FastQCFastQC Report
Thu 2 Nov 2023
HMLTGDMXY_n02_MHC-085.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHMLTGDMXY_n02_MHC-085.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3496743
Sequences flagged as poor quality0
Sequence length101
%GC42

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GTTTCTTGTCAACTGTGATTGGACAAGAGTTAGCCGCTCCTAAGGGATAG528201.5105485304467614No Hit
CTCAAAAGGAAAATCCCTCGGTTTGTGCAAGCTTACCTTGGGAATTGTCA331170.9470813268232752No Hit
GGTTTCTTGTCAACTGTGATTGGACAAGAGTTAGCCGCTCCTAAGGGATA306670.8770161261493911No Hit
GTTTTCTTTGCACTTTACCTCTATAGCAAACCTTGGAATCAATTTACTTG268170.7669136679475729No Hit
CCTCGGTTTGTGCAAGCTTACCTTGGGAATTGTCATGTTGAGTAATTTTC240160.6868105548506138No Hit
TTTCTTGTCAACTGTGATTGGACAAGAGTTAGCCGCTCCTAAGGGATAGC174910.5002083367293507No Hit
GCAAGCTTACCTTGGGAATTGTCATGTTGAGTAATTTTCCAATTAATGCC169870.4857949240192946No Hit
GTTAGCCGCTCCTAAGGGATAGCTGAAAAGTGTTTATTTGGGGAATGTTT154590.44209711723166384No Hit
CTTGGAATCAATTTACTTGGAGAAGAGGTTCGCTGAATCAATTCAGGCAA147140.4207915766185848No Hit
GTCCTTGTTACTTTGAGTAAATTAGAGTGTTCAAAGCAAACAGATATAGC142180.4066069482372596No Hit
GGGTTTCTTGTCAACTGTGATTGGACAAGAGTTAGCCGCTCCTAAGGGAT116400.33288119830367857No Hit
GTGCAAGTGTGTATGATATGTTAAACATCTGGTACTTCGTTACCTTCTAT115970.3316514825367492No Hit
GTGCAAGTGTGTATGATATGTTAAACATTTGGTACTTCGCTACCTTCTAT113700.32515972720900566No Hit
GTTGGTTCAGACAATTGGTACAGCAATTGGTTGACAAACCAGTGTTGCGA109180.3122334126357013No Hit
CTCGGTTTGTGCAAGCTTACCTTGGGAATTGTCATGTTGAGTAATTTTCC108360.309888373266208No Hit
TGTCATGTTGAGTAATTTTCCAATTAATGCCTTAGCATTCAAGCGAAGTG102890.2942452447892224No Hit
GGAAAATCCCTCGGTTTGTGCAAGCTTACCTTGGGAATTGTCATGTTGAG97850.27983183207916623No Hit
GTTCGCTGAATCAATTCAGGCAAATTATTTTTTTGGGGTTTCGGCCTTGA93670.26787785090296884No Hit
TTTTCTTTGCACTTTACCTCTATAGCAAACCTTGGAATCAATTTACTTGG90390.2584976934249958No Hit
CTTGTCAACTGTGATTGGACAAGAGTTAGCCGCTCCTAAGGGATAGCTGA87060.2489745457415658No Hit
CCTTGTTACTTTGAGTAAATTAGAGTGTTCAAAGCAAACAGATATAGCAT85410.24425586896148788No Hit
GTTGAATTTCAAAGAATCGACATTTTAAGCAACGCTTCTAGCTTAATCCA82960.23724934889409946No Hit
TGTGCAAGTGTGTATGATATGTTAAACATCTGGTACTTCGTTACCTTCTA82790.23676318219554596No Hit
TTCTTGTCAACTGTGATTGGACAAGAGTTAGCCGCTCCTAAGGGATAGCT82640.23633421157917525No Hit
CTTGGAGAAGAGGTTCGCTGAATCAATTCAGGCAAATTATTTTTTTGGGG81580.2333028192234888No Hit
GGTTGATATTCCTGTGCCGATAGTATTTTGAGTGTAATAGAGATGGTAAC81490.23304543685366638No Hit
CTTGACATTTTTTCTATAAGGATAACTACGGAAAAGCTGTAGCTAATACT81060.23181572108673698No Hit
TGTGCAAGTGTGTATGATATGTTAAACATTTGGTACTTCGCTACCTTCTA80560.23038581903216793No Hit
CAGCGAACTTGGATAATTATGATAAAACAATTCACTGAAGTTGTTGGGAA78680.22500938730698825No Hit
GGCTGAACGCCTCTTAAGCCAGAAACCATGCTGATTAAACAATACTATCT78140.22346509308805365No Hit
TTTCGAAAGATCTCTCAGGATCGCTGGAGTTGACTAATATAATTTTACCA76140.2177454848697774No Hit
TTTCTTTGCACTTTACCTCTATAGCAAACCTTGGAATCAATTTACTTGGA75760.21665875930830492No Hit
GCTTAATCCACATAACTGATACTTCGTATCGACTTTGTGCGCATTTTGCT74620.21339858262388745No Hit
GCTCGTAGTTGAATTTCAAAGAATCGACATTTTAAGCAACGCTTCTAGCT74410.21279802376096843No Hit
TGCTGATTAAACAATACTATCTCGTTCTTTTTTGTTATCCTTTTACTGCT74050.21176849428167868No Hit
TCTACTTGACATTTTTTCTATAAGGATAACTACGGAAAAGCTGTAGCTAA72820.2082509352274388No Hit
GTCATGTTGAGTAATTTTCCAATTAATGCCTTAGCATTCAAGCGAAGTGA70740.2023025426804315No Hit
TTTTGCTATTATGTGTTCTTTTAATTAAAATGATTCTTTTTAAGGACTTT69520.19881358166728294No Hit
CGCTCGTAGTTGAATTTCAAAGAATCGACATTTTAAGCAACGCTTCTAGC67080.19183565964098592No Hit
CTACTTGACATTTTTTCTATAAGGATAACTACGGAAAAGCTGTAGCTAAT66040.18886146336748225No Hit
CGCCTCTTAAGCCAGAAACCATGCTGATTAAACAATACTATCTCGTTCTT63030.18025345299897647No Hit
GTTCTTTTAATTAAAATGATTCTTTTTAAGGACTTTCTTTGCTTCGGCTT62620.17908093331422983No Hit
CTTACCTTGGGAATTGTCATGTTGAGTAATTTTCCAATTAATGCCTTAGC61720.17650710961600552No Hit
TGTTGAGTAATTTTCCAATTAATGCCTTAGCATTCAAGCGAAGTGAGCAG61200.1750200114792537No Hit
GCTGAACGCCTCTTAAGCCAGAAACCATGCTGATTAAACAATACTATCTC60990.1744194526163347No Hit
TTTTGAGTGTAATAGAGATGGTAACATATATACAAATGAACTCCTTCACA60260.17233179561666384No Hit
CTTGGAAGTCCTTGTTACTTTGAGTAAATTAGAGTGTTCAAAGCAAACAG59950.17144525634283103No Hit
GCTTACCTTGGGAATTGTCATGTTGAGTAATTTTCCAATTAATGCCTTAG59620.17050152098681542No Hit
GTTGAGTAATTTTCCAATTAATGCCTTAGCATTCAAGCGAAGTGAGCAGG58690.167841903165317No Hit
TGGGAATTGTCATGTTGAGTAATTTTCCAATTAATGCCTTAGCATTCAAG58160.16632620698747377No Hit
GTTAAAACGCTCGTAGTTGAATTTCAAAGAATCGACATTTTAAGCAACGC57460.16432434411107708No Hit
GTGGAGAAGGGTTTCTTGTCAACTGTGATTGGACAAGAGTTAGCCGCTCC56100.16043501052264922No Hit
CCTTCACATAGGTCTTACACTCGGGGTGCGTTTTCTTTGCACTTTACCTC56040.16026342227610094No Hit
GCTAAGTCTGTTGGATAATGGCTGAACGCCTCTTAAGCCAGAAACCATGC55050.1574322162080542No Hit
ATTTACTTGGAGAAGAGGTTCGCTGAATCAATTCAGGCAAATTATTTTTT54060.15460101014000743No Hit
GTTCGCTGAATCAATTCAGGCAAATTATTTTTTTGGGGTTTCGGCTCTGA53780.15380026498944877No Hit
TTTCCAATTAATGCCTTAGCATTCAAGCGAAGTGAGCAGGAAAGCTCTCC53710.15360007870180908No Hit
TTTCAAAGAATCGACATTTTAAGCAACGCTTCTAGCTTAATCCACATAAC53020.15162681386650376No Hit
CTTGGGAATTGTCATGTTGAGTAATTTTCCAATTAATGCCTTAGCATTCA52400.14985373531883814No Hit
CGCTGAATCAATTCAGGCAAATTATTTTTTTGGGGTTTCGGCCTTGAATT49850.1425612348405359No Hit
TCTTGATTCATTTCTTGAGTGTGTACTTGTTAAGCCTTTTAAGAAAAAAG49660.14201787205979965No Hit
GCTCAAAAGGAAAATCCCTCGGTTTGTGCAAGCTTACCTTGGGAATTGTC49620.14190347989543411No Hit
AGCGAACTTGGATAATTATGATAAAACAATTCACTGAAGTTGTTGGGAAT49480.14150310732015478No Hit
GCGGCAAATACGATATATTCTTACGTGGGACTGAATTCGGTTGATTTGCT48310.13815713651246317No Hit
ATCTACTTGACATTTTTTCTATAAGGATAACTACGGAAAAGCTGTAGCTA48180.1377853619782752No Hit
CTTTTACTGCTGATGTAAAAGAGAAATTATACGTGCTTCCTCTACGGATG47040.13452518529385774No Hit
TGGAGAAGGGTTTCTTGTCAACTGTGATTGGACAAGAGTTAGCCGCTCCT47020.13446798921167497No Hit
TGCTGATTAAACAATACTATCTCGTTCTTTTTTGTTATGCTTTTACTGCT46870.13403901859530426No Hit
CCAGCTCAAAAGGAAAATCCCTCGGTTTGTGCAAGCTTACCTTGGGAATT46850.1339818225131215No Hit
TTGGGAATTGTCATGTTGAGTAATTTTCCAATTAATGCCTTAGCATTCAA46500.13298089107492314No Hit
TTTTAAGCAACGCTTCTAGCTTAATCCACATAACTGATACTTCGTATCGA46020.13160818510253686No Hit
CGGAAAAGCTGTAGCTAATACTTGCTTTAGCACTCTTGATTCATTTCTTG45610.13043566541779023No Hit
GCTGAAAAGTGTTTATTTGGGGAATGTTTTGTAAGAAATATGCATTTCAT45530.13020688108905917No Hit
CCCTCGGTTTGTGCAAGCTTACCTTGGGAATTGTCATGTTGAGTAATTTT44790.12809062604829693No Hit
CTTGTTACTTTGAGTAAATTAGAGTGTTCAAAGCAAACAGATATAGCATT44750.12797623388393142No Hit
TCAAAAGGAAAATCCCTCGGTTTGTGCAAGCTTACCTTGGGAATTGTCAT44270.1266035279115451No Hit
CTTGTTAAGCCTTTTAAGAAAAAAGTTATTAACTTAAGGAATTATAACAA43710.12500203761042777No Hit
CTCGGGGTGCGTTTTCTTTGCACTTTACCTCTATAGCAAACCTTGGAATC42190.12065513536453781No Hit
CTCGTAGTTGAATTTCAAAGAATCGACATTTTAAGCAACGCTTCTAGCTT42100.12039775299471536No Hit
ACTTGACATTTTTTCTATAAGGATAACTACGGAAAAGCTGTAGCTAATAC41580.11891065485796354No Hit
TGCTGATGTAAAAGAGAAATTATACGTGCTTCCTCTACGGATGCATTCAT41340.1182243018717704No Hit
TTTTGAGTGTAATAGAGATGGTAACATACATACAAATGAACTCCTTCACA40840.11679439981720133No Hit
CTGAAAAGTGTTTATTTGGGGAATGTTTTGTAAGAAATATGCATTTCATC40580.11605085074882542No Hit
GTGCAAGTGTGTATGATATGTTAAACATTTGGTACTTCGTTGCCTTCTAT40270.11516431147499259No Hit
CAGTAATCCAACTTGGTACGAGAGGATTAGTTGGTTCAGACAATTGGTAC40070.11459235065316496No Hit
GTTCAAAGCAAACAGATATAGCATTGCGCGTTTGAATACTACAGCATGGA39850.11396319374915456No Hit
TGTCAACTGTGATTGGACAAGAGTTAGCCGCTCCTAAGGGATAGCTGAAA39160.11198992891384926No Hit
GCGAACTTGGATAATTATGATAAAACAATTCACTGAAGTTGTTGGGAATT38990.11150376221529577No Hit
CTGATACTTCGTATCGACTTTGTGCGCATTTTGCTATTATGTGTTCTTTT38820.1110175955167423No Hit
CTTTGCACTTTACCTCTATAGCAAACCTTGGAATCAATTTACTTGGAGAA38790.11093180139346816No Hit
TTTTTCTATAAGGATAACTACGGAAAAGCTGTAGCTAATACTTGCTTTAG37580.107471438421411No Hit
TTTACTATTATTTTCTTATACATATAGTATTGTGACAGTAATCCAACTTG37130.10618452657229886No Hit
GTTGGATAATGGCTGAACGCCTCTTAAGCCAGAAACCATGCTGATTAAAC37120.10615592853120746No Hit
TTTTACTGCTGATGTAAAAGAGAAATTATACGTGCTTCCTCTACGGATGC36990.10578415399701951No Hit
CTTGCTTTAGCACTCTTGATTCATTTCTTGAGTGTGTACTTGTTAAGCCT35970.10286715380569862No Hit
GGAACATCAGCGAACTTGGATAATTATGATAAAACAATTCACTGAAGTTG35760.1022665949427796No Hit
TGTGACAGTAATCCAACTTGGTACGAGAGGATTAGTTGGTTCAGACAATT35410.10126566350458126No Hit
CTTTCTTTGCTTCGGCTTGGAAGTCCTTGTTACTTTGAGTAAATTAGAGT35360.10112267329912435No Hit
GGTTCGCTGAATCAATTCAGGCAAATTATTTTTTTGGGGTTTCGGCCTTG35280.10089388897039332No Hit
ATTCAAGCGAAGTGAGCAGGAAAGCTCTCCCTAAGAGGGTGAAAGGCCCG35270.10086529092930192No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)