FastQCFastQC Report
Thu 2 Nov 2023
HMLTGDMXY_n01_MTH-019.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHMLTGDMXY_n01_MTH-019.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences39244068
Sequences flagged as poor quality0
Sequence length101
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CCCGAGTGTAAAGCCTATGTAAAGGAGTTCATTTATATATATGTTACCAT1843620.46978310199645973No Hit
CTCCAATCTCAAAGAACACGACTCGGCATGAAAAATTCCAATGAATAAAT1510650.38493715789097094No Hit
CCCCAAGCTACTCCTATTAATCGTAACTAAGCCAAAATAAGAAAAAAGAA1354830.3452317940127919No Hit
CTCCCGTCCACTCTCGACTGCCGGCGACGGCCGGGTATGGGCCCGACGCT1208090.3078401556128177No Hit
GTCCACTCTCGACTGCCGGCGACGGCCGGGTATGGGCCCGACGCTCCAGC1048060.26706201813736535No Hit
CACCGGGTCAGTGAAAAAACGATCAGAGTAGTGGTATTTCACCGGCGGCC1011120.2576491305641403No Hit
CTCCTACACATATTTAGCTTTATGTGAAATGTATCACACGTATTAGTGCT970930.24740809235168992No Hit
CCACTCTCGACTGCCGGCGACGGCCGGGTATGGGCCCGACGCTCCAGCGC940630.23968718023829744No Hit
CCTACACATATTTAGCTTTATGTGAAATGTATCACACGTATTAGTGCTCC919210.23422903048684962No Hit
CCCCGTTACCCGTCATAGCCATGTTAGGCCAATACCCTAACATCAAAAGC889500.22665845956642414No Hit
CTACAGTTTGTGCAATTCTTTTTTGTTGTTAAAGAACTATTGCCTAGCTC889320.22661259276179013No Hit
CCCCGCCTCACCGGGTCAGTGAAAAAACGATCAGAGTAGTGGTATTTCAC861060.21941150443424978No Hit
GCCCTTTTTACCTATTGGGAGTTTGAGAATAGGTCAAGGAAAACGATTTC842680.21472799404995427No Hit
GTCCGTTTCAGTTCTGAGTTAACATTATAGGTATCAAATTAAAGTAATAA830460.21161414764646722No Hit
GGCACCTTATCCAAGCGTTAGGAGCATCCCTCATCGCCAGTTCTGCTTAC820420.2090557992102144No Hit
CGCTCATACAAGTATTAAGAGTATAGCAAAGCAAAATGGAATCAGAAGAA721370.18381631588244113No Hit
GCTCATACAAGTATTAAGAGTATAGCAAAGCAAAATGGAATCAGAAGAAA716540.18258555662476172No Hit
CCCGTTACCCGTCATAGCCATGTTAGGCCAATACCCTAACATCAAAAGCT690370.17591703286213856No Hit
CCGAGTGTAAAGCCTATGTAAAGGAGTTCATTTATATATATGTTACCATC665570.1695976064458965No Hit
CCGGAATCGAACTCTAATTCCCCGTTACCCGTCATAGCCATGTTAGGCCA649230.16543391984745312No Hit
CTCGGGTCCTACCAAATATGCTCGTACTCAATAAAGCATAATTGCTTACC646350.1647000509733089No Hit
CGCCCTTTTTACCTATTGGGAGTTTGAGAATAGGTCAAGGAAAACGATTT613220.1562580107648371No Hit
GGCAGACGTTCGAATGGGTCGTCGCCGCCACGGGGGGCGTGCGATCGGCC598650.1525453477452949No Hit
CTCGACTGCCGGCGACGGCCGGGTATGGGCCCGACGCTCCAGCGCCATCC594020.15136555160387552No Hit
CTTAACTTTCGTTCTTGATTAATGGAAGTATTTTAGACAAATGCTTTCGC585620.14922510072095482No Hit
CACATATTTAGCTTTATGTGAAATGTATCACACGTATTAGTGCTCCAATC558160.14222786485845454No Hit
GTCGTCTACAGTTTGTGCAATTCTTTTTTGTTGTTAAAGAACTATTGCCT557760.1421259386259345No Hit
GTCTCCTACACATATTTAGCTTTATGTGAAATGTATCACACGTATTAGTG553390.14101239453565315No Hit
GCCCCATTGGCTCCTCAGCCAAGCACATACACCAAATGTCTGAACCTGCG544820.13882862500391144No Hit
CTACAGTTTGTGCAATTCTTTTTTGTTGTTAAAGAACTATTTGCCTAGCT542930.13834702355525427No Hit
CTTAAACTTCCTTGTGTTAGACACACAATGTTCCTCTAAGAAGCTTTTCA528560.13468532365197206No Hit
CCTGTGGTAACTTTTCAGACACCTCTATATGCACAGTAGTAAGTAATTTA528320.13462416791246004No Hit
CCCACTTGGAGTTATTTTATTTAATATGAGTGAAATACAAATTGAGTTAT527100.13431329290327393No Hit
GTACGCTCATACAAGTATTAAGAGTATAGCAAAGCAAAATGGAATCAGAA503590.128322578586909No Hit
CTTTTAGTCTACAAGAGATTTCTGTTCTCTTTGAGCTTGTCTTTGGACAT490250.12492333873236589No Hit
CTTCCTTGGATGTGGTAGCCGTTTCTCAGGCTCCCTCTCCGGAATCGAAC487010.12409773624895361No Hit
CCATGCTATAGTATTCAAACACAGTAAATGCTTTAACTGTTTGCTTTGAA482740.12300967371680223No Hit
CCTCGTTCAAGATTAATAATTGCAATAATCTATCCCCATCACGATATATA465400.11859117153705881No Hit
CCCGGGGCTCCCGCCGGCTTCTCCGGGATCGGTCGCGTTACCGCACTGGA463290.11805351066051563No Hit
GTTGCCTTAAACTTCCTTGTGTTAGACACACAATGTTCCTCTAAGAAGCT451790.11512313147556466No Hit
GTCAGTATCAACTCGATCCTCCATCAGACTTTCGTCTGACTTCAATCTCA451640.11508490913836963No Hit
CTCTCGGGTCCTACCAAATATGCTCGTACTCAATAAAGCATAATTGCTTA444810.11334451871809008No Hit
CTCCTTTTTTCAAGGATAGTCCTTACCACTTCGGATAATTCATAAAAACG438750.11180033629541158No Hit
CCCCGTTCAAGAAATTTTTTGAATTGAGTTCAACGAACCTCTTCTCCAAG436570.1112448383281774No Hit
GGCGCCAGAAGCGAGAGCCCCTCGGGGCTCGCCCCCCCGCCTCACCGGGT431700.110003886447246No Hit
GCCGTATCGTTCCGCCTGGGCGGGATTCTGACTTAGAGGCGTTCAGTCAT425730.10848263742688449No Hit
GTTACTTCTTTGTTATAATTCCTTAAGTTAATAACTTTTTTCTTATAAGG424380.10813863639212938No Hit
CTCCACTTCAGTCTTCAAAGTTCTCATTTGAATAGTTGCTACTTCCACCA413100.10526431663506444No Hit
CCCGCCTCACCGGGTCAGTGAAAAAACGATCAGAGTAGTGGTATTTCACC402410.10254033807096655No Hit
GTCCTACCAAATATGCTCGTACTCAATAAAGCATAATTGCTTACCGGTAT401840.1023950931896255No Hit
CCAGAACCCAAAGACTTTGATTTCTCATAAGGTACTGAAGGAAGCAATCT398000.10141660135743318No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)