FastQCFastQC Report
Thu 2 Nov 2023
HMLTGDMXY_n02_MHC-076.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHMLTGDMXY_n02_MHC-076.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1623916
Sequences flagged as poor quality0
Sequence length101
%GC41

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GTTTCTTGTCAACTGTGATTGGACAAGAGTTAGCCGCTCCTAAGGGATAG232311.4305542897539036No Hit
CTCAAAAGGAAAATCCCTCGGTTTGTGCAAGCTTACCTTGGGAATTGTCA156560.9640892755536615No Hit
GTTTTCTTTGCACTTTACCTCTATAGCAAACCTTGGAATCAATTTACTTG130230.8019503471854456No Hit
GGTTTCTTGTCAACTGTGATTGGACAAGAGTTAGCCGCTCCTAAGGGATA122700.7555809536946493No Hit
CCTCGGTTTGTGCAAGCTTACCTTGGGAATTGTCATGTTGAGTAATTTTC91770.5651154370053624No Hit
GTCCTTGTTACTTTGAGTAAATTAGAGTGTTCAAAGCAAACAGATATAGC86230.5310003719404205No Hit
GTGCAAGTGTGTATGAAATGTTAAACATTTGGTACTTCGCTACCTTCTAT84440.5199776343111343No Hit
CCAGCTCAAAAGGAAAATCCCTCGGTTTGTGCAAGCTTACCTTGGGAATT82550.508339101283564No Hit
TTTCTTGTCAACTGTGATTGGACAAGAGTTAGCCGCTCCTAAGGGATAGC81400.5012574542032963No Hit
GCAAGCTTACCTTGGGAATTGTCATGTTGAGTAATTTTCCAATTAATGCC78890.4858009897063641No Hit
GTTCGCTGAATCAATTCAGGCAAATTATTTTTTTGGGGTTTCGGCCTTGA75310.4637555144477916No Hit
CTTGGAATCAATTTACTTGGAGAAGAGGTTCGCTGAATCAATTCAGGCAA71760.4418947778087044No Hit
GTTGAATTTCAAAGAATCGATATTTTAAGCAACGCTTCTAGCTTAATCCA68420.4213272115060138No Hit
TGTGCAAGTGTGTATGAAATGTTAAACATTTGGTACTTCGCTACCTTCTA65750.4048854743718271No Hit
GTTAGCCGCTCCTAAGGGATAGCTGAAAAGTGTTTATTTGGGGAATGTTT64450.3968801341941332No Hit
GCTCGTAGTTGAATTTCAAAGAATCGATATTTTAAGCAACGCTTCTAGCT56130.3456459570568921No Hit
TGCTGATTAAACAATACTATCTCGTTCTTTTTTGTTATCCTTTTACTGCT55210.339980639392678No Hit
CCTTGTTACTTTGAGTAAATTAGAGTGTTCAAAGCAAACAGATATAGCAT54240.3340074240293217No Hit
GTTAAAACGCTCGTAGTTGAATTTCAAAGAATCGATATTTTAAGCAACGC53920.33203687875481247No Hit
CTTGACATTTTTTCTATAAGGATAACTACGGAAAAGCTGTAGCTAATACT51410.3165804142578803No Hit
CGCTCGTAGTTGAATTTCAAAGAATCGATATTTTAAGCAACGCTTCTAGC51360.3162725165587383No Hit
CTTGGAAGTCCTTGTTACTTTGAGTAAATTAGAGTGTTCAAAGCAAACAG50260.30949876717761265No Hit
GCTTAATCCACATAACTGATACTTCGTATCGACTTTGTGCGCATTTTGCT48630.29946130218558104No Hit
TTTCAAAGAATCGATATTTTAAGCAACGCTTCTAGCTTAATCCACATAAC48300.29742917737124336No Hit
TCTACTTGACATTTTTTCTATAAGGATAACTACGGAAAAGCTGTAGCTAA48270.2972444387517581No Hit
GGGTTTCTTGTCAACTGTGATTGGACAAGAGTTAGCCGCTCCTAAGGGAT46580.286837496520756No Hit
TTTTCTTTGCACTTTACCTCTATAGCAAACCTTGGAATCAATTTACTTGG45570.2806179629980861No Hit
GTTGGTTCAGACAATTGGTACAGCAATTGGTTGACAAACCAGTGTTGCGA45090.2776621450863222No Hit
GGAAAATCCCTCGGTTTGTGCAAGCTTACCTTGGGAATTGTCATGTTGAG44370.2732284182186763No Hit
CTTGGAGAAGAGGTTCGCTGAATCAATTCAGGCAAATTATTTTTTTGGGG43360.2670088846960064No Hit
CTCGGTTTGTGCAAGCTTACCTTGGGAATTGTCATGTTGAGTAATTTTCC41360.2546929767303235No Hit
TTTTGCTATTATGTGTTCTTTTAATTAAAATGATTCTTTTTAAGGACTTT41300.254323499491353No Hit
CTACTTGACATTTTTTCTATAAGGATAACTACGGAAAAGCTGTAGCTAAT41230.25389244271255407No Hit
TTTCTTTGCACTTTACCTCTATAGCAAACCTTGGAATCAATTTACTTGGA40830.2514292611194175No Hit
CTTGTCAACTGTGATTGGACAAGAGTTAGCCGCTCCTAAGGGATAGCTGA40560.2497666135440503No Hit
CGCTGAATCAATTCAGGCAAATTATTTTTTTGGGGTTTCGGCCTTGAATT39950.246010261614517No Hit
TTCTTGTCAACTGTGATTGGACAAGAGTTAGCCGCTCCTAAGGGATAGCT38800.23892861453424932No Hit
TGTCATGTTGAGTAATTTTCCAATTAATGCCTTAGCATTCAAGCGAAGTG38170.23504910352505917No Hit
GGCTGAACGCCTCTTAAGCCAGAAACCATGCTGATTAAACAATACTATCT38160.23498752398523076No Hit
ACCAGCTCAAAAGGAAAATCCCTCGGTTTGTGCAAGCTTACCTTGGGAAT37900.23338645594969198No Hit
GGTTGATATTCCTGTGCCGATAGTATTTTGAGTGTAATAGAGATGGTAAC37730.23233960377260893No Hit
CAGCTCAAAAGGAAAATCCCTCGGTTTGTGCAAGCTTACCTTGGGAATTG34570.21288046918682985No Hit
TTTCGAAAGATCTCTCAGGATCGCTGGAGTTGACTAATATAATTTTACCA34360.21158729885043312No Hit
GTTCTTTTAATTAAAATGATTCTTTTTAAGGACTTTCTTTGCTTCGGCTT34120.2101093898945512No Hit
GTGTGTATGAAATGTTAAACATTTGGTACTTCGCTACCTTCTATACGCAC33410.20573724256673373No Hit
GGACCAGCTCAAAAGGAAAATCCCTCGGTTTGTGCAAGCTTACCTTGGGA32470.19994876582286278No Hit
GACCAGCTCAAAAGGAAAATCCCTCGGTTTGTGCAAGCTTACCTTGGGAA31340.1929902778222519No Hit
CCTTCACATAGGTCTTACACTCGGGGTGCGTTTTCTTTGCACTTTACCTC30870.1900960394503164No Hit
CTCGTAGTTGAATTTCAAAGAATCGATATTTTAAGCAACGCTTCTAGCTT30380.18707864199872407No Hit
ATCTACTTGACATTTTTTCTATAAGGATAACTACGGAAAAGCTGTAGCTA29950.18443072178610223No Hit
CGGAAAAGCTGTAGCTAATACTTGCTTTAGCACTCTTGATTCATTTCTTG29900.18412282408696018No Hit
GCTAAGTCTGTTGGATAATGGCTGAACGCCTCTTAAGCCAGAAACCATGC29850.1838149263878181No Hit
TTTTGAGTGTAATAGAGATGGTAACATATATACAAATGAACTCCTTCACA29790.18344544914884758No Hit
CGCCTCTTAAGCCAGAAACCATGCTGATTAAACAATACTATCTCGTTCTT29420.18116700617519627No Hit
GCTGAACGCCTCTTAAGCCAGAAACCATGCTGATTAAACAATACTATCTC29370.18085910847605416No Hit
CTTTCTTTGCTTCGGCTTGGAAGTCCTTGTTACTTTGAGTAAATTAGAGT29000.17858066550240284No Hit
GTCATGTTGAGTAATTTTCCAATTAATGCCTTAGCATTCAAGCGAAGTGA28980.178457506422746No Hit
GGTTCGCTGAATCAATTCAGGCAAATTATTTTTTTGGGGTTTCGGCCTTG28560.17587116574995257No Hit
GCTCAAAAGGAAAATCCCTCGGTTTGTGCAAGCTTACCTTGGGAATTGTC27930.17199165474076245No Hit
ATTTACTTGGAGAAGAGGTTCGCTGAATCAATTCAGGCAAATTATTTTTT27460.16909741636882697No Hit
GTTCAAAGCAAACAGATATAGCATTGCGCGTTTGAATACTACAGCATGGA27180.16737318925363134No Hit
TTTTAAGCAACGCTTCTAGCTTAATCCACATAACTGATACTTCGTATCGA27060.16663423477569037No Hit
ACTTGACATTTTTTCTATAAGGATAACTACGGAAAAGCTGTAGCTAATAC26250.16164629204958877No Hit
TCTTGATTCATTTCTTGAGTGTGTACTTGTTAAGCCTTTTAAGAAAAAAG26170.16115365573096144No Hit
CTTGTTACTTTGAGTAAATTAGAGTGTTCAAAGCAAACAGATATAGCATT25880.15936784907593743No Hit
TTTTGAGTGTAATAGAGATGGTAACATACATACAAATGAACTCCTTCACA25340.15604255392520303No Hit
CTTGTTAAGCCTTTTAAGAAAAAAGTTATTAACTTAAGGAATTATAACAA25050.154256747270179No Hit
CGCGTGCTACACTGATATGTATAACGAGTTATTAAAATTACGATTCAGCT25030.15413358819052217No Hit
GCTGATTAAACAATACTATCTCGTTCTTTTTTGTTATCCTTTTACTGCTG24210.14908406592459214No Hit
TCGTAGTTGAATTTCAAAGAATCGATATTTTAAGCAACGCTTCTAGCTTA23660.14569719123402936No Hit
GTTGAGTAATTTTCCAATTAATGCCTTAGCATTCAAGCGAAGTGAGCAGG23640.1455740321543725No Hit
TGTTGAGTAATTTTCCAATTAATGCCTTAGCATTCAAGCGAAGTGAGCAG23360.1438498050391769No Hit
CTTACCTTGGGAATTGTCATGTTGAGTAATTTTCCAATTAATGCCTTAGC23060.14200241884432446No Hit
GTTACTTTGAGTAAATTAGAGTGTTCAAAGCAAACAGATATAGCATTGCG23040.14187925976466764No Hit
GCTGAATCAATTCAGGCAAATTATTTTTTTGGGGTTTCGGCCTTGAATTC22720.13990871449015835No Hit
GTGGAGAAGGGTTTCTTGTCAACTGTGATTGGACAAGAGTTAGCCGCTCC22440.13818448737496275No Hit
TGTGTATGAAATGTTAAACATTTGGTACTTCGCTACCTTCTATACGCACA22290.1372607942775365No Hit
CTTGAATTCTTAATTTCCTGTTTTGTAGTAATAGTAATTCGTTTTTATGA22160.13646026025976712No Hit
TGGGAATTGTCATGTTGAGTAATTTTCCAATTAATGCCTTAGCATTCAAG21590.1329502264895475No Hit
TGTGAAACGACTTAAGAGGTGTAGCATAAGTGGGAGTAGAAACTGAAATA21500.13239601063109177No Hit
TCAAAAGGAAAATCCCTCGGTTTGTGCAAGCTTACCTTGGGAATTGTCAT21450.1320881129319497No Hit
GCTTACCTTGGGAATTGTCATGTTGAGTAATTTTCCAATTAATGCCTTAG21440.13202653339212125No Hit
CTCGGGGTGCGTTTTCTTTGCACTTTACCTCTATAGCAAACCTTGGAATC21060.12968651087864153No Hit
CTTTTACTGCTGATGTAAAAGAGAAATTATACGTGCTTCCTCTACGGATG20790.1280238633032743No Hit
CTGATACTTCGTATCGACTTTGTGCGCATTTTGCTATTATGTGTTCTTTT20560.12660753388722076No Hit
CTAGGCTGCACGCGTGCTACACTGATATGTATAACGAGTTATTAAAATTA20180.12426751137374101No Hit
TTTGCTATTATGTGTTCTTTTAATTAAAATGATTCTTTTTAAGGACTTTC19740.12155801162129075No Hit
TGGAGAAGGGTTTCTTGTCAACTGTGATTGGACAAGAGTTAGCCGCTCCT19720.12143485254163391No Hit
TGTCAACTGTGATTGGACAAGAGTTAGCCGCTCCTAAGGGATAGCTGAAA19720.12143485254163391No Hit
ATTTTTTTGGGGTTTCGGCCTTGAATTCTTAATTTCCTGTTTTGTAGTAA19510.12014168220523722No Hit
CAGCGAACTTGGATAATTATGATAAAACAATTCACTGAAGTTGTTGGGAA19510.12014168220523722No Hit
CTTTGCACTTTACCTCTATAGCAAACCTTGGAATCAATTTACTTGGAGAA19280.11872535278918367No Hit
TTTTTCTATAAGGATAACTACGGAAAAGCTGTAGCTAATACTTGCTTTAG18850.11607743257656185No Hit
GGCAAATTATTTTTTTGGGGTTTCGGCCTTGAATTCTTAATTTCCTGTTT18630.11472268270033671No Hit
CTTGCTTTAGCACTCTTGATTCATTTCTTGAGTGTGTACTTGTTAAGCCT18420.11342951236394001No Hit
CTTGGGAATTGTCATGTTGAGTAATTTTCCAATTAATGCCTTAGCATTCA18320.11281371696565586No Hit
AGCAAACCTTGGAATCAATTTACTTGGAGAAGAGGTTCGCTGAATCAATT18260.11244423972668538No Hit
GCTGTAGCTAATACTTGCTTTAGCACTCTTGATTCATTTCTTGAGTGTGT18130.11164370570891599No Hit
TTTCCAATTAATGCCTTAGCATTCAAGCGAAGTGAGCAGGAAAGCTCTCC17720.10911894457595098No Hit
TTGACATTTTTTCTATAAGGATAACTACGGAAAAGCTGTAGCTAATACTT17660.10874946733698049No Hit
TTTTACTGCTGATGTAAAAGAGAAATTATACGTGCTTCCTCTACGGATGC17530.1079489333192111No Hit
GTTGGATAATGGCTGAACGCCTCTTAAGCCAGAAACCATGCTGATTAAAC17460.10751787654041219No Hit
GCGGCAAATACGATATATTCTTACGTGGGACTGAATTCGGTTGATTTGCT17240.10616312666418706No Hit
CCCGTCTCGAAAGGGAAACAGGTTGATATTCCTGTGCCGATAGTATTTTG17200.1059168085048734No Hit
CCCTCGGTTTGTGCAAGCTTACCTTGGGAATTGTCATGTTGAGTAATTTT17120.10542417218624608No Hit
TGCTGATGTAAAAGAGAAATTATACGTGCTTCCTCTACGGATGCATTCAT17040.10493153586761876No Hit
TGTTCTTTTAATTAAAATGATTCTTTTTAAGGACTTTCTTTGCTTCGGCT17040.10493153586761876No Hit
CTTTGCTTCGGCTTGGAAGTCCTTGTTACTTTGAGTAAATTAGAGTGTTC16840.10369994507105047No Hit
TTTGCACTTTACCTCTATAGCAAACCTTGGAATCAATTTACTTGGAGAAG16800.10345362691173682No Hit
TTGGGAATTGTCATGTTGAGTAATTTTCCAATTAATGCCTTAGCATTCAA16450.1012983430177423No Hit
CGACTTAAGAGGTGTAGCATAAGTGGGAGTAGAAACTGAAATATGTTTTT16290.10031307038048766No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)