FastQCFastQC Report
Thu 2 Nov 2023
HMLTGDMXY_n02_MHC-067.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHMLTGDMXY_n02_MHC-067.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1827389
Sequences flagged as poor quality0
Sequence length101
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTTTCTTGTCAACTGTGATTGGACAAGAGTTAGCCGCTCCTAAGGGATAG164120.8981120057086915No Hit
GGTTTCTTGTCAACTGTGATTGGACAAGAGTTAGCCGCTCCTAAGGGATA103050.5639193406548906No Hit
GTTTTCTTTGCACTTTACCTCTATAGCAAACCTTGGAATCAATTTACTTG95680.523588573642503No Hit
CTCAAAAGGAAAATCCCTCGGTTTGTGCAAGCTTACCTTGGGAATTGTCA80120.43843976296234677No Hit
TTTCTTGTCAACTGTGATTGGACAAGAGTTAGCCGCTCCTAAGGGATAGC57330.31372630567438026No Hit
GCAAGCTTACCTTGGGAATTGTCATGTTGAGTAATTTTCCAATTAATGCC53550.2930410547507947No Hit
CCTCGGTTTGTGCAAGCTTACCTTGGGAATTGTCATGTTGAGTAATTTTC50270.27509194812927074No Hit
GTTGGTTCAGACAATTGGTACAGCAATTGGTTGACAAACCAGTGTTGCGA48420.2649682142116429No Hit
GTTAGCCGCTCCTAAGGGATAGCTGAAAAGTGTTTATTTGGGGAATGTTT47940.2623415156816638No Hit
GGGTTTCTTGTCAACTGTGATTGGACAAGAGTTAGCCGCTCCTAAGGGAT43470.23788038562123337No Hit
GTTCGCTGAATCAATTCAGGCAAATTATTTTTTTGGGGTTTCGGCCTTGA40340.22075212229032787No Hit
TGCTGATTAAACAATACTATCTCGTTCTTTTTTGTTATCCTTTTACTGCT39230.21467788193975118No Hit
GTCCTTGTTACTTTGAGTAAATTAGAGTGTTCAAAGCAAACAGATATAGC39210.21456843616766874No Hit
CTTGGAATCAATTTACTTGGAGAAGAGGTTCGCTGAATCAATTCAGGCAA38130.20865836447521574No Hit
TTTTGAGTGTAATAGAGATGGTAACATATATACAAATGAACTCCTTCACA35690.19530598028115526No Hit
TGTCATGTTGAGTAATTTTCCAATTAATGCCTTAGCATTCAAGCGAAGTG34060.1863861498564345No Hit
TTTTCTTTGCACTTTACCTCTATAGCAAACCTTGGAATCAATTTACTTGG32860.17981940353148673No Hit
CTTGTCAACTGTGATTGGACAAGAGTTAGCCGCTCCTAAGGGATAGCTGA28370.1552488276989738No Hit
GGCTGAACGCCTCTTAAGCCAGAAACCATGCTGATTAAACAATACTATCT28060.1535524182316956No Hit
TTCTTGTCAACTGTGATTGGACAAGAGTTAGCCGCTCCTAAGGGATAGCT27410.14999543063901555No Hit
GTTAAAACGCTCGTAGTTGAATTTCAAAGAATCGATATTTTAAGCAACGC26850.14693094902070658No Hit
TTTCTTTGCACTTTACCTCTATAGCAAACCTTGGAATCAATTTACTTGGA26360.14424952760468623No Hit
CTCGGTTTGTGCAAGCTTACCTTGGGAATTGTCATGTTGAGTAATTTTCC23600.12914601105730636No Hit
TTTACTATTATTTTCTTATACATATAGTATTGTGACAGTAATCCAACTTG22570.12350955379505951No Hit
TGTGCAAGTGTGTATGATATGTTAAACATTTGGTACTTCGTTGCCTTCTA22530.12329066225089458No Hit
GTGCAAGTGTGTATGATATGTTAAACATTTGGTACTTCGTTGCCTTCTAT22480.12301704782068842No Hit
CGCCTCTTAAGCCAGAAACCATGCTGATTAAACAATACTATCTCGTTCTT21970.12022618063258561No Hit
CCTTGTTACTTTGAGTAAATTAGAGTGTTCAAAGCAAACAGATATAGCAT21910.11989784331633822No Hit
GGAAAATCCCTCGGTTTGTGCAAGCTTACCTTGGGAATTGTCATGTTGAG21600.11820143384906004No Hit
GCTGAACGCCTCTTAAGCCAGAAACCATGCTGATTAAACAATACTATCTC21030.11508222934470985No Hit
CTTGGAGAAGAGGTTCGCTGAATCAATTCAGGCAAATTATTTTTTTGGGG20520.11229136215660704No Hit
GTCATGTTGAGTAATTTTCCAATTAATGCCTTAGCATTCAAGCGAAGTGA20360.11141579597994733No Hit
CTTGGAAGTCCTTGTTACTTTGAGTAAATTAGAGTGTTCAAAGCAAACAG20030.10960994074058669No Hit
TTTTGCTATTATGTGTTCTTTTAATTAAAATGATTCTTTTTAAGGACTTT19660.10758519395706115No Hit
TTTTGTAAGAAATATGCATTTCATCCCCGTCTCGAAAGGGAAACAGGTTG19190.10501321831312327No Hit
GTTTTGTAAGAAATATGCATTTCATCCCCGTCTCGAAAGGGAAACAGGTT19150.10479432676895833No Hit
GCTAAGTCTGTTGGATAATGGCTGAACGCCTCTTAAGCCAGAAACCATGC18990.10391876059229863No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)