FastQCFastQC Report
Thu 2 Nov 2023
HMLTGDMXY_n02_MHC-057.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHMLTGDMXY_n02_MHC-057.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1260159
Sequences flagged as poor quality0
Sequence length101
%GC41

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTCAAAAGGAAAATCCCTCGGTTTGTGCAAGCTTACCTTGGGAATTGTCA200931.594481331324063No Hit
GGAAAATCCCTCGGTTTGTGCAAGCTTACCTTGGGAATTGTCATGTTGAG118420.9397226857880633No Hit
GTTTCTTGTCAACTGTGATTGGACAAGAGTTAGCCGCTCCTAAGGGATAG118370.9393259104605053No Hit
GTTTTCTTTGCACTTTACCTCTATAGCAAACCTTGGAATCAATTTACTTG94520.7500640792154006No Hit
CCTCGGTTTGTGCAAGCTTACCTTGGGAATTGTCATGTTGAGTAATTTTC68110.5404873511993328No Hit
GCAAGCTTACCTTGGGAATTGTCATGTTGAGTAATTTTCCAATTAATGCC67840.5383447644305203No Hit
GTTAGCCGCTCCTAAGGGATAGCTGAAAAGTGTTTATTTGGGGAATGTTT65330.5184266429871152No Hit
GGTTTCTTGTCAACTGTGATTGGACAAGAGTTAGCCGCTCCTAAGGGATA62940.4994607823298488No Hit
TGCTGATTAAACAATACTATCTCGTTCTTTTTTGTTATCCTTTTACTGCT49160.3901095020548994No Hit
GTCCTTGTTACTTTGAGTAAATTAGAGTGTTCAAAGCAAACAGATATAGC37780.29980343750272787No Hit
GGTTGATATTCCTGTGCCGATAGTATTTTGAGTGTAATAGAGATGGTAAC37630.2986131115200542No Hit
GTGCAAGTGTGTATGATATGTTAAACATTTGGTACTTCGCTACCTTCTAT37470.29734343047186906No Hit
GGGTTTCTTGTCAACTGTGATTGGACAAGAGTTAGCCGCTCCTAAGGGAT36890.29274083667219775No Hit
GCTTAATCCACATAACTGATACTTCGTATCGACTTTGTGCGCATTTTGCT36380.2886937283311074No Hit
GCTAAGTCTGTTGGATAATGGCTGAACGCCTCTTAAGCCAGAAACCATGC35630.28274209841773934No Hit
GTTGGTTCAGACAATTGGTACAGCAATTGGTTGACAAACCAGTGTTGCGA35550.2821072578936467No Hit
GTGCAAGTGTGTATGATATGTTAAACATTTGGTACTTCGTTACCTTCTAT33470.26560140426723927No Hit
GTTGAATTTCAAAGAATCGACATTTTAAGCAACGCTTCTAGCTTAATCCA32040.2542536298990842No Hit
GGCTGAACGCCTCTTAAGCCAGAAACCATGCTGATTAAACAATACTATCT31500.24996845636145915No Hit
CTGAAAAGTGTTTATTTGGGGAATGTTTTGTAAGAAATATGCATTTCATC31370.24893684050980866No Hit
CTCGGTTTGTGCAAGCTTACCTTGGGAATTGTCATGTTGAGTAATTTTCC30760.24409618151360266No Hit
GCTGAAAAGTGTTTATTTGGGGAATGTTTTGTAAGAAATATGCATTTCAT30650.2432232757929753No Hit
CTTGGAATCAATTTACTTGGAGAAGAGGTTCGCTGAATCAATTCAGGCAA30220.23981100797597762No Hit
GTTTTGTAAGAAATATGCATTTCATCCCCGTCTCGAAAGGGAAACAGGTT30190.23957294277944294No Hit
TTTCTTGTCAACTGTGATTGGACAAGAGTTAGCCGCTCCTAAGGGATAGC30100.23885874718983874No Hit
GTCATGTTGAGTAATTTTCCAATTAATGCCTTAGCATTCAAGCGAAGTGA30080.2387000370588156No Hit
GTTGAATTTCAAAGAATCGATATTTTAAGCAACGCTTCTAGCTTAATCCA28930.22957420452498456No Hit
GTTCTTTTAATTAAAATGATTCTTTTTAAGGACTTTCTTTGCTTCGGCTT28720.2279077481492415No Hit
TTTTGAGTGTAATAGAGATGGTAACATATATACAAATGAACTCCTTCACA28620.22711419749412576No Hit
TTTCGAAAGATCTCTCAGGATCGCTGGAGTTGACTAATATAATTTTACCA27850.22100385744973453No Hit
CAGCGAACTTGGATAATTATGATAAAACAAATCACTGAAGTTGTTGGGAA26950.2138619015536928No Hit
TCAAAAGGAAAATCCCTCGGTTTGTGCAAGCTTACCTTGGGAATTGTCAT26210.20798962670583632No Hit
GTGGAGAAGGGTTTCTTGTCAACTGTGATTGGACAAGAGTTAGCCGCTCC25990.2062438152645817No Hit
GTTCGCTGAATCAATTCAGGCAAATTATTTTTTTGGGGTTTCGGCCTTGA25770.20449800382332708No Hit
GCTGATTAAACAATACTATCTCGTTCTTTTTTGTTATCCTTTTACTGCTG25670.20370445316821134No Hit
GTTAAAACGCTCGTAGTTGAATTTCAAAGAATCGACATTTTAAGCAACGC25110.19926056949956314No Hit
GCTGAACGCCTCTTAAGCCAGAAACCATGCTGATTAAACAATACTATCTC25060.19886379417200528No Hit
CAGCGAACTTGGATAATTATGATAAAACAATTCACTGAAGTTGTTGGGAA24130.19148377307942888No Hit
CAAGAGTTAGCCGCTCCTAAGGGATAGCTGAAAAGTGTTTATTTGGGGAA23580.18711924447629227No Hit
CTTGGAAGTCCTTGTTACTTTGAGTAAATTAGAGTGTTCAAAGCAAACAG23560.18696053434526913No Hit
GCTCAAAAGGAAAATCCCTCGGTTTGTGCAAGCTTACCTTGGGAATTGTC23540.186801824214246No Hit
GGGAAACAGGTTGATATTCCTGTGCCGATAGTATTTTGAGTGTAATAGAG23000.18251665067662098No Hit
TTTTAAGCAACGCTTCTAGCTTAATCCACATAACTGATACTTCGTATCGA22550.17894567272860012No Hit
GTTAAAACGCTCGTAGTTGAATTTCAAAGAATCGATATTTTAAGCAACGC22520.1787076075320654No Hit
GTTTATTTGGGGAATGTTTTGTAAGAAATATGCATTTCATCCCCGTCTCG22300.17696179609081075No Hit
TTTCTTTGCACTTTACCTCTATAGCAAACCTTGGAATCAATTTACTTGGA22080.17521598464955612No Hit
CGCCTCTTAAGCCAGAAACCATGCTGATTAAACAATACTATCTCGTTCTT21950.17418436879790566No Hit
GTTGAGTAATTTTCCAATTAATGCCTTAGCATTCAAGCGAAGTGAGCAGG21780.1728353326842089No Hit
TGGGAATTGTCATGTTGAGTAATTTTCCAATTAATGCCTTAGCATTCAAG21490.17053403578437323No Hit
CTGATACTTCGTATCGACTTTGTGCGCATTTTGCTATTATGTGTTCTTTT21470.1703753256533501No Hit
TGTCATGTTGAGTAATTTTCCAATTAATGCCTTAGCATTCAAGCGAAGTG21380.16966113006374592No Hit
TTTTGCTATTATGTGTTCTTTTAATTAAAATGATTCTTTTTAAGGACTTT20910.16593144198470194No Hit
CAGTAATCCAACTTGGTACGAGAGGATTAGTTGGTTCAGACAATTGGTAC20860.16553466665714406No Hit
TTTTCTTTGCACTTTACCTCTATAGCAAACCTTGGAATCAATTTACTTGG20430.16212239884014634No Hit
CAAAAGGAAAATCCCTCGGTTTGTGCAAGCTTACCTTGGGAATTGTCATG20400.16188433364361163No Hit
AGCAAACCTTGGAATCAATTTACTTGGAGAAGAGGTTCGCTGAATCAATT19730.15656754425433617No Hit
ATTTACTTGGAGAAGAGGTTCGCTGAATCAATTCAGGCAAATTATTTTTT19720.1564881891888246No Hit
GAGAAATTATACGTGCTTCCTCTACGGATGCATTCATAATCCTATCACCT19460.15442495748552365No Hit
CGCGTGCTACACTGATATGTATAACGAGTTATTAAAATTACGATTCAGCT18920.15013978394789865No Hit
GCTTACCTTGGGAATTGTCATGTTGAGTAATTTTCCAATTAATGCCTTAG18900.1499810738168755No Hit
GAGAAATTATACATGCTTCCTCTACGGATGCATTCATAATCCTATCACCT18760.14887010289971345No Hit
CTTGTCAACTGTGATTGGACAAGAGTTAGCCGCTCCTAAGGGATAGCTGA18250.14482299455862316No Hit
GTTGGATAATGGCTGAACGCCTCTTAAGCCAGAAACCATGCTGATTAAAC18230.14466428442760002No Hit
CGGAAAAGCTGTAGCTAATACTTGCTTTAGCACTCTTGATTCATTTCTTG17890.14196621220020647No Hit
CTAGGCTGCACGCGTGCTACACTGATATGTATAACGAGTTATTAAAATTA17690.140379110889975No Hit
GCTCGTAGTTGAATTTCAAAGAATCGACATTTTAAGCAACGCTTCTAGCT17130.13593522722132684No Hit
TGGAGAAGGGTTTCTTGTCAACTGTGATTGGACAAGAGTTAGCCGCTCCT17070.13545909682825738No Hit
ATTTTAAGCAACGCTTCTAGCTTAATCCACATAACTGATACTTCGTATCG16990.1348242563041648No Hit
CTTACCTTGGGAATTGTCATGTTGAGTAATTTTCCAATTAATGCCTTAGC16980.13474490123865324No Hit
CGAAAGGGAAACAGGTTGATATTCCTGTGCCGATAGTATTTTGAGTGTAA16980.13474490123865324No Hit
CCTTGTTACTTTGAGTAAATTAGAGTGTTCAAAGCAAACAGATATAGCAT16920.13426877084558378No Hit
TTCTTGTCAACTGTGATTGGACAAGAGTTAGCCGCTCCTAAGGGATAGCT16920.13426877084558378No Hit
GTTGATATTCCTGTGCCGATAGTATTTTGAGTGTAATAGAGATGGTAACA16920.13426877084558378No Hit
TTTGTAAGAAATATGCATTTCATCCCCGTCTCGAAAGGGAAACAGGTTGA16580.13157069861819023No Hit
TTTCAAAGAATCGACATTTTAAGCAACGCTTCTAGCTTAATCCACATAAC16320.12950746691488932No Hit
CCAGCTCAAAAGGAAAATCCCTCGGTTTGTGCAAGCTTACCTTGGGAATT16320.12950746691488932No Hit
GAAAATCCCTCGGTTTGTGCAAGCTTACCTTGGGAATTGTCATGTTGAGT16110.12784101053914626No Hit
CGCTCGTAGTTGAATTTCAAAGAATCGACATTTTAAGCAACGCTTCTAGC16090.12768230040812312No Hit
CCTTCACATAGGTCTTACACTCGGGGTGCGTTTTCTTTGCACTTTACCTC15960.12665068455647266No Hit
TTTTGTAAGAAATATGCATTTCATCCCCGTCTCGAAAGGGAAACAGGTTG15850.12577777883584532No Hit
ATTCTTTTTAAGGACTTTCTTTGCTTCGGCTTGGAAGTCCTTGTTACTTT15360.12188938062577817No Hit
TGTGCAAGTGTGTATGATATGTTAAACATTTGGTACTTCGCTACCTTCTA15330.12165131542924346No Hit
CTTGGAGAAGAGGTTCGCTGAATCAATTCAGGCAAATTATTTTTTTGGGG15310.12149260529822031No Hit
GCTCGTAGTTGAATTTCAAAGAATCGATATTTTAAGCAACGCTTCTAGCT15270.12117518503617401No Hit
GATTAGTTGGTTCAGACAATTGGTACAGCAATTGGTTGACAAACCAGTGT15130.12006421411901197No Hit
CCAGAAACCATGCTGATTAAACAATACTATCTCGTTCTTTTTTGTTATCC14890.1181596925467342No Hit
CTCGGGGTGCGTTTTCTTTGCACTTTACCTCTATAGCAAACCTTGGAATC14740.11696936656406057No Hit
CGACATTTTAAGCAACGCTTCTAGCTTAATCCACATAACTGATACTTCGT14640.11617581590894481No Hit
TGTGCAAGTGTGTATGATATGTTAAACATTTGGTACTTCGTTACCTTCTA14590.11577904058138697No Hit
CTTTTTAAGGACTTTCTTTGCTTCGGCTTGGAAGTCCTTGTTACTTTGAG14400.11427129433666704No Hit
GGAACATCAGCGAACTTGGATAATTATGATAAAACAAATCACTGAAGTTG14210.11276354809194712No Hit
CTTACACTCGGGGTGCGTTTTCTTTGCACTTTACCTCTATAGCAAACCTT14190.11260483796092398No Hit
TTTCAAAGAATCGATATTTTAAGCAACGCTTCTAGCTTAATCCACATAAC14160.11236677276438925No Hit
AGGGTTTCTTGTCAACTGTGATTGGACAAGAGTTAGCCGCTCCTAAGGGA14120.11204935250234296No Hit
TTGGGAATTGTCATGTTGAGTAATTTTCCAATTAATGCCTTAGCATTCAA14080.11173193224029666No Hit
TTTTAATTAAAATGATTCTTTTTAAGGACTTTCTTTGCTTCGGCTTGGAA14060.11157322210927352No Hit
CCCTCGGTTTGTGCAAGCTTACCTTGGGAATTGTCATGTTGAGTAATTTT13980.1109383815851809No Hit
GGAGAAGGGTTTCTTGTCAACTGTGATTGGACAAGAGTTAGCCGCTCCTA13950.11070031638864619No Hit
AGCGAACTTGGATAATTATGATAAAACAAATCACTGAAGTTGTTGGGAAT13850.10990676573353045No Hit
GGAACATCAGCGAACTTGGATAATTATGATAAAACAATTCACTGAAGTTG13510.10720869350613692No Hit
CTTTCTTTGCTTCGGCTTGGAAGTCCTTGTTACTTTGAGTAAATTAGAGT13430.10657385298204433No Hit
CAGCAATTGGTTGACAAACCAGTGTTGCGAAGCTAAGTCTGTTGGATAAT13260.10522481686834756No Hit
CGCTCGTAGTTGAATTTCAAAGAATCGATATTTTAAGCAACGCTTCTAGC13090.10387578075465079No Hit
TTTTTAAGGACTTTCTTTGCTTCGGCTTGGAAGTCCTTGTTACTTTGAGT12960.10284416490300033No Hit
GTTACTTTGAGTAAATTAGAGTGTTCAAAGCAAACAGATATAGCATTGCG12700.1007809331996994No Hit
CGCTGAATCAATTCAGGCAAATTATTTTTTTGGGGTTTCGGCCTTGAATT12620.10014609267560681No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)