FastQCFastQC Report
Thu 2 Nov 2023
HMLTGDMXY_n01_MHC-090.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHMLTGDMXY_n01_MHC-090.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences26473661
Sequences flagged as poor quality0
Sequence length101
%GC39

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CCCCGTTACCCGTCATAGCCATGTTAGGCCAATACCCTAACATCAAAAGC3329941.2578313214783554No Hit
CTACAGTTTGTGCAATTCTTTTTTGTTGTTAAAGAACTATTGCCTAGCTC3186281.2035660651543434No Hit
CTTTAATTGCTGTTCCCTCCTTGTTTCAAGGATAGTTCTTACGGCTTCGG3018661.140250303877503No Hit
CCTGAATTGATTCAGCGAACCTCTTCTCCAAGTAAATTGATTCCAAGGTT2868971.0837073119581007No Hit
CCCCAAGCTACTCCTATTAATCGTAACTAAGCCAAAATAAGAAAAAAGAA2675951.010797108869831No Hit
CTCCAATCTCAAAGAACACGACTCTAGAAGTAAAATTCCAAAAAATAGAA2170190.8197543966435168No Hit
CTCCTACACATATTTAGCTTTATGTGAAATGTATCACACGAATTAGTGCT2107190.795957159079736No Hit
CCCGTTACCCGTCATAGCCATGTTAGGCCAATACCCTAACATCAAAAGCT2028420.7662030574464181No Hit
CCTACACATATTTAGCTTTATGTGAAATGTATCACACGAATTAGTGCTCC1942150.7336159513412217No Hit
GTCGTCTACAGTTTGTGCAATTCTTTTTTGTTGTTAAAGAACTATTGCCT1356160.5122676459443973No Hit
CCCGAGGCAAAAAAGTCGTCTACAGTTTGTGCAATTCTTTTTTGTTGTTA1317470.49765312020879926No Hit
CTCGGGTCCTACCAAATATGCTCTTACTCAATATAGCGTAATTGCTTACC1290310.48739386668130263No Hit
CTCTCGGGTCCTACCAAATATGCTCTTACTCAATATAGCGTAATTGCTTA1286100.4858036068377547No Hit
CTGAATTGATTCAGCGAACCTCTTCTCCAAGTAAATTGATTCCAAGGTTT1233540.465949911498829No Hit
GTCTCCTACACATATTTAGCTTTATGTGAAATGTATCACACGAATTAGTG1219730.46073340593127643No Hit
CCCCCTTTAATTGCTGTTCCCTCCTTGTTTCAAGGATAGTTCTTACGGCT1201990.4540324060204593No Hit
CCCCTTTAATTGCTGTTCCCTCCTTGTTTCAAGGATAGTTCTTACGGCTT1144340.4322560449799519No Hit
CCCCGAGGCAAAAAAGTCGTCTACAGTTTGTGCAATTCTTTTTTGTTGTT985060.37209058467584066No Hit
CCTTTAATTGCTGTTCCCTCCTTGTTTCAAGGATAGTTCTTACGGCTTCG953240.3600710910364834No Hit
CACATATTTAGCTTTATGTGAAATGTATCACACGAATTAGTGCTCCAATC936180.3536269501977834No Hit
CCCTTTAATTGCTGTTCCCTCCTTGTTTCAAGGATAGTTCTTACGGCTTC922200.34834622986220154No Hit
CCCGAGTGTAAGACCTATGTGAAGGAGTTCATTTGTATATATGTTACCAT918440.3469259502869664No Hit
CTCTATTACACTCAAAATACTATCGGCACAGGAATATCAACCTGTTTCCC883580.33375814550167426No Hit
CCCGTCATAGCCATGTTAGGCCAATACCCTAACATCAAAAGCTGATAGGT825470.3118080268535583No Hit
CCCCCAAGCTACTCCTATTAATCGTAACTAAGCCAAAATAAGAAAAAAGA780320.29475333993284875No Hit
GTCCTACCAAATATGCTCTTACTCAATATAGCGTAATTGCTTACCGGTAT680320.25697994697446647No Hit
CCCCTTTTAGTCTACAAGAGATTTCTGTTCTCTTTGAGCTTGTCTTTGGA668760.25261334274847747No Hit
CTCCTTGTTTCAAGGATAGTTCTTACGGCTTCGGATAATTCATAAAAACG662140.2501127441346325No Hit
CCATGCTGTAGTATTCAAACGCGCAATGCTATATCTGTTTGCTTTGAACA661680.24993898652702398No Hit
GTACGCTCATACAAGTATTAAGAGTATACAAAAAAAGGTTAATGAAATAA661200.24975767424082373No Hit
GAATTGATTCAGCGAACCTCTTCTCCAAGTAAATTGATTCCAAGGTTTGC652630.24652049446429036No Hit
CTTTTAGTCTACAAGAGATTTCTGTTCTCTTTGAGCTTGTCTTTGGACAT643030.24289424874028567No Hit
CCGTTACCCGTCATAGCCATGTTAGGCCAATACCCTAACATCAAAAGCTG639730.24164772677265905No Hit
AATTGATTCAGCGAACCTCTTCTCCAAGTAAATTGATTCCAAGGTTTGCT621560.234784301272121No Hit
CTCCTATTAATCGTAACTAAGCCAAAATAAGAAAAAAGAACAAAATTCTG612620.2314073599416416No Hit
TACAGTTTGTGCAATTCTTTTTTGTTGTTAAAGAACTATTGCCTAGCTCA609910.23038370099246946No Hit
CGCTCATACAAGTATTAAGAGTATACAAAAAAAGGTTAATGAAATAATTA596400.225280515603792No Hit
CTTAACTTTCGTTCTTGATTAATGGAAGTATTTTAGGCAAATGCTTTCGC589270.22258727268585934No Hit
GTCACTTCTTTGTTATAATTCCTTAAGTTAATAACTTTTTTCTTAAAAGG589050.2225041712213509No Hit
GCACCATGTAGGAACAACTCAAACAGCAAAGAAATAAATCAATGCAATTT581680.21972027216031814No Hit
CCGGAATCGAACTCTAATTCCCCGTTACCCGTCATAGCCATGTTAGGCCA579140.21876082797917523No Hit
GTCTACAGTTTGTGCAATTCTTTTTTGTTGTTAAAGAACTATTGCCTAGC535520.20228407397072887No Hit
GCTTCGGATAATTCATAAAAACGAATTACTATTACTACAAAACAGGAAAT532350.20108665741394816No Hit
CTACACATATTTAGCTTTATGTGAAATGTATCACACGAATTAGTGCTCCA527410.19922065180180407No Hit
CTTCGGATAATTCATAAAAACGAATTACTATTACTACAAAACAGGAAATT525640.1985520627464407No Hit
CCCATCACGATATGTACTGATAAAGATTACCTACACTTTTCAGTGGAGGA524380.19807611799516509No Hit
CATCTCTATTACACTCAAAATACTATCGGCACAGGAATATCAACCTGTTT487190.1840281931539427No Hit
CTCCAATCTCAAAGAACACGACTCTAGAAGTAAAATTCCAAAAAATATAG481670.18194310186263998No Hit
GTCACGCTTAGTTCAAAGCCTCTCGGGTCCTACCAAATATGCTCTTACTC481390.18183733636235655No Hit
CCCACTTGGAGTTATATTATTTGACAAACGAGCAAAATACAGCGAAGATA476360.1799373346965499No Hit
CTCCGGAATCGAACTCTAATTCCCCGTTACCCGTCATAGCCATGTTAGGC461910.17447907941406368No Hit
CCCCTTTTGAGAAGAGTTATAAAACCAGTTGTCCCCTCGTTAGAGTGGGG458300.17311545992826605No Hit
GCTCATACAAGTATTAAGAGTATACAAAAAAAGGTTAATGAAATAATTAA445450.16826157893311394No Hit
CCTCCTTGTTTCAAGGATAGTTCTTACGGCTTCGGATAATTCATAAAAAC445450.16826157893311394No Hit
CGGATAATTCATAAAAACGAATTACTATTACTACAAAACAGGAAATTAAG445230.16817847746860548No Hit
CTACCAAATATGCTCTTACTCAATATAGCGTAATTGCTTACCGGTATTGT441310.1666977604646369No Hit
GTTAAGAAAGCACCATGTAGGAACAACTCAAACAGCAAAGAAATAAATCA437100.16510750062108903No Hit
GTCCGTTTCAGTTCTGAGTTAACATTATTAGTATCAAGAGGGACGACGCC431680.1630601827227447No Hit
GTCGCTTCTTTGTTATAATTCCTTAAGTTAATAACTTTTTTCTTAAAAGG399300.15082915808282052No Hit
GGATAATTCATAAAAACGAATTACTATTACTACAAAACAGGAAATTAAGA383290.1447816378701835No Hit
GTAGTATTCAAACGCGCAATGCTATATCTGTTTGCTTTGAACACTCTAAT365790.1381712941024666No Hit
CCCTTTTAGTCTACAAGAGATTTCTGTTCTCTTTGAGCTTGTCTTTGGAC364790.13779356017288277No Hit
GGTCCTACCAAATATGCTCTTACTCAATATAGCGTAATTGCTTACCGGTA359590.1358293437390469No Hit
CCCGAGTGTAAGACCTATGTGAAGGAGTTCATTTGTATGTATGTTACCAT357860.13517586404086687No Hit
GTTGCCTTAAACTTCCTTGTGTTAGACACACATCGTTCCTCTAAGAAGCT356730.13474902470043715No Hit
AAAAAAGTCGTCTACAGTTTGTGCAATTCTTTTTTGTTGTTAAAGAACTA354980.13408799032366547No Hit
CTTAAACTTCCTTGTGTTAGACACACATCGTTCCTCTAAGAAGCTTTACA353490.13352516676858558No Hit
GTTCCCTCCTTGTTTCAAGGATAGTTCTTACGGCTTCGGATAATTCATAA352690.13322297962491852No Hit
CTCCAATCTCAAAGAACACGACTCTAGAAGTAAAATTCCAAGAAATAGAA352040.13297745257068902No Hit
CTTTTTTGTTGTTAAAGAACTATTGCCTAGCTCACAGCTCAAATAGTCCA351570.13279991762378465No Hit
CCCATCAATAGATAAGTAAAACAACTTTAAAAGTAGTGGTATTTCACTGT350710.13247506644434254No Hit
CTCGTCACGCTTAGTTCAAAGCCTCTCGGGTCCTACCAAATATGCTCTTA343730.12983848361584746No Hit
CTCCCTTAACTTTCGTTCTTGATTAATGGAAGTATTTTAGGCAAATGCTT341220.12889037145259208No Hit
GTCAGTATCAATTCGATCTTCCATCAGACTTTCGTCTGACTTCAATCTCG338030.12768540021721966No Hit
CCCAAGCTACTCCTATTAATCGTAACTAAGCCAAAATAAGAAAAAAGAAC336590.12714146335861895No Hit
CTTTTGAGAAGAGTTATAAAACCAGTTGTCCCCTCGTTAGAGTGGGGACT318330.12024404180441836No Hit
CGCTCATACAATTATTAAGAGTATACAAAAAAAGGTTAATGAAATAATTA312490.11803807565564885No Hit
CCCCAGTCAAACTCCCCACCTGGCTATGTCTCCATAGAGTCGGGAGGAAA310500.11728638513577702No Hit
CCCCAGTCAAACTCCCCACCTGGCTATGTCTCCATAGAGTCGGGAGAAAA309790.11701819404577252No Hit
GTCAGAAACTCGATTGATACACACTAAAAATGGACGGATCCATTACGTGT303180.11452137277122344No Hit
GTACGCTCATACAAGTATTAAGAGTATACAAAAAAAAGTTAATGAAATAA301200.11377345959064748No Hit
CCTCGTTCAAGATTAATAATTGCAATAATCTATCCCCATCACGATATGTA298800.11286689815964629No Hit
CTCGATTGATACACACTAAAAATGGACGGATCCATTACGTGTCACTTCTT297770.11247783221217496No Hit
CCTTGTTTCAAGGATAGTTCTTACGGCTTCGGATAATTCATAAAAACGAA290400.10969393315114219No Hit
GTTCCTTTTAATAAAGAAGAAAAATAAATCCGAAGAATCATTTTCCCGCT285950.10801301716449416No Hit
GGCACCTTATCCAAGCGTTAGGAGCATCCCTCATCGCCAGTTCTGCTTAC284430.10743886159152677No Hit
CGTCTACAGTTTGTGCAATTCTTTTTTGTTGTTAAAGAACTATTGCCTAG279340.10551619588994511No Hit
CACGAAAAAGGTGATAGGATTATGAATGCATCCGTAGAGGAAGCACGTAT278420.105168680674728No Hit
ACACATATTTAGCTTTATGTGAAATGTATCACACGAATTAGTGCTCCAAT278370.10514979397824879No Hit
CGCTCATACAAGTATTAAGAGTATACAAAAAAAAGTTAATGAAATAATTA277460.10480605610232752No Hit
GCCCCCAAGCTACTCCTATTAATCGTAACTAAGCCAAAATAAGAAAAAAG266770.10076808039507644No Hit
CATCACGATATGTACTGATAAAGATTACCTACACTTTTCAGTGGAGGAAA265690.10036012775112592No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)