FastQCFastQC Report
Thu 2 Nov 2023
HMLTGDMXY_n02_MHC-062.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHMLTGDMXY_n02_MHC-062.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2299280
Sequences flagged as poor quality0
Sequence length101
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GTTTCTTGTCAACTGTGATTGGACAAGAGTTAGCCGCTCCTAAGGGATAG314611.3682979019519153No Hit
GGTTTCTTGTCAACTGTGATTGGACAAGAGTTAGCCGCTCCTAAGGGATA213480.9284645628196652No Hit
GTTTTCTTTGCACTTTACCTCTATAGCAAACCTTGGAATCAATTTACTTG195160.8487874465049928No Hit
CTCAAAAGGAAAATCCCTCGGTTTGTGCAAGCTTACCTTGGGAATTGTCA150300.6536828920357677No Hit
GTCCTTGTTACTTTGAGTAAATTAGAGTGTTCAAAGCAAACAGATATAGC102220.44457395358547025No Hit
TTTCTTGTCAACTGTGATTGGACAAGAGTTAGCCGCTCCTAAGGGATAGC98280.4274381545527296No Hit
GCAAGCTTACCTTGGGAATTGTCATGTTGAGTAATTTTCCAATTAATGCC93590.4070404648411676No Hit
CCTCGGTTTGTGCAAGCTTACCTTGGGAATTGTCATGTTGAGTAATTTTC92800.403604606659476No Hit
GTTAGCCGCTCCTAAGGGATAGCTGAAAAGTGTTTATTTGGGGAATGTTT92330.40156048850074805No Hit
TGTGCAAGTGTGTATGAAATGTTAAACATTTGGTACTTCGCTACCTTCTA88780.38612087262099437No Hit
TGTGCAAGTGTGTATGATATGTTAAACATTTGGTACTTCGTTACCTTCTA85360.3712466511255697No Hit
GGGTTTCTTGTCAACTGTGATTGGACAAGAGTTAGCCGCTCCTAAGGGAT84790.36876761420966564No Hit
GTTCGCTGAATCAATTCAGGCAAATTATTTTTTTGGGGTTTCGGCCTTGA83860.36472286976792734No Hit
GTGCAAGTGTGTATGATATGTTAAACATTTGGTACTTCGTTACCTTCTAT79290.34484708256497687No Hit
CTTGGAATCAATTTACTTGGAGAAGAGGTTCGCTGAATCAATTCAGGCAA73250.31857798963153683No Hit
GTTGGTTCAGACAATTGGTACAGCAATTGGTTGACAAACCAGTGTTGCGA72300.3144462614383633No Hit
TTTTGAGTGTAATAGAGATGGTAACATATATACAAATGAACTCCTTCACA71720.3119237326467416No Hit
GTGCAAGTGTGTATGAAATGTTAAACATTTGGTACTTCGCTACCTTCTAT69070.3003983855815734No Hit
TGTCATGTTGAGTAATTTTCCAATTAATGCCTTAGCATTCAAGCGAAGTG68300.29704951115131695No Hit
TTTTCTTTGCACTTTACCTCTATAGCAAACCTTGGAATCAATTTACTTGG63230.2749991301624856No Hit
CTTGGAAGTCCTTGTTACTTTGAGTAAATTAGAGTGTTCAAAGCAAACAG59020.25668905048536933No Hit
TGCTGATTAAACAATACTATCTCGTTCTTTTTTGTTATCCTTTTACTGCT58640.2550363592080999No Hit
CCTTGTTACTTTGAGTAAATTAGAGTGTTCAAAGCAAACAGATATAGCAT53780.23389930760933855No Hit
CTTGTCAACTGTGATTGGACAAGAGTTAGCCGCTCCTAAGGGATAGCTGA48160.20945687345603842No Hit
TTTCTTTGCACTTTACCTCTATAGCAAACCTTGGAATCAATTTACTTGGA48080.2091089384502975No Hit
TTCTTGTCAACTGTGATTGGACAAGAGTTAGCCGCTCCTAAGGGATAGCT46030.20019310392818623No Hit
CTCGGTTTGTGCAAGCTTACCTTGGGAATTGTCATGTTGAGTAATTTTCC45270.19688772137364743No Hit
GTTAAAACGCTCGTAGTTGAATTTCAAAGAATCGATATTTTAAGCAACGC42880.1864931630771372No Hit
GTTTTGTAAGAAATATGCATTTCATCCCCGTCTCGAAAGGGAAACAGGTT42820.1862322118228315No Hit
GGAAAATCCCTCGGTTTGTGCAAGCTTACCTTGGGAATTGTCATGTTGAG42190.1834922236526217No Hit
CTTGGAGAAGAGGTTCGCTGAATCAATTCAGGCAAATTATTTTTTTGGGG40290.17522876726627468No Hit
GGTTGATATTCCTGTGCCGATAGTATTTTGAGTGTAATAGAGATGGTAAC40070.1742719460004871No Hit
GGCTGAACGCCTCTTAAGCCAGAAACCATGCTGATTAAACAATACTATCT39480.17170592533314777No Hit
TTTTGTAAGAAATATGCATTTCATCCCCGTCTCGAAAGGGAAACAGGTTG37780.16431230646115305No Hit
GTCATGTTGAGTAATTTTCCAATTAATGCCTTAGCATTCAAGCGAAGTGA37720.16405135520684735No Hit
TTTCGAAAGATCTCTCAGGATCGCTGGAGTTGACTAATATAATTTTACCA37190.1617462857938137No Hit
GTGTGTATGAAATGTTAAACATTTGGTACTTCGCTACCTTCTATACGCAC36730.15974565951080338No Hit
GTTCTTTTAATTAAAATGATTCTTTTTAAGGACTTTCTTTGCTTCGGCTT36630.15931074075362722No Hit
TTTTGCTATTATGTGTTCTTTTAATTAAAATGATTCTTTTTAAGGACTTT35310.15356981315890192No Hit
GCTCAAAAGGAAAATCCCTCGGTTTGTGCAAGCTTACCTTGGGAATTGTC35180.1530044187745729No Hit
ATTCAAGCGAAGTGAGCAGGAAAGCTCTCCCTAAGAGGGTGAAAGGCCCG35090.15261299189311436No Hit
CGCCTCTTAAGCCAGAAACCATGCTGATTAAACAATACTATCTCGTTCTT35010.15226505688737343No Hit
CGCTGAATCAATTCAGGCAAATTATTTTTTTGGGGTTTCGGCCTTGAATT34870.1516561706273268No Hit
GCTGAATCAATTCAGGCAAATTATTTTTTTGGGGTTTCGGCCTTGAATTC34810.1513952193730211No Hit
GTTTATTTGGGGAATGTTTTGTAAGAAATATGCATTTCATCCCCGTCTCG33550.1459152430326015No Hit
TGTTGAGTAATTTTCCAATTAATGCCTTAGCATTCAAGCGAAGTGAGCAG33360.1450888973939668No Hit
GGTTCGCTGAATCAATTCAGGCAAATTATTTTTTTGGGGTTTCGGCCTTG33050.14374064924672073No Hit
CCAGCTCAAAAGGAAAATCCCTCGGTTTGTGCAAGCTTACCTTGGGAATT32380.14082669357364044No Hit
GTGGAGAAGGGTTTCTTGTCAACTGTGATTGGACAAGAGTTAGCCGCTCC32220.14013082356215859No Hit
GCTTAATCCACATAACTGATACTTCGTATCGACTTTGTGCGCATTTTGCT31970.1390435266692182No Hit
GCTGAAAAGTGTTTATTTGGGGAATGTTTTGTAAGAAATATGCATTTCAT31880.13865209978775966No Hit
CTTTCTTTGCTTCGGCTTGGAAGTCCTTGTTACTTTGAGTAAATTAGAGT31330.13626004662329075No Hit
GTTGAGTAATTTTCCAATTAATGCCTTAGCATTCAAGCGAAGTGAGCAGG30910.1344333878431509No Hit
TGGGAATTGTCATGTTGAGTAATTTTCCAATTAATGCCTTAGCATTCAAG30550.13286768031731672No Hit
TTTCCAATTAATGCCTTAGCATTCAAGCGAAGTGAGCAGGAAAGCTCTCC30110.13095403778574163No Hit
GCTAAGTCTGTTGGATAATGGCTGAACGCCTCTTAAGCCAGAAACCATGC30020.1305626109042831No Hit
ATTTACTTGGAGAAGAGGTTCGCTGAATCAATTCAGGCAAATTATTTTTT29670.12904039525416652No Hit
CCTTCACATAGGTCTTACACTCGGGGTGCGTTTTCTTTGCACTTTACCTC29430.12799659023694374No Hit
TGTCAACTGTGATTGGACAAGAGTTAGCCGCTCCTAAGGGATAGCTGAAA29010.12616993145680389No Hit
CTGAAAAGTGTTTATTTGGGGAATGTTTTGTAAGAAATATGCATTTCATC28590.124343272676664No Hit
TTGGGAATTGTCATGTTGAGTAATTTTCCAATTAATGCCTTAGCATTCAA28300.12308200828085314No Hit
GCTGAACGCCTCTTAAGCCAGAAACCATGCTGATTAAACAATACTATCTC28040.12195121951219512No Hit
GTTGAATTTCAAAGAATCGATATTTTAAGCAACGCTTCTAGCTTAATCCA27250.11851536133050346No Hit
CCCGTCTCGAAAGGGAAACAGGTTGATATTCCTGTGCCGATAGTATTTTG26910.11703663755610451No Hit
CTTGGGAATTGTCATGTTGAGTAATTTTCCAATTAATGCCTTAGCATTCA25570.11120872620994399No Hit
CCTGTGCCGATAGTATTTTGAGTGTAATAGAGATGGTAACATATATACAA24720.10751191677394663No Hit
CTTGTTACTTTGAGTAAATTAGAGTGTTCAAAGCAAACAGATATAGCATT24320.105772241745242No Hit
CAGTAATCCAACTTGGTACGAGAGGATTAGTTGGTTCAGACAATTGGTAC24290.10564176611808913No Hit
CTTACCTTGGGAATTGTCATGTTGAGTAATTTTCCAATTAATGCCTTAGC24200.10525033923663059No Hit
CTGTGATTGGACAAGAGTTAGCCGCTCCTAAGGGATAGCTGAAAAGTGTT24140.1049893879823249No Hit
GTGTGTATGATATGTTAAACATTTGGTACTTCGTTACCTTCTATACGCAC23970.10425002609512543No Hit
GCTTACCTTGGGAATTGTCATGTTGAGTAATTTTCCAATTAATGCCTTAG23950.10416304234369021No Hit
TTTGTAAGAAATATGCATTTCATCCCCGTCTCGAAAGGGAAACAGGTTGA23940.1041195504679726No Hit
CTTGAATTCTTAATTTCCTGTTTTGTAGTAATAGTAATTCGTTTTTATGA23660.10290177794787933No Hit
GATTAGTTGGTTCAGACAATTGGTACAGCAATTGGTTGACAAACCAGTGT23280.10124908667060993No Hit
ATTTTTTTGGGGTTTCGGCCTTGAATTCTTAATTTCCTGTTTTGTAGTAA23270.10120559479489231No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)