FastQCFastQC Report
Thu 2 Nov 2023
HMLTGDMXY_n02_KHC-012.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHMLTGDMXY_n02_KHC-012.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences33903223
Sequences flagged as poor quality0
Sequence length101
%GC64

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGAGGATTCAACCCGGCGGCGGGTCCGGCCGTGTCGGCGGCCCGGCGGAT2720800.8025195716643223No Hit
GGGAGCGAGACCCGTCGCCGCGCTCTCCCCCCTCCCGGCGCCCACCCCCG1159500.3420028827347772No Hit
CTTGACTCTAGTCTGGCACGGTGAAGAGACATGAGAGGTGTAGAATAAGT1067130.3147576854271348No Hit
ATTCAACCCGGCGGCGGGTCCGGCCGTGTCGGCGGCCCGGCGGATCTTTC1017950.30025168993520174No Hit
CGGAACGATACGGCAGCGCCGCGGAGCCTCGGTTGGCCTCGGATAGCCGG991120.29233798804320166No Hit
CCGGCGGCGGGTCCGGCCGTGTCGGCGGCCCGGCGGATCTTTCCCGCCCC978000.2884681494735766No Hit
GTGGGAGGCCCCCGGCGCCCCCCCGGTGTCCCCGCGAGGGGCCCGGGGCG863180.2546011628451962No Hit
GTTACAGCCCCCCCGGCAGCAGCACTCGCCGAATCCCGGGGCCGAGGGAG794570.2343641488008382No Hit
GCGAGACCCGTCGCCGCGCTCTCCCCCCTCCCGGCGCCCACCCCCGCGGG730990.2156107695129752No Hit
GGTGAAGAGACATGAGAGGTGTAGAATAAGTGGGAGGCCCCCGGCGCCCC730570.21548688748559394No Hit
GTCCGCCCGGAGGATTCAACCCGGCGGCGGGTCCGGCCGTGTCGGCGGCC681030.20087470739876262No Hit
GAGGATTCAACCCGGCGGCGGGTCCGGCCGTGTCGGCGGCCCGGCGGATC677790.19991904604467842No Hit
CCGGGGCCGAGGGAGCGAGACCCGTCGCCGCGCTCTCCCCCCTCCCGGCG654280.19298460208340665No Hit
GGTCCGCGCAGTCCGCCCGGAGGATTCAACCCGGCGGCGGGTCCGGCCGT619600.18275548610820866No Hit
GAGACATGAGAGGTGTAGAATAAGTGGGAGGCCCCCGGCGCCCCCCCGGT600910.17724273588974124No Hit
AGAAGACCCTGTTGAGCTTGACTCTAGTCTGGCACGGTGAAGAGACATGA530640.1565160928800191No Hit
GAACGATACGGCAGCGCCGCGGAGCCTCGGTTGGCCTCGGATAGCCGGTC492470.1452575762487242No Hit
CTCTAGTCTGGCACGGTGAAGAGACATGAGAGGTGTAGAATAAGTGGGAG483090.14249087763720872No Hit
GTCCGCGCAGTCCGCCCGGAGGATTCAACCCGGCGGCGGGTCCGGCCGTG458240.13516119101714902No Hit
GAGCGAGACCCGTCGCCGCGCTCTCCCCCCTCCCGGCGCCCACCCCCGCG446420.13167479681798985No Hit
GCGCAGTCCGCCCGGAGGATTCAACCCGGCGGCGGGTCCGGCCGTGTCGG422570.12464006740598084No Hit
GTAGAATAAGTGGGAGGCCCCCGGCGCCCCCCCGGTGTCCCCGCGAGGGG394830.11645795445465465No Hit
CGATACGGCAGCGCCGCGGAGCCTCGGTTGGCCTCGGATAGCCGGTCCCC378300.11158231180557672No Hit
GCTTGACTCTAGTCTGGCACGGTGAAGAGACATGAGAGGTGTAGAATAAG366210.10801627916024385No Hit
CCGGAGGATTCAACCCGGCGGCGGGTCCGGCCGTGTCGGCGGCCCGGCGG357050.1053144711344995No Hit
TTCAACCCGGCGGCGGGTCCGGCCGTGTCGGCGGCCCGGCGGATCTTTCC350560.10340019885425053No Hit
GGGGGGGCGAGCCCCGAGGGGCTCTCGCTTCTGGCGCCAAGCGCCCGGCC344560.10163045560594637No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)