437963665280447No Hit
GGGAAATCGTTTTCCTTGACCTATTCTCAAACTCCCAATAGGTAAAAAGG678880.16182666182928393No Hit
CTGAAATATGTTTTTACGACAGTGAAATACCACTACTTTTAAAGTTGTTT671670.1601079925036459No Hit
AAAGAAGTGACACGTAATGGATCCGTCCATTTTTAGTGTGTATCAATCGA606590.1445946777030187No Hit
GGGGAATTAGAGTTCGATTCCGGAGAGGGAGCCTGAGAAATAGCTACCAC602850.14370316268528135No Hit
GGAGTCTAGCAAATGTGCAAGTGTGTATGATATGTTAAACATTTGGTACT587460.14003460222459216No Hit
GTTCGATTCCGGAGAGGGAGCCTGAGAAATAGCTACCACATCTAAGGAAG566040.13492865257755107No Hit
GTCTAGCAAATGTGCAAGTGTGTATGATATGTTAAACATTTGGTACTTCG564410.1345401045885372No Hit
AACGACTTAAGAGGTGTAGCATAAGTGGGAGTAGAGACTGAAATATGTTT554440.13216352578456897No Hit
GTTCAAAGCAAACAGATATAGCATTGCGCGTTTGAATACTACAGCATGGA550020.13110991712724304No Hit
GGACATTAGAATAGAGAAGAAAAGAAAATGGTTGAAAATAAAGGTATCGA548220.13068084572833202No Hit
CTGAGAAATAGCTACCACATCTAAGGAAGGCAGCAGGCGCGTAAATTACC543680.12959863231107865No Hit
GTCATGTTGAGTAATTTTCCAATTAATGCCTTAGCATTCAAGCGAAGTGA527540.1257512921008432No Hit
CAGCAGGCGCGTAAATTACCCAATTCTAAAGAAGAGAGGTAGTGACAAGA524490.12502425445268844No Hit
GTTCATTTCAGTCACGTTTAGGGGCGAAAGACTAATCGAAAAGCCTATTA518570.12361308629626998No Hit
CTTGGAGAAGAGGTTCGCTGAATCAATTCAGGCAAATTATTTTTTTGGGG517150.12327459663712906No Hit
TGTGAAACGACTTAAGAGGTGTAGCATAAGTGGGAGTAGAGACTGAAATA497770.11865492790885379No Hit
TAACAACTCACCTACCGAATGAACTAGCCCTGAAAATGGATGGCGCTAAA492790.11746783037186664No Hit
GTTAGTTTTACCCTACTGATGATGTGTTGTTGCCATGGTAATCCTGCTCA488890.11653817567422611No Hit
AGACCCTGTTGAGCTTTACTCTAGTCTGGCTTTGTGAAACGACTTAAGAG482070.11491247181835214No Hit
CCCTGTTGAGCTTTACTCTAGTCTGGCTTTGTGAAACGACTTAAGAGGTG481110.11468363373893294No Hit
CTTGGAATCAATTTACTTGGAGAAGAGGTTCGCTGAATCAATTCAGGCAA479220.11423310877007636No Hit
CAAAGAAGTGACACGTAATGGATCCGTCCATTTTTAGTGTGTATCAATCG474610.11313420924286538No Hit
GGGAATTAGAGTTCGATTCCGGAGAGGGAGCCTGAGAAATAGCTACCACA470350.11211874026544263No Hit
GTTACTTTGAGTAAATTAGAGTGTTCAAAGCAAACAGATATAGCATTGCG469420.11189705337600528No Hit
GAAGACCCTGTTGAGCTTTACTCTAGTCTGGCTTTGTGAAACGACTTAAG466000.11108181771807435No Hit
ATTTACTTGGAGAAGAGGTTCGCTGAATCAATTCAGGCAAATTATTTTTT460800.1098422781212203No Hit
AGCAAACCTTGGAATCAATTTACTTGGAGAAGAGGTTCGCTGAATCAATT451660.10766354890674992No Hit
GCATAAGTGGGAGTAGAGACTGAAATATGTTTTTACGACAGTGAAATACC426290.10161602591209852No Hit
GACCCTGTTGAGCTTTACTCTAGTCTGGCTTTGTGAAACGACTTAAGAGG424640.10122271046309676No Hit
GTTCGCTGAATCAATTCAGGCAAATTATTTTTTTGGGGTTTCGGCCTTGA422640.10074596446430675No Hit
GTTTAAGGCAACAACAGGTCTGTGATGTCCTTAGATGAACTAGGCTGCAC421110.10038125377523238No Hit

[WARN]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)