FastQCFastQC Report
Thu 2 Nov 2023
HMLTGDMXY_n02_KHC-005.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHMLTGDMXY_n02_KHC-005.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2265592
Sequences flagged as poor quality0
Sequence length101
%GC42

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTTTCTTGTCAACTGTGATTGGACAAGAGTTAGCCGCTCCTAAGGGATAG193220.852845525584483No Hit
CTCAAAAGGAAAATCCCTCGGTTTGTGCAAGCTTACCTTGGGAATTGTCA184660.815062906295573No Hit
GGAAAATCCCTCGGTTTGTGCAAGCTTACCTTGGGAATTGTCATGTTGAG129010.569431742343723No Hit
GTTTTCTTTGCACTTTACCTCTATAGCAAACCTTGGAATCAATTTACTTG98410.4343677061006571No Hit
GGTTTCTTGTCAACTGTGATTGGACAAGAGTTAGCCGCTCCTAAGGGATA74130.3271992485849173No Hit
GCAAGCTTACCTTGGGAATTGTCATGTTGAGTAATTTTCCAATTAATGCC66500.29352151667202214No Hit
GTCATGTTGAGTAATTTTCCAATTAATGCCTTAGCATTCAAGCGAAGTGA65190.287739363486453No Hit
GAGAAATTATACGTGCTTCCTCTACGGATGCATTCATAATCCTATCACCT60850.26858322239838417No Hit
GTTAGCCGCTCCTAAGGGATAGCTGAAAAGTGTTTATTTGGGGAATGTTT57780.25503268020014197No Hit
ATTTACTTGGAGAAGAGGTTCGCTGAATCAATTCAGGCAAATTATTTTTT53970.23821588353066217No Hit
GGGTTTCTTGTCAACTGTGATTGGACAAGAGTTAGCCGCTCCTAAGGGAT53960.23817174495672655No Hit
GTCCTTGTTACTTTGAGTAAATTAGAGTGTTCAAAGCAAACAGATATAGC49350.2178238623723954No Hit
CAAGAGTTAGCCGCTCCTAAGGGATAGCTGAAAAGTGTTTATTTGGGGAA47900.21142376915172725No Hit
CTGAAAAGTGTTTATTTGGGGAATGTTTTGTAAGAAATATGCATTTCATC47170.20820165325442533No Hit
AATCAATTTACTTGGAGAAGAGGTTCGCTGAATCAATTCAGGCAAATTAT44020.19429800246469797No Hit
CCTCGGTTTGTGCAAGCTTACCTTGGGAATTGTCATGTTGAGTAATTTTC43590.19240004378546535No Hit
TTTCTTGTCAACTGTGATTGGACAAGAGTTAGCCGCTCCTAAGGGATAGC43310.19116416371526737No Hit
GTTGGTTCAGACAATTGGTACAGCAATTGGTTGACAAACCAGTGTTGCGA42330.18683858346957438No Hit
CGAAAGGGAAACAGGTTGATATTCCTGTGCCGATAGTATTTTGAGTGTAA41840.18467579334672793No Hit
CATAATCCTATCACCTTTTTCGTGAGCTGCTGTTCGTGAGCTGTGAGCTG41810.18454337762492098No Hit
GTGGAGAAGGGTTTCTTGTCAACTGTGATTGGACAAGAGTTAGCCGCTCC41770.18436682332917842No Hit
GGGAAACAGGTTGATATTCCTGTGCCGATAGTATTTTGAGTGTAATAGAG41230.18198334033665373No Hit
AGGGTTTCTTGTCAACTGTGATTGGACAAGAGTTAGCCGCTCCTAAGGGA39600.17478875278514402No Hit
GGTTGATATTCCTGTGCCGATAGTATTTTGAGTGTAATAGAGATGGTAAC38930.17183146833145596No Hit
GTTTTGTAAGAAATATGCATTTCATCCCCGTCTCGAAAGGGAAACAGGTT37520.1656079294065304No Hit
CAAAAGGAAAATCCCTCGGTTTGTGCAAGCTTACCTTGGGAATTGTCATG37350.16485757364962447No Hit
GTGCAAGTGTGTATGATATGTTAAACATTTGGTACTTCGTTACCTTCTAT36950.16309203069219877No Hit
GTGCAAGTGTGTATGATATGTTAAACATTTGGTACTTCGCTACCTTCTAT36750.16220925921348592No Hit
CTTGGAATCAATTTACTTGGAGAAGAGGTTCGCTGAATCAATTCAGGCAA36020.15898714331618402No Hit
AGCAAACCTTGGAATCAATTTACTTGGAGAAGAGGTTCGCTGAATCAATT35970.1587664504465058No Hit
GCTAAGTCTGTTGGATAATGGCTGAACGCCTCTTAAGCCAGAAACCATGC35030.15461742449655544No Hit
GGAACATCAGCGAACTTGGATAATTATGATAAAACAATTCACTGAAGTTG34650.152940158687001No Hit
GTAAAAGAGAAATTATACGTGCTTCCTCTACGGATGCATTCATAATCCTA34530.1524104957997733No Hit
GCTGAAAAGTGTTTATTTGGGGAATGTTTTGTAAGAAATATGCATTTCAT34350.15161600146893173No Hit
ATTTACTATTATTTTCTTATACATATAGTATTGTGACAGTAATCCAACTT34320.1514835857471248No Hit
ATTCAAGCGAAGTGAGCAGGAAAGCTCTCCCTAAGAGGGTGAAAGGCCCG33370.1472904212232388No Hit
CTTGTCAACTGTGATTGGACAAGAGTTAGCCGCTCCTAAGGGATAGCTGA32180.14203793092489733No Hit
GTTGAGTAATTTTCCAATTAATGCCTTAGCATTCAAGCGAAGTGAGCAGG32040.14141999088979834No Hit
GTTAAACATTTGGTACTTCGCTACCTTCTATACGCACAATTAATGTAATA31560.13930133934088748No Hit
TCATAATCCTATCACCTTTTTCGTGAGCTGCTGTTCGTGAGCTGTGAGCT30630.13519645196487276No Hit
CAGCGAACTTGGATAATTATGATAAAACAATTCACTGAAGTTGTTGGGAA29800.13153295032821444No Hit
GTTGATATTCCTGTGCCGATAGTATTTTGAGTGTAATAGAGATGGTAACA28710.1267218457692294No Hit
TTCTTGTCAACTGTGATTGGACAAGAGTTAGCCGCTCCTAAGGGATAGCT28470.12566251999477399No Hit
CTTGGAGAAGAGGTTCGCTGAATCAATTCAGGCAAATTATTTTTTTGGGG28070.1238969770373483No Hit
TCAAAAGGAAAATCCCTCGGTTTGTGCAAGCTTACCTTGGGAATTGTCAT27150.1198362282352692No Hit
GGCTGAACGCCTCTTAAGCCAGAAACCATGCTGATTAAACAATACTATCT26710.11789413098210093No Hit
GAACTATCCTTGAAACAAGGAGGGAACAGCAATTAAAGGGGGATGATTTA26600.11740860666880887No Hit
CAGTAATCCAACTTGGTACGAGAGGATTAGTTGGTTCAGACAATTGGTAC26370.11639341946828909No Hit
ATTTTTTTGGGGTTTCGGCCTTGAATTCTTAATTTCCTGTTTTGTAGTAA26140.1153782322677693No Hit
CTTTACTCTAGTCTGGCTTTGTGAAACGACTTAAGAGGTGTAGCATAAGT26140.1153782322677693No Hit
GTTTATTTGGGGAATGTTTTGTAAGAAATATGCATTTCATCCCCGTCTCG26130.11533409369383367No Hit
AAACGACTTAAGAGGTGTAGCATAAGTGGGAGTAGAGACTGAAATATGTT26000.1147602922326703No Hit
GATTAGTTGGTTCAGACAATTGGTACAGCAATTGGTTGACAAACCAGTGT25890.11427476791937825No Hit
TGGGAATTGTCATGTTGAGTAATTTTCCAATTAATGCCTTAGCATTCAAG25780.11378924360608618No Hit
GGAGAAGGGTTTCTTGTCAACTGTGATTGGACAAGAGTTAGCCGCTCCTA25780.11378924360608618No Hit
GGAGTCTAGCAAATGTGCAAGTGTGTATGATATGTTAAACATTTGGTACT25060.11061126628271992No Hit
GTTAAACATTTGGTACTTCGTTACCTTCTATACGCACAATTAATGTAATA24870.10977263337794271No Hit
GTTAAAACGCTCGTAGTTGAATTTCAAAGAATCGATATTTTAAGCAACGC24870.10977263337794271No Hit
GACAAGAGTTAGCCGCTCCTAAGGGATAGCTGAAAAGTGTTTATTTGGGG24720.10911055476890809No Hit
GTAAGAAATATGCATTTCATCCCCGTCTCGAAAGGGAAACAGGTTGATAT24670.10888986189922986No Hit
CAAGGACCAGCTCAAAAGGAAAATCCCTCGGTTTGTGCAAGCTTACCTTG24480.10805122899445266No Hit
AGGGAAACAGGTTGATATTCCTGTGCCGATAGTATTTTGAGTGTAATAGA24200.10681534892425468No Hit
GAAAAGTGTTTATTTGGGGAATGTTTTGTAAGAAATATGCATTTCATCCC24170.10668293320244773No Hit
GTTCGCTGAATCAATTCAGGCAAATTATTTTTTTGGGGTTTCGGCCTTGA24140.10655051748064083No Hit
CTGAAATATGTTTTTACGACAGTGAAATACCACTACTTTTAAAGTTGTTT23560.10399048019237357No Hit
CGGAAAAGCTGTAGCTAATACTTGCTTTAGCACTCTTGATTCATTTCTTG23500.1037256487487597No Hit
AGTAATTCGTTTTTATGAATTATCCGAAGCCGTAAGAACTATCCTTGAAA23450.1035049558790815No Hit
AGAAATTATACGTGCTTCCTCTACGGATGCATTCATAATCCTATCACCTT23400.10328426300940328No Hit
GTTTTCTTTGCACTTTACCTCTATAGCAAGCCTTGGAATCAATTTACTTG23160.10222493723494785No Hit
CTTGGAAGTCCTTGTTACTTTGAGTAAATTAGAGTGTTCAAAGCAAACAG23130.10209252151314094No Hit
AACGACTTAAGAGGTGTAGCATAAGTGGGAGTAGAGACTGAAATATGTTT22930.1012097500344281No Hit
GTGCAAGTGTGTATGAAATGTTAAACATTTGGTACTTCGCTACCTTCTAT22750.10041525570358653No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)