FastQCFastQC Report
Thu 2 Nov 2023
HMLTGDMXY_n01_MHC-076.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHMLTGDMXY_n01_MHC-076.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1623916
Sequences flagged as poor quality0
Sequence length101
%GC41

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CCCCAAGCTACTCCTATTAATCGTAACTAAGCCAAAATAAGAAAAAAGAA631403.8881321447661086No Hit
CCTGAATTGATTCAGCGAACCTCTTCTCCAAGTAAATTGATTCCAAGGTT254241.5655982205976173No Hit
CTACAGTTTGTGCAATTCTTTTTTGTTGTTAAAGAACTATTGCCTAGCTC231881.4279063695412817No Hit
CTTTAATTGCTGTTCCCTCCTTGTTTCAAGGATAGTTCTTACGGCTTCGG215101.3245759017092016No Hit
CTCCAATCTCAAAGAACACGACTCTAGAAGTAAAATTCCAAAAAATAGAA201101.238364545949421No Hit
CCCGAGGCAAAAAAGTCGTCTACAGTTTGTGCAATTCTTTTTTGTTGTTA172661.0632323346774095No Hit
CCCCCAAGCTACTCCTATTAATCGTAACTAAGCCAAAATAAGAAAAAAGA154760.9530049583845469No Hit
CCATGCTGTAGTATTCAAACGCGCAATGCTATATCTGTTTGCTTTGAACA137480.8465955135610462No Hit
CCCAAGCTACTCCTATTAATCGTAACTAAGCCAAAATAAGAAAAAAGAAC120680.7431418866493095No Hit
CCCCGAGGCAAAAAAGTCGTCTACAGTTTGTGCAATTCTTTTTTGTTGTT116330.716354786823949No Hit
CTCGGGTCCTACCAAATATGCTCTTACTCAATATAGCGTAATTGCTTACC115680.7123521167351021No Hit
CCTACACATATTTAGCTTTATGTGAAATGTATCACACGAATTAGTGCTCC114460.7048394128760355No Hit
CCCCGTTACCCGTCATAGCCATGTTAGGCCAATACCCTAACATCAAAAGC113210.6971419703974836No Hit
CTCCAATCTCAAAGAACACGACTCTAGAAGTAAAATTCCAATAAATAGAA108330.6670911549612173No Hit
CTGAATTGATTCAGCGAACCTCTTCTCCAAGTAAATTGATTCCAAGGTTT94240.5803255833429808No Hit
TTTAATTGCTGTTCCCTCCTTGTTTCAAGGATAGTTCTTACGGCTTCGGA88090.5424541663485057No Hit
CCAAGCTACTCCTATTAATCGTAACTAAGCCAAAATAAGAAAAAAGAACA87910.5413457346315942No Hit
CCCGTTACCCGTCATAGCCATGTTAGGCCAATACCCTAACATCAAAAGCT85100.5240418839398098No Hit
CCCCCTTTAATTGCTGTTCCCTCCTTGTTTCAAGGATAGTTCTTACGGCT84480.520223952470448No Hit
GTCGTCTACAGTTTGTGCAATTCTTTTTTGTTGTTAAAGAACTATTGCCT83900.5166523391604No Hit
CCTTTAATTGCTGTTCCCTCCTTGTTTCAAGGATAGTTCTTACGGCTTCG78310.4822293763963161No Hit
CAAATATTTAGCTTTATGTGAAATGTATCACACGAATTAGTGCTCCAATC69400.42736200640919847No Hit
CCCTTTAATTGCTGTTCCCTCCTTGTTTCAAGGATAGTTCTTACGGCTTC62930.3875200441402141No Hit
TACAGTTTGTGCAATTCTTTTTTGTTGTTAAAGAACTATTGCCTAGCTCA61520.3788373290244077No Hit
CTCCTACACATATTTAGCTTTATGTGAAATGTATCACACGAATTAGTGCT60960.37538887479401645No Hit
CTCTATTACACTCAAAATACTATCGGCACAGGAATATCAACCTGTTTCCC60020.36960039805014544No Hit
CCCACTTGGAGTTATATTATTTGACAAACGGGCAAAATACAAATAAAGAT59920.3689846026518613No Hit
CCCGAGTGTAAGACCTATGTGAAGGAGTTCATTTGTATATATGTTACCAT56670.3489712522076265No Hit
CTACACATATTTAGCTTTATGTGAAATGTATCACACGAATTAGTGCTCCA55260.34028853709182005No Hit
CCCGAGTGTAAGACCTATGTGAAGGAGTTCATTTGTATGTATGTTACCAT54170.3335763672505228No Hit
GTACGCTCATACAAGTATTAAGAGTATACAAAAAAAGGTTAATGAAATAA51810.31904359585101694No Hit
CCCCTTTAATTGCTGTTCCCTCCTTGTTTCAAGGATAGTTCTTACGGCTT51800.3189820163111885No Hit
CCGAGGCAAAAAAGTCGTCTACAGTTTGTGCAATTCTTTTTTGTTGTTAA50640.31183878969109236No Hit
CCCGTCATAGCCATGTTAGGCCAATACCCTAACATCAAAAGCTGATAGGT49690.30598873340739297No Hit
CTACAAATATTTAGCTTTATGTGAAATGTATCACACGAATTAGTGCTCCA46880.28868488271560844No Hit
CCTACAAATATTTAGCTTTATGTGAAATGTATCACACGAATTAGTGCTCC43810.2697799639882851No Hit
AATTGATTCAGCGAACCTCTTCTCCAAGTAAATTGATTCCAAGGTTTGCT42850.2638683281647573No Hit
CTTCGGATAATTCATAAAAACGAATTACTATTACTACAAAACAGGAAATT42500.26171304427076275No Hit
GCACCATGTAGGAACAACTCAAACAGCAAAGAAATAAATCAATGCAATTT40930.25204505651770165No Hit
GCACCATTTAGGAACAACTCAAACAGCAAAGAAATAAATCAATGCAATTT39880.2455792048357181No Hit
CCAATCTCAAAGAACACGACTCTAGAAGTAAAATTCCAAAAAATAGAAAG39280.2418844324460132No Hit
CGCTCATACAAGTATTAAGAGTATACAAAAAAAGGTTAATGAAATAATTA38740.23855913729527883No Hit
GTACGCTCATACAAGTATTAAGAGTATACAAAAAAAGGTTTATGAAATAA38230.23541858076402966No Hit
GTCTACAGTTTGTGCAATTCTTTTTTGTTGTTAAAGAACTATTGCCTAGC37790.23270908101157944No Hit
CCCATCACGATATGTACTGATAAAGATTACCTACACTTTTCAGTGGAGGA36780.22648954748890954No Hit
GTCTCCTACAAATATTTAGCTTTATGTGAAATGTATCACACGAATTAGTG36520.22488847945337076No Hit
CTCTCGGGTCCTACCAAATATGCTCTTACTCAATATAGCGTAATTGCTTA36480.2246421612940571No Hit
CCCATCAATAGATAAGTAAAACAACTTTAAAAGTAGTGGTATTTCACTGT35610.21928474132898498No Hit
CGGATAATTCATAAAAACGAATTACTATTACTACAAAACAGGAAATTAAG35340.2176220937536178No Hit
TCCAATCTCAAAGAACACGACTCTAGAAGTAAAATTCCAAAAAATAGAAA35250.21706787789516208No Hit
GTTCCCTCCTTGTTTCAAGGATAGTTCTTACGGCTTCGGATAATTCATAA34510.21251099194785936No Hit
TGAATTGATTCAGCGAACCTCTTCTCCAAGTAAATTGATTCCAAGGTTTG34330.21140256023094792No Hit
GTCGCTTCTTTGTTATAATTCCTTAAGTTAATAACTTTTTTCTTAAAAGG33010.20327406097359718No Hit
GTGCAATTCTTTTTTGTTGTTAAAGAACTATTGCCTAGCTCACAGCTCAA32800.20198089063720046No Hit
CCCCCCTTTAATTGCTGTTCCCTCCTTGTTTCAAGGATAGTTCTTACGGC32350.19920981134492177No Hit
GTAGTATTCAAACGCGCAATGCTATATCTGTTTGCTTTGAACACTCTAAT31820.19594609573401578No Hit
GCTCATACAAGTATTAAGAGTATACAAAAAAAGGTTAATGAAATAATTAA31680.19508398217641798No Hit
TACAAATATTTAGCTTTATGTGAAATGTATCACACGAATTAGTGCTCCAA31590.19452976631796226No Hit
CCTCCTTGTTTCAAGGATAGTTCTTACGGCTTCGGATAATTCATAAAAAC31060.19126605070705627No Hit
CTCCTACAAATATTTAGCTTTATGTGAAATGTATCACACGAATTAGTGCT30910.19034235760963003No Hit
GAATTGATTCAGCGAACCTCTTCTCCAAGTAAATTGATTCCAAGGTTTGC29800.183507028688676No Hit
CCGTTACCCGTCATAGCCATGTTAGGCCAATACCCTAACATCAAAAGCTG29630.18246017651159296No Hit
TCCCCCTTTAATTGCTGTTCCCTCCTTGTTTCAAGGATAGTTCTTACGGC29160.17956593813965746No Hit
CGAGGCAAAAAAGTCGTCTACAGTTTGTGCAATTCTTTTTTGTTGTTAAA29120.1793196199803438No Hit
GCCCACTTGGAGTTATATTATTTGACAAACGGGCAAAATACAAATAAAGA28720.17685643838720722No Hit
CTCCTATTAATCGTAACTAAGCCAAAATAAGAAAAAAGAACAAAATTCTG28640.1763638020685799No Hit
CGCTCATACAAGTATTAAGAGTATACAAAAAAAGGTTTATGAAATAATTA27910.17186849566110562No Hit
GTCACGCTTAGTTCAAAGCCTCTCGGGTCCTACCAAATATGCTCTTACTC27300.16811214373157232No Hit
GTCTCCTACACATATTTAGCTTTATGTGAAATGTATCACACGAATTAGTG27160.16725003017397452No Hit
CACATATTTAGCTTTATGTGAAATGTATCACACGAATTAGTGCTCCAATC26670.1642326327223822No Hit
ACAAATATTTAGCTTTATGTGAAATGTATCACACGAATTAGTGCTCCAAT26050.1604147012530205No Hit
AATTGCTGTTCCCTCCTTGTTTCAAGGATAGTTCTTACGGCTTCGGATAA25640.15788994012005547No Hit
CCTATTAATCGTAACTAAGCCAAAATAAGAAAAAAGAACAAAATTCTGAC25460.156781508403144No Hit
CCGGAATCGAACTCTAATTCCCCGTTACCCGTCATAGCCATGTTAGGCCA25190.1551188608277768No Hit
CCCCAGTCAAACTCCCCACCTGGCTATGTCTCCATAGAGTCGGGAGGAAA24160.1487761682254501No Hit
CTTAAACTTCCTTGTGTTAGACACACATCGTTCCTCTAAGAAGCTTTACA23710.14600508893317143No Hit
GCTTCGGATAATTCATAAAAACGAATTACTATTACTACAAAACAGGAAAT23550.1450198162959168No Hit
TCCTACACATATTTAGCTTTATGTGAAATGTATCACACGAATTAGTGCTC23120.14237189608329495No Hit
GCTCATACAAGTATTAAGAGTATACAAAAAAAGGTTTATGAAATAATTAA23110.14231031654346654No Hit
TCTACAGTTTGTGCAATTCTTTTTTGTTGTTAAAGAACTATTGCCTAGCT22710.13984713495032994No Hit
GTCAGAAACTCGATTGATACACACTAAAAATGGACGGATCCATTACGTGT22200.1367065784190808No Hit
CTCCTTGTTTCAAGGATAGTTCTTACGGCTTCGGATAATTCATAAAAACG21900.13485919222422835No Hit
CCTATGTGAAGGAGTTCATTTGTATATATGTTACCATCTCTATTACACTC21770.13405865820645896No Hit
CAGGGACGTAGTCAGCACAATCTGATGAATCATGCTTACTAGGCATTCCT21620.13313496510903275No Hit
CGGCACAGGAATATCAACCTGTTTCCCTTTCGAGACGGGGATGAAATGCA21120.130055988117612No Hit
CCAATCTCAAAGAACACGACTCTAGAAGTAAAATTCCAATAAATAGAAAG20950.12900913594052896No Hit
GCTCCAATCTCAAAGAACACGACTCTAGAAGTAAAATTCCAAAAAATAGA20910.1287628177812153No Hit
CATCTCTATTACACTCAAAATACTATCGGCACAGGAATATCAACCTGTTT20820.12820860192275954No Hit
GGATAATTCATAAAAACGAATTACTATTACTACAAAACAGGAAATTAAGA20750.12777754514396064No Hit
CGTCTACAGTTTGTGCAATTCTTTTTTGTTGTTAAAGAACTATTGCCTAG20470.12605331802876504No Hit
TCTCGGGTCCTACCAAATATGCTCTTACTCAATATAGCGTAATTGCTTAC19790.12186590932043284No Hit
AGTTTGTGCAATTCTTTTTTGTTGTTAAAGAACTATTGCCTAGCTCACAG19610.12075747760352136No Hit
CCTTGTTTCAAGGATAGTTCTTACGGCTTCGGATAATTCATAAAAACGAA19060.11737060291295855No Hit
TCCAATCTCAAAGAACACGACTCTAGAAGTAAAATTCCAATAAATAGAAA18980.11687796659433124No Hit
CGCGCAATGCTATATCTGTTTGCTTTGAACACTCTAATTTACTCAAAGTA18970.11681638705450283No Hit
CGGCTTCGGATAATTCATAAAAACGAATTACTATTACTACAAAACAGGAA18670.11496900085965038No Hit
CTCGTCACGCTTAGTTCAAAGCCTCTCGGGTCCTACCAAATATGCTCTTA18480.11379898960291049No Hit
ACGGCTTCGGATAATTCATAAAAACGAATTACTATTACTACAAAACAGGA18150.11176686478857281No Hit
GCTATATCTGTTTGCTTTGAACACTCTAATTTACTCAAAGTAACAAGGAC18000.1108431716911466No Hit
TCTCCTACAAATATTTAGCTTTATGTGAAATGTATCACACGAATTAGTGC17910.11028895583269085No Hit
CCTATGTGAAGGAGTTCATTTGTATGTATGTTACCATCTCTATTACACTC17880.1101042172132056No Hit
CCCCAAGCTACTCCTATTAATCGTAACTAAGCCAAAATAAGAAAAAAAGA17580.10825683101835318No Hit
TTGTGCAATTCTTTTTTGTTGTTAAAGAACTATTGCCTAGCTCACAGCTC17160.10567049034555975No Hit
CCCCTTTTAGTCTACAAGAGATTTCTGTTCTCTTTGAGCTTGTCTTTGGA16540.10185255887619803No Hit
TCGGATAATTCATAAAAACGAATTACTATTACTACAAAACAGGAAATTAA16270.10018991130083084No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)