FastQCFastQC Report
Thu 2 Nov 2023
HMLTGDMXY_n02_MHC-019.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHMLTGDMXY_n02_MHC-019.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1612892
Sequences flagged as poor quality0
Sequence length101
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTCAAAAGGAAAATCCCTCGGTTTGTGCAAGCTTACCTTGGGAATTGTCA86380.5355597274957034No Hit
GTTTCTTGTCAACTGTGATTGGACAAGAGTTAGCCGCTCCTAAGGGATAG82510.5115655604963011No Hit
GGAAAATCCCTCGGTTTGTGCAAGCTTACCTTGGGAATTGTCATGTTGAG43750.27125188791313987No Hit
GTTTTCTTTGCACTTTACCTCTATAGCAAACCTTGGAATCAATTTACTTG40210.24930373515399668No Hit
GGTTTCTTGTCAACTGTGATTGGACAAGAGTTAGCCGCTCCTAAGGGATA39870.24719572048221455No Hit
GCAAGCTTACCTTGGGAATTGTCATGTTGAGTAATTTTCCAATTAATGCC34720.2152654982478678No Hit
GTTAGCCGCTCCTAAGGGATAGCTGAAAAGTGTTTATTTGGGGAATGTTT29340.18190926608849198No Hit
GTTAAAACGCTCGTAGTTGAATTTCAAAGAATCGATATTTTAAGCAACGC29190.18097925961564693No Hit
GTTGGTTCAGACAATTGGTACAGCAATTGGTTGACAAACCAGTGTTGCGA25370.1572950947738596No Hit
CCTCGGTTTGTGCAAGCTTACCTTGGGAATTGTCATGTTGAGTAATTTTC23280.1443370045855519No Hit
GTGCAAGTGTGTATGATATGTTAAACATTTGGTACTTCGCTACCTTCTAT22850.14167098603006278No Hit
GTCCTTGTTACTTTGAGTAAATTAGAGTGTTCAAAGCAAACAGATATAGC21530.13348692906902632No Hit
GGGTTTCTTGTCAACTGTGATTGGACAAGAGTTAGCCGCTCCTAAGGGAT21490.1332389273429343No Hit
TTTCTTGTCAACTGTGATTGGACAAGAGTTAGCCGCTCCTAAGGGATAGC21130.1310069118081062No Hit
GCTAAGTCTGTTGGATAATGGCTGAACGCCTCTTAAGCCAGAAACCATGC19720.1222648509633627No Hit
TGCTGATTAAACAATACTATCTCGTTCTTTTTTGTTATCCTTTTACTGCT18220.11296478623491218No Hit
GTGCAAGTGTGTATGAAATGTTAAACATTTGGTACTTCGCTACCTTCTAT18140.11246878278272816No Hit
GTCATGTTGAGTAATTTTCCAATTAATGCCTTAGCATTCAAGCGAAGTGA17790.11029876767942304No Hit
GTTTTGTAAGAAATATGCATTTCATCCCCGTCTCGAAAGGGAAACAGGTT17310.1073227469663189No Hit
CTGAAAAGTGTTTATTTGGGGAATGTTTTGTAAGAAATATGCATTTCATC16990.10533873315758278No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)