FastQCFastQC Report
Thu 2 Nov 2023
HMLTGDMXY_n01_MHC-047.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHMLTGDMXY_n01_MHC-047.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences74325985
Sequences flagged as poor quality0
Sequence length101
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTACAGTTTGTGCAATTCTTTTTTGTTGTTAAAGAACTATTGCCTAGCTC2936270.3950529548986132No Hit
CCCGAGTGTAAAGCCTATGTAAAGGAGTTCATTTATATATATGTTACCAT2842140.3823884742328003No Hit
CTCCTACACATATTTAGCTTTATGTGAAATGTATCACACGTATTAGTGCT2230850.3001440209638661No Hit
CCTACACATATTTAGCTTTATGTGAAATGTATCACACGTATTAGTGCTCC2143500.2883917380980555No Hit
CTCCAATCTCAAAGAACACGACTCGGCATGAAAAATTCCAATGAATAAAT1747160.23506718410795363No Hit
CCCCGCCTCACCGGGTCAGTGAAAAAACGATCAGAGTAGTGGTATTTCAC1364340.18356164401992117No Hit
CCCCGTTACCCGTCATAGCCATGTTAGGCCAATACCCTAACATCAAAAGC1270010.17087025486443266No Hit
CGCTCATACAAGTATTAAGAGTATAGCAAAGCAAAATGGAATCAGAAGAA1268160.17062135133493353No Hit
GTCGTCTACAGTTTGTGCAATTCTTTTTTGTTGTTAAAGAACTATTGCCT1243500.1673035345579342No Hit
GCTCATACAAGTATTAAGAGTATAGCAAAGCAAAATGGAATCAGAAGAAA1213900.163321078085948No Hit
CACCGGGTCAGTGAAAAAACGATCAGAGTAGTGGTATTTCACCGGCGGCC1144380.15396768707471553No Hit
GGCACCTTATCCAAGCGTTAGGAGCATCCCTCATCGCCAGTTCTGCTTAC1009750.13585423724959717No Hit
GTACGCTCATACAAGTATTAAGAGTATAGCAAAGCAAAATGGAATCAGAA948040.1275516227601962No Hit
TACAGTTTGTGCAATTCTTTTTTGTTGTTAAAGAACTATTGCCTAGCTCA900010.12108954896460505No Hit
CCCGTTACCCGTCATAGCCATGTTAGGCCAATACCCTAACATCAAAAGCT847910.1140798874041158No Hit
GCCCTTTTTACCTATTGGGAGTTTGAGAATAGGTCAAGGAAAACGATTTC834760.11231065420794625No Hit
CCCCTTTTAGTCTACAAGAGATTTCTGTTCTCTTTGAGCTTGTCTTTGGA831750.11190568143832874No Hit
GCCGTTCCCTCCTTTTTTCAAGGATAGTCCTTACCACTTCGGATAATTCA830990.11180342917756152No Hit
CCGAGTGTAAAGCCTATGTAAAGGAGTTCATTTATATATATGTTACCATC805410.10836183334805452No Hit
CCCCGTTCAAGAAATTTTTTGAATTGAGTTCAACGAACCTCTTCTCCAAG799370.10754919695985193No Hit
CCCCAGTCAAACTCCCCACCTGGCTATGTCTCCATAGAAATTGGGAGAAA746280.10040633837546856No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)