FastQCFastQC Report
Thu 2 Nov 2023
HMLTGDMXY_n02_MHC-004.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHMLTGDMXY_n02_MHC-004.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences55910850
Sequences flagged as poor quality0
Sequence length101
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTTTCTTGTCAACTGTGATTGAACAAGAGTTAGCCGCTCCTAAGGGATAG1752440.313434691119881No Hit
GCTCAAACGGTTAATCCTTCGTTTGGTGCAAGCCTTTCGACGGAATTGTC1529600.273578384159783No Hit
GTAACAACTCACCTACCGAATGAACTAGCCCTGAAAATGGATGGCGCTAA1179180.21090360815476783No Hit
GGGAAATCGTTTTCCTTGACCTATTCTCAAACTCCCAATAGGTAAAAAGG1159350.20735689047832398No Hit
GGCAAATCCGCCGAATTTAAGCATATAATTAAGCGGAAGAAAAGAAAATA893370.15978472872438892No Hit
GTTTGACTGGGGCGGTACATCTGTTAAATATTAACGCAGATGTCCAAAGA784090.14023932742929146No Hit
CTTCGTTTGGTGCAAGCCTTTCGACGGAATTGTCGTTGAATTATTCCATG780720.1396365821660733No Hit
GTTTTCTTTGCACTTTACCTTTATAACAAACCTTGGAATCAATTTACTTG764960.13681780906568225No Hit
GGAGTCTAGCAAATGTGCAAGTGTATATGATTCTTTAAACATTTCTCTTT764960.13681780906568225No Hit
GGACATTAGAAAAGAGAAGAAAAAAAGAGGGTCGAAAATAAAATTGCAGT747540.13370213473771192No Hit
GGAATGCTTCCCCATAGAGGGTGAAAGGCCCATAGTTCGTTTTAATTTAT731750.1308779959524851No Hit
GGACTATCCTTGAAAAAAGGAGGGAACGGCTTTGTAAACTTGGTTTCTTC723170.1293434100894549No Hit
GTTGAATTATTCCATGAATGCCTTTAATTTGAGCAAAGTGAGCAGGAATG706850.12642447753879613No Hit
GAAATATATTATGCTTTATCCTTCGGGAAGGCATTCTGATATTATTTGAA678640.1213789452315606No Hit
CCCAATTCTAAAGAAGAGAGGTAGTGACAAGAAATAACAATGCAAGGCCA669540.11975135416471042No Hit
GGTTTCTTGTCAACTGTGATTGAACAAGAGTTAGCCGCTCCTAAGGGATA651510.11652657757841278No Hit
CTCAAACGGTTAATCCTTCGTTTGGTGCAAGCCTTTCGACGGAATTGTCG638260.11415673344261446No Hit
CAAAGAAGTAACACGTAATAAATTTATTTTATTTAGTGTGTATCAATCGA593100.10607958920316896No Hit
GGGGAATTAGAGTTCGATTCCGGAGAGGGAGCCTGAGAAATAGCTACCAC588630.10528010216263926No Hit
GTTGGTTCAGACAATTGGTACAGCAATTGGTTGACAAACCAGTGTTGCGA585170.10466125984491383No Hit
GTTCATTTCAATCACGTTTAGGGGCGAAAGACTAATCGAAAAGCCTATTA582820.10424094786611185No Hit
GGGAAATATATTATGCTTTATCCTTCGGGAAGGCATTCTGATATTATTTG577800.10334308993692638No Hit
CAAGGACCAGCTCAAACGGTTAATCCTTCGTTTGGTGCAAGCCTTTCGAC576130.10304440014773518No Hit
GGTAATTCTAGAGCTAATACATGCCGACGGGCGCTGACCCCCTTCGCGGG575030.10284765837042364No Hit
GACTAATCGAAAAGCCTATTAGCTGGTTATTTTCGAAAGATCTCTCAGGA575030.10284765837042364No Hit
GAAAAAAGTTATTAACTTAAGGAATTATAACAAAGAAGTAACACGTAATA570000.10194801187962622No Hit
GGTGAAGAGACATGAGAGGTGTAGAATAAGTGGGAGGCCCCCGGCGCCCC559890.10013977608997181No Hit
GGAGTGTGTAACAACTCACCTACCGAATGAACTAGCCCTGAAAATGGATG559620.10009148492644987No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)