FastQCFastQC Report
Thu 2 Nov 2023
HMLTGDMXY_n02_MHC-046.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHMLTGDMXY_n02_MHC-046.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences39874068
Sequences flagged as poor quality0
Sequence length101
%GC39

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTTGGTTCAGACAATTGGTACAGCAATTGGTTGACAAACCAGTGTTGCGA3887410.974921846449176No Hit
CAGTAATCCAACTTGGTACGAGAGGATTAGTTGGTTCAGACAATTGGTAC2564170.6430670680503429No Hit
GTTTCTTGTCAACTGTGATTGAACAAGAGTTAGCCGCTCCTAAGGGATAG1885810.4729414616035665No Hit
GCTAAGTCTGTTGGATAATGGCTGAACGCCTCTTAAGCCAGAAACCATGC1759620.4412943269294721No Hit
GACAATTGGTACAGCAATTGGTTGACAAACCAGTGTTGCGAAGCTAAGTC1615910.4052533591506139No Hit
GATTAGTTGGTTCAGACAATTGGTACAGCAATTGGTTGACAAACCAGTGT1576860.3954600268023819No Hit
GGATTAGTTGGTTCAGACAATTGGTACAGCAATTGGTTGACAAACCAGTG1192490.29906404332760833No Hit
GAGGATTAGTTGGTTCAGACAATTGGTACAGCAATTGGTTGACAAACCAG1074440.2694583356782157No Hit
GTTTCTTGTCAACTGTGATTGGACAAGAGTTAGCCGCTCCTAAGGGATAG1052090.26385318899491267No Hit
GTGACAGTAATCCAACTTGGTACGAGAGGATTAGTTGGTTCAGACAATTG927630.23263992026095756No Hit
AATTGGTACAGCAATTGGTTGACAAACCAGTGTTGCGAAGCTAAGTCTGT911900.22869500046997965No Hit
AACCAGTGTTGCGAAGCTAAGTCTGTTGGATAATGGCTGAACGCCTCTTA906330.2272981026164674No Hit
GGAAAATCCCTCGGTTTGTGCAAGCTTACCTTGGGAATTGTCATGTTGAG837370.2100036545054796No Hit
GTTGAACTCAATTCAAAAAATTTCTTGAACGGGGTTTTCGGATTCAGTTC816940.204880023778863No Hit
AGCTAAGTCTGTTGGATAATGGCTGAACGCCTCTTAAGCCAGAAACCATG787240.19743157382386972No Hit
GTTCGTTGAACTCAATTCAAAAAATTTCTTGAACGGGGTTTTCGGATTCA778420.19521960989784137No Hit
CAAACCAGTGTTGCGAAGCTAAGTCTGTTGGATAATGGCTGAACGCCTCT770210.19316062760388533No Hit
CAATTGGTACAGCAATTGGTTGACAAACCAGTGTTGCGAAGCTAAGTCTG769520.19298758280695114No Hit
CTCAAAAGGAAAATCCCTCGGTTTGTGCAAGCTTACCTTGGGAATTGTCA757810.19005083705028541No Hit
ATCCAACTTGGTACGAGAGGATTAGTTGGTTCAGACAATTGGTACAGCAA756130.18962951058818478No Hit
GTTGGATAATGGCTGAACGCCTCTTAAGCCAGAAACCATGCTGATTAAAC751100.18836803909749064No Hit
AGAGGATTAGTTGGTTCAGACAATTGGTACAGCAATTGGTTGACAAACCA680650.17069991454094927No Hit
CGAAGCTAAGTCTGTTGGATAATGGCTGAACGCCTCTTAAGCCAGAAACC678650.1701983354194009No Hit
AAACCAGTGTTGCGAAGCTAAGTCTGTTGGATAATGGCTGAACGCCTCTT675610.16943593515464736No Hit
CCAACTTGGTACGAGAGGATTAGTTGGTTCAGACAATTGGTACAGCAATT667490.16739952392116098No Hit
GTTTTCTTTGCACTTTACCTTTATAACAAACCTTGGAATCAATTTACTTG665540.16691048427765134No Hit
GTTACTTTGAGTAAATTAGAGTGTTCAAAGCAAACAGTTAAAGCATTTAC659460.1653856837481443No Hit
GAGAGGATTAGTTGGTTCAGACAATTGGTACAGCAATTGGTTGACAAACC654160.16405649907604108No Hit
GTTCAGACAATTGGTACAGCAATTGGTTGACAAACCAGTGTTGCGAAGCT628820.15770149160602326No Hit
GGCTGAACGCCTCTTAAGCCAGAAACCATGCTGATTAAACAATACTATTT628540.15763127052900647No Hit
CAGCAATTGGTTGACAAACCAGTGTTGCGAAGCTAAGTCTGTTGGATAAT625390.15684128341256778No Hit
GTTAAAACGCTCGTAGTTGAATTTCAAAGAATCGATATTTTATTGCAACT618030.15499547224526977No Hit
CGAGTTTTTAAAAATATGCTTATATTTGTATCTTTGATGCTTATATTTTG611970.1534756875069782No Hit
GTTCTACTAATAATTTGTTTTTTACTCTATTTCTCTCTTCTTTTAAGAAT605480.15184806325755376No Hit
GTCATGTTGAGTAATTTTCCAATTAATGCCTTAGCATTCAAGCGAAGTGA557390.13978759327992318No Hit
GTTAAAACGCTCGTAGTTGAATTTCAAAGAATCGATATTTTAAGCAACGC556340.13952426424111028No Hit
GTTTAAGGCAACAACAGGTCTGTGATGTCCTTAGATGAACTAGGCTGCAC554360.1390277009107774No Hit
CAACTTGGTACGAGAGGATTAGTTGGTTCAGACAATTGGTACAGCAATTG538480.13504516268568334No Hit
GCTCAAACGGTTAATCCTTCGTTTGGTGCAAGCCTTTCGACGGAATTGTC518420.13001432409655317No Hit
GTCCTTGTTACTTTGAGTAAATTAGAGTGTTCAAAGCAAACAGATATAGC512460.12851961831433903No Hit
GAACTAGGCTGCACGCGTGCTACACTGATATATATAACGAGTTTTTAAAA511580.12829892350085775No Hit
GTTTTTTACTCTATTTCTCTCTTCTTTTAAGAATGTACTTGCTTGATTGA508390.1274989048019881No Hit
CATAATCCTATCACCTTTTTCGTGAGCTGCTGTTCGTGAGCTGTGAGCTG503470.1262650201629791No Hit
ATTTACTATTATTTTCTTATACATATAGTATTGTGACAGTAATCCAACTT502800.12609699115726042No Hit
GCAATTGGTTGACAAACCAGTGTTGCGAAGCTAAGTCTGTTGGATAATGG483580.12127681579918055No Hit
CTTCGTTTGGTGCAAGCCTTTCGACGGAATTGTCGTTGAATTATTCCATG477190.1196742705058335No Hit
ATTTACTTGGAGAAGAGGTTCGTTGAACTCAATTCAAAAAATTTCTTGAA469240.11768049349767874No Hit
ATTGGTACAGCAATTGGTTGACAAACCAGTGTTGCGAAGCTAAGTCTGTT461910.11584220601720395No Hit
GTTCAAAGCAAACAGTTAAAGCATTTACTGTGTTTGAATACTATAGCATG458180.11490676095551626No Hit
AGTTGGTTCAGACAATTGGTACAGCAATTGGTTGACAAACCAGTGTTGCG450340.11294057079904664No Hit
GAGAAATTATACGTGCTTCCTCTACGGATGCATTCATAATCCTATCACCT445830.11180950987995507No Hit
GGCTGAACGCCTCTTAAGCCAGAAACCATGCTGATTAAACAATACTATCT441780.1107938121588196No Hit
CTTGGTTCCTACAGCGATGAAGGCCGCAGCAAAATGCGATACGCAATGAA440120.11037750148793447No Hit
GCTGAACGCCTCTTAAGCCAGAAACCATGCTGATTAAACAATACTATTTC430200.10788966904505455No Hit
ATTAGTTGGTTCAGACAATTGGTACAGCAATTGGTTGACAAACCAGTGTT430050.10785205061093842No Hit
GGTTTCTTGTCAACTGTGATTGAACAAGAGTTAGCCGCTCCTAAGGGATA421640.10574291040482751No Hit
GGTTCAGACAATTGGTACAGCAATTGGTTGACAAACCAGTGTTGCGAAGC415590.1042256335621437No Hit
TTTGTTTTTTACTCTATTTCTCTCTTCTTTTAAGAATGTACTTGCTTGAT407910.10229956973539796No Hit
GTTGAATTATTCCATGAATGCCTTTAATTTGAGCAAAGTGAGCAGGAATG406680.10199109857564571No Hit
GCAAGCTTACCTTGGGAATTGTCATGTTGAGTAATTTTCCAATTAATGCC403870.10128637990987024No Hit
GTTCATTTTATTTTTTGTTTTGTAGCAATAGTAATTCGTTTTTATGAATT402050.10082994290926121No Hit

[WARN]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)