FastQCFastQC Report
Thu 2 Nov 2023
HMLTGDMXY_n02_MHC-014.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHMLTGDMXY_n02_MHC-014.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2090794
Sequences flagged as poor quality0
Sequence length101
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GTTTCTTGTCAACTGTGATTGGACAAGAGTTAGCCGCTCCTAAGGGATAG211471.0114339337113076No Hit
GGTTTCTTGTCAACTGTGATTGGACAAGAGTTAGCCGCTCCTAAGGGATA99640.4765653622499395No Hit
GTTAGCCGCTCCTAAGGGATAGCTGAAAAGTGTTTATTTGGGGAATGTTT81020.3875082863256734No Hit
GTTGGTTCAGACAATTGGTACAGCAATTGGTTGACAAACCAGTGTTGCGA74150.35464995594974924No Hit
GTTTTCTTTGCACTTTACCTCTATAGCAAACCTTGGAATCAATTTACTTG71070.3399187103081413No Hit
GCAAGCTTACCTTGGGAATTGTCATGTTGAGTAATTTTCCAATTAATGCC68010.3252831221057646No Hit
TTTCTTGTCAACTGTGATTGGACAAGAGTTAGCCGCTCCTAAGGGATAGC60630.28998552702944436No Hit
GTGCAAGTGTGTATGATATGTTAAACATTTGGTACTTCGTTACCTTCTAT58940.2819024734144062No Hit
GGGTTTCTTGTCAACTGTGATTGGACAAGAGTTAGCCGCTCCTAAGGGAT57310.2741063921170617No Hit
CTCAAAAGGAAAATCCCTCGGTTTGTGCAAGCTTACCTTGGGAATTGTCA53480.255787992504283No Hit
CTCAAAAGGAAAATCCCTTGGTTTGTGCAAGCTTACCTTGGGAATTGTCA53000.25349221396273375No Hit
GTCCTTGTTACTTTGAGTAAATTAGAGTGTTCAAAGCAAACAGATATAGC52070.24904414303848202No Hit
CAGTAATCCAACTTGGTACGAGAGGATTAGTTGGTTCAGACAATTGGTAC44700.21379437668177736No Hit
TTTTGAGTGTAATAGAGATGGTAACATATATACAAATGAACTCCTTCACA39730.1900235030328191No Hit
CAAGAGTTAGCCGCTCCTAAGGGATAGCTGAAAAGTGTTTATTTGGGGAA39540.18911475736012254No Hit
GCTAAGTCTGTTGGATAATGGCTGAACGCCTCTTAAGCCAGAAACCATGC37870.1811273611843156No Hit
GTTCGCTGAATCAATTCAGGCAAATTATTTTTTTGGGGTTTCGGCCTTGA37660.18012295807238782No Hit
CTTGTCAACTGTGATTGGACAAGAGTTAGCCGCTCCTAAGGGATAGCTGA37160.1777315220916073No Hit
GGTTGATATTCCTGTGCCGATAGTATTTTGAGTGTAATAGAGATGGTAAC36810.17605751690506097No Hit
GATTAGTTGGTTCAGACAATTGGTACAGCAATTGGTTGACAAACCAGTGT36000.17218339061619653No Hit
GTCATGTTGAGTAATTTTCCAATTAATGCCTTAGCATTCAAGCGAAGTGA34810.1664917729819389No Hit
ATTCAAGCGAAGTGAGCAGGAAAGCTCTCCCTAAGAGGGTGAAAGGCCCG33390.15970009479652228No Hit
TTCTTGTCAACTGTGATTGGACAAGAGTTAGCCGCTCCTAAGGGATAGCT32550.15568248234881102No Hit
CTTGGAATCAATTTACTTGGAGAAGAGGTTCGCTGAATCAATTCAGGCAA32180.15391281972303344No Hit
GGAAAATCCCTCGGTTTGTGCAAGCTTACCTTGGGAATTGTCATGTTGAG31540.1508517816676344No Hit
GACAATTGGTACAGCAATTGGTTGACAAACCAGTGTTGCGAAGCTAAGTC31370.15003869343416903No Hit
CTTGGAAGTCCTTGTTACTTTGAGTAAATTAGAGTGTTCAAAGCAAACAG30590.14630805330415145No Hit
TGCTGATTAAACAATACTATCTCGTTCTTTTTTGTTATCCTTTTACTGCT30510.14592542354722654No Hit
TGTGCAAGTGTGTATGATATGTTAAACATTTGGTACTTCGTTACCTTCTA29660.1418599823798997No Hit
GGAAAATCCCTTGGTTTGTGCAAGCTTACCTTGGGAATTGTCATGTTGAG28990.1386554581656538No Hit
TTTACTATTATTTTCTTATACATATAGTATTGTGACAGTAATCCAACTTG28210.13492481803563622No Hit
ATTTACTTGGAGAAGAGGTTCGCTGAATCAATTCAGGCAAATTATTTTTT27880.13334647028832108No Hit
TGTCAACTGTGATTGGACAAGAGTTAGCCGCTCCTAAGGGATAGCTGAAA27440.13124200662523425No Hit
AGGGTTTCTTGTCAACTGTGATTGGACAAGAGTTAGCCGCTCCTAAGGGA27300.1305724045506157No Hit
GTGGAGAAGGGTTTCTTGTCAACTGTGATTGGACAAGAGTTAGCCGCTCC27120.12971148759753473No Hit
GGCTGAACGCCTCTTAAGCCAGAAACCATGCTGATTAAACAATACTATCT26410.1263156485048264No Hit
TCATAATCCTATCACCTTTTTCGTGAGCTGCTGTTCGTGAGCTGTGAGCT26280.12569387514982347No Hit
GGGAAACAGGTTGATATTCCTGTGCCGATAGTATTTTGAGTGTAATAGAG26050.12459381459866442No Hit
TGTCATGTTGAGTAATTTTCCAATTAATGCCTTAGCATTCAAGCGAAGTG25300.12100666062749366No Hit
CAGCGAACTTGGATAATTATGATAAAACAATTCACTGAAGTTGTTGGGAA23750.1135932090870741No Hit
CTGAAAAGTGTTTATTTGGGGAATGTTTTGTAAGAAAATATGCATTTCAT22900.10952776791974723No Hit
CTGAAAAGTGTTTATTTGGGGAATGTTTTGTAAGAAATATGCATTTCATC22600.10809290633127892No Hit
GTTGAGTAATTTTCCAATTAATGCCTTAGCATTCAAGCGAAGTGAGCAGG22580.1079972488920477No Hit
ATTTACTATTATTTTCTTATACATATAGTATTGTGACAGTAATCCAACTT22440.10732764681742916No Hit
AGAGGATTAGTTGGTTCAGACAATTGGTACAGCAATTGGTTGACAAACCA22420.10723198937819794No Hit
TGTGACAGTAATCCAACTTGGTACGAGAGGATTAGTTGGTTCAGACAATT22370.1069928457801199No Hit
CATAATCCTATCACCTTTTTCGTGAGCTGCTGTTCGTGAGCTGTGAGCTG22300.10665804474281063No Hit
CTTGGAGAAGAGGTTCGCTGAATCAATTCAGGCAAATTATTTTTTTGGGG22300.10665804474281063No Hit
GTTTTGTAAGAAATATGCATTTCATCCCCGTCTCGAAAGGGAAACAGGTT21580.10321437693048668No Hit
CTGTGATTGGACAAGAGTTAGCCGCTCCTAAGGGATAGCTGAAAAGTGTT21520.10292740461279304No Hit
CTTTTTCGTGAGCTGCTGTTCGTGAGCTGTGAGCTGGTAATGCAAATTAT21500.10283174717356182No Hit
CAGCAATTGGTTGACAAACCAGTGTTGCGAAGCTAAGTCTGTTGGATAAT21450.10259260357548376No Hit
AATTGGTACAGCAATTGGTTGACAAACCAGTGTTGCGAAGCTAAGTCTGT21230.10154037174394034No Hit
GGATTAGTTGGTTCAGACAATTGGTACAGCAATTGGTTGACAAACCAGTG21050.10067945479085935No Hit
GACAAGAGTTAGCCGCTCCTAAGGGATAGCTGAAAAGTGTTTATTTGGGG21000.1004403111927813No Hit
ATTTTTTTGGGGTTTCGGCCTTGAATTCTTAATTTCCTGTTTTGTAGTAA20930.10010551015547205No Hit

[WARN]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)