FastQCFastQC Report
Thu 2 Nov 2023
HMLTGDMXY_n01_MHC-088.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHMLTGDMXY_n01_MHC-088.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2652026
Sequences flagged as poor quality0
Sequence length101
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CCTACACATATTTAGCTTTATGTGAAATGTATCACACGAATTAGTGCTCC159390.6010122072709695No Hit
CCCCGCCTCACCGGGTCAGTGAAAAAACGATCAGAGTAGTGGTATTTCAC156020.5883049412034422No Hit
CCCCAAGCTACTCCTATTAATCGTAACTAAGCCAAAATAAGAAAAAAGAA134510.5071971390929049No Hit
CTCCTACACATATTTAGCTTTATGTGAAATGTATCACACGAATTAGTGCT111930.4220546857383751No Hit
CCCCGTTACCCGTCATAGCCATGTTAGGCCAATACCCTAACATCAAAAGC110460.41651175365550713No Hit
CTCCAATTGAGTTTTGGGAAGGTTTTAAATTCCCATCATTCCAATTACAA108370.4086309862723819No Hit
CTCCCGTCCACTCTCGACTGCCGGCGACGGCCGGGTATGGGCCCGACGCT103560.3904939091849024No Hit
CCCGTTACCCGTCATAGCCATGTTAGGCCAATACCCTAACATCAAAAGCT101810.3838951805148215No Hit
CTCCAATCTCAAAGAACACGACTCTAGAAGTAAAATTCCAAAAAATAGAA99310.37446842527184876No Hit
CTACAGTTTGTGCAATTCTTTTTTGTTGTTAAAGAACTATTGCCTAGCTC97920.36922714935675593No Hit
CTTAACTTTCGTTCTTGATTAATGGAAGTATTTTAGGCAAATGCTTTCGC91600.34539631210252086No Hit
CTCGGGTCCTACCAAATATGCTCTTACTCAATATAGCGTAATTGCTTACC89010.3356301936708011No Hit
GTCCACTCTCGACTGCCGGCGACGGCCGGGTATGGGCCCGACGCTCCAGC86770.32718382097309756No Hit
CTTTAATTGCTGTTCCCTCCTTGTTTCAAGGATAGTTCTTACGGCTTCGG83540.3150044531991768No Hit
CCCATCAATAGATAAGTAAAACAACTTTAAAAGTAGTGGTATTTCACTGT83050.31315680917155414No Hit
CCTGAATTGATTCAGCGAACCTCTTCTCCAAGTAAATTGATTCCAAGGTT78920.29758380951016317No Hit
CGCTCATACAAGTATTAAGAGTATACAAAAAAAGGTTAATGAAATAATTA75090.2831420204779289No Hit
CCACTCTCGACTGCCGGCGACGGCCGGGTATGGGCCCGACGCTCCAGCGC71310.26888876655055416No Hit
CCCATCACGATATGTACTGATAAAGATTACCTACACTTTTCAGTGGAGGA71180.2683985752779196No Hit
CACATATTTAGCTTTATGTGAAATGTATCACACGAATTAGTGCTCCAATC69170.2608194640625695No Hit
GTCCGTTTCAGTTCTGAGTTAACATTATTAGTATCAAGAGGGACGACGCC64550.2433988203735559No Hit
GGCACCTTATCCAAGCGTTAGGAGCATCCCTCATCGCCAGTTCTGCTTAC63290.23864773573109765No Hit
GCTCATACAAGTATTAAGAGTATACAAAAAAAGGTTAATGAAATAATTAA61800.2330293896062859No Hit
GCACCATGTAGGAACAACTCAAACAGCAAAGAAATAAATCAATGCAATTT61570.23216212812393242No Hit
CCCCAGTCAAACTCCCCACCTGGCTATGTCTCCATAGAGTCGGGAGGAAA61550.23208671408198864No Hit
CCGTTACCCGTCATAGCCATGTTAGGCCAATACCCTAACATCAAAAGCTG59900.22586505562162662No Hit
CCCGGGGCTCCCGCCGGCTTCTCCGGGATCGGTCGCGTTACCGCACTGGA59170.2231124430906786No Hit
CTACCAAATATGCTCTTACTCAATATAGCGTAATTGCTTACCGGTATTGT58790.22167957629374674No Hit
TTTTAGTCTACAAGAGATTTCTGTTCTCTTTGAGCTTGTCTTTGGACATC55700.21002810681343245No Hit
CACGATATGTACTGATAAAGATTACCTACACTTTTCAGTGGAGGAAAAAT53100.2002242813607408No Hit
CATCACGATATGTACTGATAAAGATTACCTACACTTTTCAGTGGAGGAAA53010.19988491817199378No Hit
CCGGAATCGAACTCTAATTCCCCGTTACCCGTCATAGCCATGTTAGGCCA51970.19596338799091712No Hit
CCCGAGGCAAAAAAGTCGTCTACAGTTTGTGCAATTCTTTTTTGTTGTTA51560.1944174001310696No Hit
CCCGCCTCACCGGGTCAGTGAAAAAACGATCAGAGTAGTGGTATTTCACC50750.19136313143234646No Hit
CCCGAGTGTAAGACCTATGTGAAGGAGTTCATTTGTATATATGTTACCAT49610.18706453104155088No Hit
CTACACATATTTAGCTTTATGTGAAATGTATCACACGAATTAGTGCTCCA49430.18638580466405683No Hit
CTTTTAGTCTACAAGAGATTTCTGTTCTCTTTGAGCTTGTCTTTGGACAT48340.18227573937812072No Hit
CTCTATTACACTCAAAATACTATCGGCACAGGAATATCAACCTGTTTCCC46750.17628032304359006No Hit
GTACGCTCATACAAGTATTAAGAGTATACAAAAAAAGGTTAATGAAATAA45810.17273586307223232No Hit
CCCCTTTTAGTCTACAAGAGATTTCTGTTCTCTTTGAGCTTGTCTTTGGA44190.166627325674786No Hit
CAATAGATAAGTAAAACAACTTTAAAAGTAGTGGTATTTCACTGTCGTAA42850.16157458486455262No Hit
CCCTTGTCCGTTTCAGTTCTGAGTTAACATTATTAGTATCAAGAGGGACG41880.15791700383027918No Hit
CCATGCTGTAGTATTCAAACGCGCAATGCTATATCTGTTTGCTTTGAACA41270.15561687555099385No Hit
CCCACTTGGAGTTATATTATTTGACAAACGAGCAAAATACAGCGAAGATA38110.14370145692387631No Hit
CTGAATTGATTCAGCGAACCTCTTCTCCAAGTAAATTGATTCCAAGGTTT38070.14355062883998876No Hit
CCGGATTTTCAAGGGCCAGCGAGAGCTCACCGGACGCCGCCGGAACCGCG37650.14196693395916932No Hit
CCACGCTTTCACGTTTTCTACTTTTTCTGAAAATAGAAATCAAACAAGCT37390.14098655141390018No Hit
CATCAATAGATAAGTAAAACAACTTTAAAAGTAGTGGTATTTCACTGTCG36750.13857330207169913No Hit
CGCTGATGTTCCTTTTAATAAAGAAGAAAAATAAATCCGAAGAATCATTT36330.13698960719087971No Hit
CTTGTCCGTTTCAGTTCTGAGTTAACATTATTAGTATCAAGAGGGACGAC36180.13642400187630135No Hit
GCACCTTATCCAAGCGTTAGGAGCATCCCTCATCGCCAGTTCTGCTTACC35110.13238935063230903No Hit
TACAGTTTGTGCAATTCTTTTTTGTTGTTAAAGAACTATTGCCTAGCTCA34720.1309187768144053No Hit
CACCGGGTCAGTGAAAAAACGATCAGAGTAGTGGTATTTCACCGGCGGCC34620.1305417066046864No Hit
CCCGAGTGTAAGACCTATGTGAAGGAGTTCATTTGTATGTATGTTACCAT34340.12948591001747345No Hit
GCCCTTTTTACCTATTGGGAGTTTGAGAATAGGTCAAGGAAAACGATTTC34250.12914654682872642No Hit
CTCGACTGCCGGCGACGGCCGGGTATGGGCCCGACGCTCCAGCGCCATCC33930.12793992215762592No Hit
CCCGTCATAGCCATGTTAGGCCAATACCCTAACATCAAAAGCTGATAGGT33840.1276005589688789No Hit
ATCACGATATGTACTGATAAAGATTACCTACACTTTTCAGTGGAGGAAAA33260.12541355175250923No Hit
CGTTACCCGTCATAGCCATGTTAGGCCAATACCCTAACATCAAAAGCTGA32660.12315113049419575No Hit
GTCTCCTACACATATTTAGCTTTATGTGAAATGTATCACACGAATTAGTG32150.12122807242462932No Hit
GTCCTACCAAATATGCTCTTACTCAATATAGCGTAATTGCTTACCGGTAT31630.119267307334091No Hit
CCCGTCCACTCTCGACTGCCGGCGACGGCCGGGTATGGGCCCGACGCTCC31380.11832463180979373No Hit
CCTTAACTTTCGTTCTTGATTAATGGAAGTATTTTAGGCAAATGCTTTCG29290.11044386442666852No Hit
GTCGTCTACAGTTTGTGCAATTCTTTTTTGTTGTTAAAGAACTATTGCCT28720.10829456423127072No Hit
CACGCTTTCACGTTTTCTACTTTTTCTGAAAATAGAAATCAAACAAGCTT28630.10795520104252372No Hit
CCGGCGACGGCCGGGTATGGGCCCGACGCTCCAGCGCCATCCATTTTCAG27880.1051271744696319No Hit
CTCTCGGGTCCTACCAAATATGCTCTTACTCAATATAGCGTAATTGCTTA27430.10343035852589681No Hit
GCCCCATTGGCTCCTCAGCCAAGCACATACACCAAATGTCTGAACCTGCG27210.1026008040645152No Hit
CCCCCCGCCTCACCGGGTCAGTGAAAAAACGATCAGAGTAGTGGTATTTC27130.10229914789674006No Hit
GTCGCTTCTTTGTTATAATTCCTTAAGTTAATAACTTTTTTCTTAAAAGG26840.10120564428855523No Hit
CCAATCTCAAAGAACACGACTCTAGAAGTAAAATTCCAAAAAATAGAAAG26560.10014984770134229No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)