FastQCFastQC Report
Thu 2 Nov 2023
HMLTGDMXY_n01_MHC-069.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHMLTGDMXY_n01_MHC-069.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences58044788
Sequences flagged as poor quality0
Sequence length101
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CCCGAGTGTAAAGCCTATGTAAAGGAGTTCATTTATATATATGTTACCAT4632670.7981198932107393No Hit
CTCCTACACATATTTAGCTTTATGTGAAATGTATCACACGTATTAGTGCT3513550.6053170527558822No Hit
CCTACACATATTTAGCTTTATGTGAAATGTATCACACGTATTAGTGCTCC3149330.5425689555451559No Hit
CTCCAATCTCAAAGAACACGACTCGGCATGAAAAATTCCAATGAATAAAT2827750.4871669098007559No Hit
CTACAGTTTGTGCAATTCTTTTTTGTTGTTAAAGAACTATTTGCCTAGCT2627720.4527055900350605No Hit
CGCTCATACAAGTATTAAGAGTATAGCAAAGCAAAATGGAATCAGAAGAA2299780.3962078386779533No Hit
GCTCATACAAGTATTAAGAGTATAGCAAAGCAAAATGGAATCAGAAGAAA2198380.3787385699470554No Hit
CCCCGTTACCCGTCATAGCCATGTTAGGCCAATACCCTAACATCAAAAGC2179190.3754325022256951No Hit
GGCACCTTATCCAAGCGTTAGGAGCATCCCTCATCGCCAGTTCTGCTTAC2030520.34981952212488054No Hit
GTACGCTCATACAAGTATTAAGAGTATAGCAAAGCAAAATGGAATCAGAA1723430.2969138245452804No Hit
CCCCAAGCTACTCCTATTAATCGTAACTAAGCCAAAATAAGAAAAAAGAA1592820.27441223491073824No Hit
GCCCTTTTTACCTATTGGGAGTTTGAGAATAGGTCAAGGAAAACGATTTC1503300.25898966156961417No Hit
CCCGTTACCCGTCATAGCCATGTTAGGCCAATACCCTAACATCAAAAGCT1444720.24889745484125123No Hit
CCGAGTGTAAAGCCTATGTAAAGGAGTTCATTTATATATATGTTACCATC1375380.23695150717063518No Hit
GTCGTCTACAGTTTGTGCAATTCTTTTTTGTTGTTAAAGAACTATTTGCC1361910.23463088537768456No Hit
GCCGTTCCCTCCTTTTTTCAAGGATAGTCCTTACCACTTCGGATAATTCA1254900.21619512160161564No Hit
GTCTCCTACACATATTTAGCTTTATGTGAAATGTATCACACGTATTAGTG1226190.21124894107632883No Hit
CCGGAATCGAACTCTAATTCCCCGTTACCCGTCATAGCCATGTTAGGCCA1213320.20903168773740718No Hit
CCCCTTTTAGTCTACAAGAGATTTCTGTTCTCTTTGAGCTTGTCTTTGGA1184880.20413202301643343No Hit
CGCCCTTTTTACCTATTGGGAGTTTGAGAATAGGTCAAGGAAAACGATTT1136180.19574195016441442No Hit
CCCCGTTCAAGAAATTTTTTGAATTGAGTTCAACGAACCTCTTCTCCAAG1091580.18805822841492678No Hit
CTCGGGTCCTACCAAATATGCTCGTACTCAATAAAGCATAATTGCTTACC1068600.18409921662561676No Hit
CTACAGTTTGTGCAATTCTTTTTTGTTGTTAAAGAACTATTGCCTAGCTC1064150.18333256725823513No Hit
CACATATTTAGCTTTATGTGAAATGTATCACACGTATTAGTGCTCCAATC1063970.18330155672202644No Hit
CTTTTAGTCTACAAGAGATTTCTGTTCTCTTTGAGCTTGTCTTTGGACAT1057060.182111096693126No Hit
CCTGTGGTAACTTTTCAGACACCTCTATATGCACAGTAGTAAGTAATTTA1008420.17373136068650985No Hit
CCCCAGTCAAACTCCCCACCTGGCTATGTCTCCATAGAAATTGGGAGAAA997930.17192413554856983No Hit
CCCCGCCTCACCGGGTCAGTGAAAAAACGATCAGAGTAGTGGTATTTCAC964400.1661475617759169No Hit
CTCGGCATGAAAAATTCCAATGAATAAATTAAAACGAACTATGGGCCTTT957080.16488646663676332No Hit
CCCACTTGGAGTTATTTTATTTAATATGAGTGAAATACAAATTGAGTTAT918110.15817268554758093No Hit
GCACCTTATCCAAGCGTTAGGAGCATCCCTCATCGCCAGTTCTGCTTACC913020.15729577649590176No Hit
TACAGTTTGTGCAATTCTTTTTTGTTGTTAAAGAACTATTTGCCTAGCTC910320.15683061845277135No Hit
CCTCGTTCAAGATTAATAATTGCAATAATCTATCCCCATCACGATATATA899750.1550096108542941No Hit
CCACGCTTTCACGTTTTCTACTTGTTCTGAAAATAGAAATCAAACAAGCT861590.14843537717805086No Hit
GTCAGTATCAACTCGATCCTCCATCAGACTTTCGTCTGACTTCAATCTCA827640.14258644548757762No Hit
TTTTAGTCTACAAGAGATTTCTGTTCTCTTTGAGCTTGTCTTTGGACATC817610.14085846949772649No Hit
CACCGGGTCAGTGAAAAAACGATCAGAGTAGTGGTATTTCACCGGCGGCC812690.1400108481746888No Hit
CGAGTGTAAAGCCTATGTAAAGGAGTTCATTTATATATATGTTACCATCT796590.1372371279915778No Hit
CCGTTACCCGTCATAGCCATGTTAGGCCAATACCCTAACATCAAAAGCTG758120.13060948728075292No Hit
GCCACGCTTTCACGTTTTCTACTTGTTCTGAAAATAGAAATCAAACAAGC728650.12553237338036277No Hit
CCCATCACGATATATATTGATAAAGATTACCTACGCCTTTCGGCGGAGGA714180.12303947083069716No Hit
CTACACATATTTAGCTTTATGTGAAATGTATCACACGTATTAGTGCTCCA696960.12007279620006538No Hit
CTCTCGGGTCCTACCAAATATGCTCGTACTCAATAAAGCATAATTGCTTA674760.11624816340099305No Hit
GCCCACTTGGAGTTATTTTATTTAATATGAGTGAAATACAAATTGAGTTA671400.11566930005843075No Hit
AAGAAACTCATAATACTATTGGCACAGGAATATCAACCTGTTTCCCTTTT662670.11416528905230905No Hit
GTAAAATTATATTAATCAACTCCAGCGATCCTGAGAGATCTTTCGAAAAT620720.10693811130811606No Hit
CACGTATTAGTGCTCCAATCTCAAAGAACACGACTCGGCATGAAAAATTC620700.10693466569298177No Hit
CCAAGGTTTGTTATAAAGGTAAAGTGCAAAGAAAACGCACCCCGAGTGTA604970.1042246893898553No Hit
CTCCTTTTTTCAAGGATAGTCCTTACCACTTCGGATAATTCATAAAAACG598110.1030428433987906No Hit
TAAGAAACTCATAATACTATTGGCACAGGAATATCAACCTGTTTCCCTTT595670.10262247835240608No Hit
CCTGATGCGGTTGTTGGTACGCTCATACAAGTATTAAGAGTATAGCAAAG591770.1019505834012177No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)