FastQCFastQC Report
Thu 2 Nov 2023
HMLTGDMXY_n02_MHC-048.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHMLTGDMXY_n02_MHC-048.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences48811975
Sequences flagged as poor quality0
Sequence length101
%GC39

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTTTCTTGTCAACTGTGATTGGACAAGAGTTAGCCGCTCCTAAGGGATAG3564590.7302695701208566No Hit
GGAAAATCCCTCGGTTTGTGCAAGCTTACCTTGGGAATTGTCATGTTGAG2479460.5079614172546798No Hit
CTCAAAAGGAAAATCCCTCGGTTTGTGCAAGCTTACCTTGGGAATTGTCA2400420.49176866947096487No Hit
GTTTGACTGGGGCGGTACATCTGTTAAACAATAACGCAGATGTCCAAAGA1566530.32093149273308447No Hit
CTTTACTCTAGTCTGGCTTTGTGAAACGACTTAAGAGGTGTAGCATAAGT1561780.31995837087108236No Hit
CCCAATTCTAAAGAAGAGAGGTAGTGACAAGAAATAACAATACAAGGCCA1498850.30706604270775767No Hit
GTTTTCTTTGCACTTTACCTCTATAGCAAACCTTGGAATCAATTTACTTG1413680.28961745555265894No Hit
AAACGACTTAAGAGGTGTAGCATAAGTGGGAGTAGAGACTGAAATATGTT1385490.28384223338637704No Hit
AGAAGACCCTGTTGAGCTTTACTCTAGTCTGGCTTTGTGAAACGACTTAA1365400.2797264400795092No Hit
TATTACTCGGTTCGCCGGGTATTCATATTAATTGAATTAATAAGTGGCAC1259550.25804118763889394No Hit
CGCACAATTAATGTAATATGTTAATTATTGTAGAACTGGCTGGAAGGCAA1250020.2560887978820771No Hit
GTTAGCCGCTCCTAAGGGATAGCTGAAAAGTGTTTATTTGGGGAATGTTT1229340.2518521325965606No Hit
CGACTTAAGAGGTGTAGCATAAGTGGGAGTAGAGACTGAAATATGTTTTT1170310.2397587886988797No Hit
CTTGTCAACTGTGATTGGACAAGAGTTAGCCGCTCCTAAGGGATAGCTGA1157880.23721228243684056No Hit
GTTCAAAGCAAACAGATATAGCATTGCGCGTTTGAATACTACAGCATGGA1115650.22856071691424082No Hit
CTTGTTAAGCCTTTTAAGAAAAAAGTTATTAACTTAAGGAATTATAACAA1106380.22666159277513354No Hit
CGGGTATTCATATTAATTGAATTAATAAGTGGCACTTCTCATAGTGTTTC1101030.22556555025687858No Hit
CAAGAGTTAGCCGCTCCTAAGGGATAGCTGAAAAGTGTTTATTTGGGGAA1090030.22331200489224212No Hit
GGGAAATCGTTTTCCTTGACCTATTCTCAAACTCCCAATAGGTAAAAAGG1076620.22056472822498985No Hit
AGCAAACCTTGGAATCAATTTACTTGGAGAAGAGGTTCGCTGAATCAATT1031600.21134158165081418No Hit
GTCATGTTGAGTAATTTTCCAATTAATGCCTTAGCATTCAAGCGAAGTGA1029990.21101174455653557No Hit
AACGACTTAAGAGGTGTAGCATAAGTGGGAGTAGAGACTGAAATATGTTT1002390.20535739436890232No Hit
GTAACAACTCACCTACCGAATGAACTAGCCCTGAAAATGGATGGCGCTAA987440.20229462135060097No Hit
CTGAAATATGTTTTTACGACAGTGAAATACCACTACTTTTAAAGTTGTTT958300.1963247748119186No Hit
AATGGATCCGTCCATTTTTAGTGTGTATCAATCGAGTTTCTGACCTATCA945310.19366354260404336No Hit
CGGAAAAGCTGTAGCTAATACTTGCTTTAGCACTCTTGATTCATTTCTTG922440.1889782169232038No Hit
GTTAAACATTTGGTACTTCGTTACCTTCTATACGCACAATTAATGTAATA920360.18855209198152706No Hit
CCTCGGTTTGTGCAAGCTTACCTTGGGAATTGTCATGTTGAGTAATTTTC884830.18127314045375137No Hit
TTTCTTGTCAACTGTGATTGGACAAGAGTTAGCCGCTCCTAAGGGATAGC878230.17992101323496948No Hit
CTTGAATTCTTAATTTCCTGTTTTGTAGTAATAGTAATTCGTTTTTATGA867380.17769819803439627No Hit
GTCCTTGTTACTTTGAGTAAATTAGAGTGTTCAAAGCAAACAGATATAGC854400.1750390145041253No Hit
TGTGAAACGACTTAAGAGGTGTAGCATAAGTGGGAGTAGAGACTGAAATA853440.17484234145412064No Hit
GAACTAGGCTGCACGCGTGCTACACTGATATGTATAACGAGTTATTAAAA847160.17355577191867363No Hit
GGTTTCTTGTCAACTGTGATTGGACAAGAGTTAGCCGCTCCTAAGGGATA825310.169079411353464No Hit
GGAGTCTAGCAAATGTGCAAGTGTGTATGATATGTTAAACATTTGGTACT800020.16389830569240438No Hit
GTTACTTTGAGTAAATTAGAGTGTTCAAAGCAAACAGATATAGCATTGCG789500.16174309685277025No Hit
GGAAAAGCTGTAGCTAATACTTGCTTTAGCACTCTTGATTCATTTCTTGA786860.1612022459652575No Hit
CTAGGCTGCACGCGTGCTACACTGATATGTATAACGAGTTATTAAAATTA759880.15567491378908557No Hit
GTTAAACATTTGGTACTTCGCTACCTTCTATACGCACAATTAATGTAATA753430.15435351673436692No Hit
CTCGGTTCGCCGGGTATTCATATTAATTGAATTAATAAGTGGCACTTCTC741200.15184798402441205No Hit
GTCTAGCAAATGTGCAAGTGTGTATGATATGTTAAACATTTGGTACTTCG717370.1469659852935678No Hit
CATAATCCTATCACCTTTTTCGTGAGCTGCTGTTCGTGAGCTGTGAGCTG714070.1462899216841769No Hit
GGGAAATATATTACTCGGTTCGCCGGGTATTCATATTAATTGAATTAATA707610.14496647595185402No Hit
AGCAAACAGATATAGCATTGCGCGTTTGAATACTACAGCATGGAATAACA701610.14373726938932505No Hit
GAAGACCCTGTTGAGCTTTACTCTAGTCTGGCTTTGTGAAACGACTTAAG697210.14283585124347048No Hit
CTTGGAGAAGAGGTTCGCTGAATCAATTCAGGCAAATTATTTTTTTGGGG693050.14198360136011706No Hit
ATTTACTTGGAGAAGAGGTTCGCTGAATCAATTCAGGCAAATTATTTTTT690700.14150216212312655No Hit
GCAAGCTTACCTTGGGAATTGTCATGTTGAGTAATTTTCCAATTAATGCC683450.1400168708600707No Hit
GGAAAGCTCTCCCTAAGAGGGTGAAAGGCCCGTAGTTTGTTGCTTTCTAT672310.13773464400897525No Hit
GTTTATTTGGGGAATGTTTTGTAAGAAATATGCATTTCATCCCCGTCTCG669220.13710160262927282No Hit
GAAATATATTACTCGGTTCGCCGGGTATTCATATTAATTGAATTAATAAG668290.13691107561208085No Hit
GTAAGAAATATGCATTTCATCCCCGTCTCGAAAGGGAAACAGGTTGATAT664300.13609365324799907No Hit
CTGAAAAGTGTTTATTTGGGGAATGTTTTGTAAGAAATATGCATTTCATC662780.13578225425215842No Hit
CAAAAGGAAAATCCCTCGGTTTGTGCAAGCTTACCTTGGGAATTGTCATG660830.1353827621193365No Hit
CTTGGAATCAATTTACTTGGAGAAGAGGTTCGCTGAATCAATTCAGGCAA655820.1343563746396248No Hit
GTTTTACTTATCTATTGATGGGGAAATATATTACTCGGTTCGCCGGGTAT646680.13248388330937233No Hit
GCACAATTAATGTAATATGTTAATTATTGTAGAACTGGCTGGAAGGCAAC646190.1323834981067658No Hit
CAAGGACCAGCTCAAAAGGAAAATCCCTCGGTTTGTGCAAGCTTACCTTG645690.13228106422655506No Hit
CTCTAGTCTGGCTTTGTGAAACGACTTAAGAGGTGTAGCATAAGTGGGAG644440.1320249795260282No Hit
GTGAAAGTATATGCATATTTTATATGTAGAAACTGCGAACGGCTCATTAA634850.13006029770358604No Hit
ATTAAATAGTACCTGAAATCGTTAAGATGGAACGGATTAAGAGAGAAAAA615080.1260100620800531No Hit
TAACAACTCACCTACCGAATGAACTAGCCCTGAAAATGGATGGCGCTAAA610860.12514552013107438No Hit
CTTAAGAGGTGTAGCATAAGTGGGAGTAGAGACTGAAATATGTTTTTACG603260.12358852515187103No Hit
AGACCCTGTTGAGCTTTACTCTAGTCTGGCTTTGTGAAACGACTTAAGAG600900.12310503723727631No Hit
CCCTGTTGAGCTTTACTCTAGTCTGGCTTTGTGAAACGACTTAAGAGGTG592550.12139439143775681No Hit
GTTACCTTCTATACGCACAATTAATGTAATATGTTAATTATTGTAGAACT588530.1205708230408624No Hit
CGGCGAGTGAACAAGGACCAGCTCAAAAGGAAAATCCCTCGGTTTGTGCA583530.11954648423875493No Hit
GAAAGCTCTCCCTAAGAGGGTGAAAGGCCCGTAGTTTGTTGCTTTCTATT575030.11780510827517222No Hit
CTTAGATGAACTAGGCTGCACGCGTGCTACACTGATATGTATAACGAGTT562630.11526474804594569No Hit
GACCCTGTTGAGCTTTACTCTAGTCTGGCTTTGTGAAACGACTTAAGAGG559270.11457639237092948No Hit
GAGAAATTATACGTGCTTCCTCTACGGATGCATTCATAATCCTATCACCT551420.11296818045162073No Hit
ATTTTTTTGGGGTTTCGGCCTTGAATTCTTAATTTCCTGTTTTGTAGTAA551110.11290467144589007No Hit
CTTGAGTGTGTACTTGTTAAGCCTTTTAAGAAAAAAGTTATTAACTTAAG550630.11280633492088776No Hit
GGACATTAGAATAGAGAAGAAAAGAAAATGGTTGAAAATAAAGGTATCGA543870.11142142886043845No Hit
GTTTAAGGCAACAACAGGTCTGTGATGTCCTTAGATGAACTAGGCTGCAC540060.11064088269323255No Hit
GCATAAGTGGGAGTAGAGACTGAAATATGTTTTTACGACAGTGAAATACC538290.11027826675728651No Hit
GTTCGCTGAATCAATTCAGGCAAATTATTTTTTTGGGGTTTCGGCCTTGA537150.110044717510406No Hit
GGAGAAGGGTTTCTTGTCAACTGTGATTGGACAAGAGTTAGCCGCTCCTA535000.1096042518254998No Hit
AAACGACTTAAGAGGTGTAGCATAAGTGGGAGTAGAAACTGAAATATGTT534690.10954074281976914No Hit
ATTCAAGCGAAGTGAGCAGGAAAGCTCTCCCTAAGAGGGTGAAAGGCCCG530980.10878068342860539No Hit
GAACTATCCTTGAAACAAGGAGGGAACAGCAATTAAAGGGGGATGATTTA527320.10803086742546271No Hit
TTCTTGTCAACTGTGATTGGACAAGAGTTAGCCGCTCCTAAGGGATAGCT525620.10768259223274616No Hit
GTTGAGTAATTTTCCAATTAATGCCTTAGCATTCAAGCGAAGTGAGCAGG524200.10739168001294765No Hit
CTAGCAAATGTGCAAGTGTGTATGATATGTTAAACATTTGGTACTTCGTT522700.1070843783723154No Hit
CTTAATTTCCTGTTTTGTAGTAATAGTAATTCGTTTTTATGAATTATCCG521160.1067688820212663No Hit
GAAAATCCCTCGGTTTGTGCAAGCTTACCTTGGGAATTGTCATGTTGAGT515290.10556630826759213No Hit
AGCGAAGTGAGCAGGAAAGCTCTCCCTAAGAGGGTGAAAGGCCCGTAGTT514960.10549870190665303No Hit
CGTAGTTTGTTGCTTTCTATTTTTTGGAATTTTACTTCTAGAGTCGTGTT512790.10505413886653839No Hit
CACTGATATGTATAACGAGTTATTAAAATTACGATTCAGCTTGCTGTTTC512760.10504799283372573No Hit
CCTTAGATGAACTAGGCTGCACGCGTGCTACACTGATATGTATAACGAGT512510.10499677589362037No Hit
GTGGAGAAGGGTTTCTTGTCAACTGTGATTGGACAAGAGTTAGCCGCTCC511000.10468742557538391No Hit
GTGCAAGTGTGTATGATATGTTAAACATTTGGTACTTCGTTACCTTCTAT509670.10441495145402332No Hit
GTAATATGTTAATTATTGTAGAACTGGCTGGAAGGCAACTTCCTGTCGGC507570.10398472915713819No Hit
CTGATATGTATAACGAGTTATTAAAATTACGATTCAGCTTGCTGTTTCGT504410.10333734703420626No Hit
GTCTAACACAAGGAAGTTTAAGGCAACAACAGGTCTGTGATGTCCTTAGA502800.10300750993992765No Hit
AGCATAAGTGGGAGTAGAGACTGAAATATGTTTTTACGACAGTGAAATAC501190.10267767284564905No Hit
CGCTGAATCAATTCAGGCAAATTATTTTTTTGGGGTTTCGGCCTTGAATT497760.1019749764274033No Hit
CTTGGGGGCATTTGTATTCAGATGTCAGAGGTGAAATTCTTAGATTTTCT494570.10132144827165876No Hit
GGCAACTTCCTGTCGGCGCATACAATACCGGTAAGCAATTACGCTATATT493920.10118828422738478No Hit
GGAGACATAGCCAGGTGGGGAGTTTGACTGGGGCGGTACATCTGTTAAAC493740.1011514080305089No Hit
GCCATTAAATAGTACCTGAAATCGTTAAGATGGAACGGATTAAGAGAGAA491280.10064743333987203No Hit
GTAGAACTGGCTGGAAGGCAACTTCCTGTCGGCGCATACAATACCGGTAA491080.10060645978778773No Hit

[WARN]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)