FastQCFastQC Report
Thu 2 Nov 2023
HMLTGDMXY_n01_MHC-058.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHMLTGDMXY_n01_MHC-058.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences53253742
Sequences flagged as poor quality0
Sequence length101
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CCCGAGTGTAAAGCCTATGTAAAGGAGTTCATTTATATATATGTTACCAT4344800.8158675497395095No Hit
CCTACACATATTTAGCTTTATGTGAAATGTATCACACGTATTAGTGCTCC3563030.6690665981744531No Hit
CTCCTACACATATTTAGCTTTATGTGAAATGTATCACACGTATTAGTGCT3405640.6395118675416274No Hit
CTACAGTTTGTGCAATTCTTTTTTGTTGTTAAAGAACTATTGCCTAGCTC3239870.6083835385689892No Hit
CTCCAATCTCAAAGAACACGACTCGGCATGAAAAATTCCAATGAATAAAT3116620.5852396250389316No Hit
GCTCATACAAGTATTAAGAGTATAGCAAAGCAAAATGGAATCAGAAGAAA2251210.4227327349127879No Hit
CGCTCATACAAGTATTAAGAGTATAGCAAAGCAAAATGGAATCAGAAGAA2240060.4206389853317725No Hit
CCCCGTTACCCGTCATAGCCATGTTAGGCCAATACCCTAACATCAAAAGC1947650.3657301678443554No Hit
GGCACCTTATCCAAGCGTTAGGAGCATCCCTCATCGCCAGTTCTGCTTAC1834500.3444828346522578No Hit
CTACAGTTTGTGCAATTCTTTTTTGTTGTTAAAGAACTATTTGCCTAGCT1769000.3321832294902394No Hit
GTACGCTCATACAAGTATTAAGAGTATAGCAAAGCAAAATGGAATCAGAA1699510.3191343812046109No Hit
GTCGTCTACAGTTTGTGCAATTCTTTTTTGTTGTTAAAGAACTATTGCCT1489530.27970428819818893No Hit
GCCGTTCCCTCCTTTTTTCAAGGATAGTCCTTACCACTTCGGATAATTCA1412620.26526211059497No Hit
CCCGTTACCCGTCATAGCCATGTTAGGCCAATACCCTAACATCAAAAGCT1356770.25477458466674513No Hit
CCCCTTTTAGTCTACAAGAGATTTCTGTTCTCTTTGAGCTTGTCTTTGGA1271690.23879824257232476No Hit
CCGAGTGTAAAGCCTATGTAAAGGAGTTCATTTATATATATGTTACCATC1252540.2352022511394598No Hit
GCCCTTTTTACCTATTGGGAGTTTGAGAATAGGTCAAGGAAAACGATTTC1244870.23376197676399907No Hit
CCCCGTTCAAGAAATTTTTTGAATTGAGTTCAACGAACCTCTTCTCCAAG1196310.22464336872327206No Hit
CACATATTTAGCTTTATGTGAAATGTATCACACGTATTAGTGCTCCAATC1176970.22101169904642567No Hit
GTCTCCTACACATATTTAGCTTTATGTGAAATGTATCACACGTATTAGTG1159510.21773305620476396No Hit
TACAGTTTGTGCAATTCTTTTTTGTTGTTAAAGAACTATTGCCTAGCTCA1116410.20963972822792432No Hit
CCGGAATCGAACTCTAATTCCCCGTTACCCGTCATAGCCATGTTAGGCCA1043450.19593928253905613No Hit
CCCCAAGCTACTCCTATTAATCGTAACTAAGCCAAAATAAGAAAAAAGAA1025500.19256862738396863No Hit
GCACCTTATCCAAGCGTTAGGAGCATCCCTCATCGCCAGTTCTGCTTACC977700.18359273231916734No Hit
CTTTTAGTCTACAAGAGATTTCTGTTCTCTTTGAGCTTGTCTTTGGACAT974170.18292986810203873No Hit
CCTGTGGTAACTTTTCAGACACCTCTATATGCACAGTAGTAAGTAATTTA941520.1767988435441776No Hit
TTTTAGTCTACAAGAGATTTCTGTTCTCTTTGAGCTTGTCTTTGGACATC934980.17557076083029058No Hit
CGCCCTTTTTACCTATTGGGAGTTTGAGAATAGGTCAAGGAAAACGATTT889550.16703990491409973No Hit
CCACGCTTTCACGTTTTCTACTTGTTCTGAAAATAGAAATCAAACAAGCT859280.16135579730716387No Hit
GTCGTCTACAGTTTGTGCAATTCTTTTTTGTTGTTAAAGAACTATTTGCC847930.15922449167985228No Hit
CCCCGCCTCACCGGGTCAGTGAAAAAACGATCAGAGTAGTGGTATTTCAC823070.1545562751252297No Hit
CCCACTTGGAGTTATTTTATTTAATATGAGTGAAATACAAATTGAGTTAT820800.1541300139997674No Hit
CTACACATATTTAGCTTTATGTGAAATGTATCACACGTATTAGTGCTCCA814710.1529864323900469No Hit
CTCGGGTCCTACCAAATATGCTCGTACTCAATAAAGCATAATTGCTTACC803950.15096591709930918No Hit
GTCAGTATCAACTCGATCCTCCATCAGACTTTCGTCTGACTTCAATCTCA786800.14774548612940663No Hit
CGAGTGTAAAGCCTATGTAAAGGAGTTCATTTATATATATGTTACCATCT730350.137145292062293No Hit
CCGTTACCCGTCATAGCCATGTTAGGCCAATACCCTAACATCAAAAGCTG699050.13126777081693153No Hit
CCCCAGTCAAACTCCCCACCTGGCTATGTCTCCATAGAAATGGGAGAAAA697820.13103680113220964No Hit
CCTCGTTCAAGATTAATAATTGCAATAATCTATCCCCATCACGATATATA674800.12671410020351245No Hit
CCAAGGTTTGTTATAAAGGTAAAGTGCAAAGAAAACGCACCCCGAGTGTA633480.11895502103870935No Hit
CCAATCTCAAAGAACACGACTCGGCATGAAAAATTCCAATGAATAAATTA629610.11822831154287712No Hit
GTAAAATTATATTAATCAACTCCAGCGATCCTGAGAGATCTTTCGAAAAT616470.11576087930121418No Hit
TACAGTTTGTGCAATTCTTTTTTGTTGTTAAAGAACTATTTGCCTAGCTC607550.11408587963640188No Hit
CCTGATGCGGTTGTTGGTACGCTCATACAAGTATTAAGAGTATAGCAAAG600630.11278644043455201No Hit
CCCATCACGATATATATTGATAAAGATTACCTACGCCTTTCGGCGGAGGA598570.11239961315770072No Hit
GCCACGCTTTCACGTTTTCTACTTGTTCTGAAAATAGAAATCAAACAAGC591850.11113773000214708No Hit
CACCGGGTCAGTGAAAAAACGATCAGAGTAGTGGTATTTCACCGGCGGCC587840.11038473127390747No Hit
GTTCCCTCCTTTTTTCAAGGATAGTCCTTACCACTTCGGATAATTCATAA582470.10937635143085343No Hit
CACGTATTAGTGCTCCAATCTCAAAGAACACGACTCGGCATGAAAAATTC575070.10798677771789258No Hit
CTCCTTTTTTCAAGGATAGTCCTTACCACTTCGGATAATTCATAAAAACG550350.10334485039567737No Hit
CTCGGCATGAAAAATTCCAATGAATAAATTAAAAAGAACTATGGGCCTTT549510.10318711500123315No Hit
GTCTACAGTTTGTGCAATTCTTTTTTGTTGTTAAAGAACTATTGCCTAGC544760.10229515890169745No Hit
GCCCACTTGGAGTTATTTTATTTAATATGAGTGAAATACAAATTGAGTTA540250.10144827005771725No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)