FastQCFastQC Report
Thu 2 Nov 2023
HMLTGDMXY_n02_MHC-058.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHMLTGDMXY_n02_MHC-058.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences53253742
Sequences flagged as poor quality0
Sequence length101
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTTTCTTGTCAACTGTGATTGAACAAGAGTTAGCCGCTCCTAAGGGATAG3070910.576656190657926No Hit
GCTCAAACGGTTAATCCTTCGTTTGGTGCAAGCCTTTCGACGGAATTGTC2280750.4282797629507425No Hit
GTTTTCTTTGCACTTTACCTTTATAACAAACCTTGGAATCAATTTACTTG1275210.23945922898713862No Hit
CTTCGTTTGGTGCAAGCCTTTCGACGGAATTGTCGTTGAATTATTCCATG1145290.21506282131310137No Hit
GGTTTCTTGTCAACTGTGATTGAACAAGAGTTAGCCGCTCCTAAGGGATA1104970.20749152237977941No Hit
GTTGGTTCAGACAATTGGTACAGCAATTGGTTGACAAACCAGTGTTGCGA1039360.19517126139229804No Hit
GGCAAATCCGCCGAATTTAAGCATATAATTAAGCGGAAGAAAAGAAAATA928800.17441027899973677No Hit
CAGTAATCCAACTTGGTACGAGAGGATTAGTTGGTTCAGACAATTGGTAC908910.17067533019557574No Hit
CTCAAACGGTTAATCCTTCGTTTGGTGCAAGCCTTTCGACGGAATTGTCG902240.16942283605159617No Hit
GCTAAGTCTGTTGGATAATGGCTGAACGCCTCTTAAGCCAGAAACCATGC860120.1615135327016081No Hit
CGGCGAGTGAACAAGGACCAGCTCAAACGGTTAATCCTTCGTTTGGTGCA845310.15873250747337153No Hit
GGGTTTCTTGTCAACTGTGATTGAACAAGAGTTAGCCGCTCCTAAGGGAT802920.15077250346088356No Hit
CAAGGACCAGCTCAAACGGTTAATCCTTCGTTTGGTGCAAGCCTTTCGAC752440.1412933573757127No Hit
GCAAGCCTTTCGACGGAATTGTCGTTGAATTATTCCATGAATGCCTTTAA696000.13069504111091385No Hit
GATTAGTTGGTTCAGACAATTGGTACAGCAATTGGTTGACAAACCAGTGT660220.1239762644285166No Hit
GTCTACTTGACATTTTTATTATAAGGATAACTACGGAAAAGCTGTAGCTA643630.12086099038824351No Hit
GGAGTCTAGCAAATGTGCAAGTGTATATGATTCTTTAAACATTTCTCTTT634170.11908458939843138No Hit
TTTCTTGTCAACTGTGATTGAACAAGAGTTAGCCGCTCCTAAGGGATAGC630790.11844989221602494No Hit
GGTTAATCCTTCGTTTGGTGCAAGCCTTTCGACGGAATTGTCGTTGAATT629960.11829403462389554No Hit
AGGGTTTCTTGTCAACTGTGATTGAACAAGAGTTAGCCGCTCCTAAGGGA628690.11805555372991441No Hit
GTTGAATTATTCCATGAATGCCTTTAATTTGAGCAAAGTGAGCAGGAATG628290.11798044163732194No Hit
GGGAAATCGTTTTCCTTGACCTATTCTCAAACTCCCAATAGGTAAAAAGG623250.1170340292706567No Hit
GTGGAGAAGGGTTTCTTGTCAACTGTGATTGAACAAGAGTTAGCCGCTCC621460.11669790265630535No Hit
GTTTGACTGGGGCGGTACATCTGTTAAATATTAACGCAGATGTCCAAAGA601250.11290286417807034No Hit
CGGAATTGTCGTTGAATTATTCCATGAATGCCTTTAATTTGAGCAAAGTG600510.11276390680677426No Hit
GGCTGAACGCCTCTTAAGCCAGAAACCATGCTGATTAAACAATACTATTT572080.1074253148257638No Hit
ATTTACTTGGAGAAGAGGTTCGTTGAACTCAATTCAAAAAATTTCTTGAA568450.10674367258548705No Hit
GTTCATTTTATTTTTTGTTTTGTAGCAATAGTAATTCGTTTTTATGAATT560690.10528649798919294No Hit
GTGCAAGTGTATATGATTCTTTAAACATTTCTCTTTTTAATATACGCATA549610.10320589302438127No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)