FastQCFastQC Report
Thu 2 Nov 2023
HMLTGDMXY_n01_MHC-046.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHMLTGDMXY_n01_MHC-046.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences39874068
Sequences flagged as poor quality0
Sequence length101
%GC39

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTACAGTTTGTGCAATTCTTTTTTGTTGTTAAAGAACTATTGCCTAGCTC3860880.9682683994018368No Hit
CCCCAAGCTACTCCTATTAATCGTAACTAAGCCAAAATAAGAAAAAAGAA3775260.9467957972083509No Hit
GTTTAATCAGCATGGTTTCTGGCTTAAGAGGCGTTCAGCCATTATCCAAC2411030.6046611547133841No Hit
GTCGTCTACAGTTTGTGCAATTCTTTTTTGTTGTTAAAGAACTATTGCCT2227810.558711491388338No Hit
CTCCAATCTCAAAGAACACGACTCGGCATGAAAAATTCCAATGAATAAAT1660180.41635581300608704No Hit
TACAGTTTGTGCAATTCTTTTTTGTTGTTAAAGAACTATTGCCTAGCTCA1510610.37884521840109214No Hit
CCCCGTTACCCGTCATAGCCATGTTAGGCCAATACCCTAACATCAAAAGC1438510.3607632910692734No Hit
GCTCATACAAGTATTAAGAGTATAGCAAAGCAAAATGGAATCAGAAGAAA1342940.3367953327460845No Hit
CTCCAATCTCAAAGAACACGACTCTAGAAGTAAAATTCCAAAAAATAGAA1291510.32389722563546813No Hit
CATGGTTTCTGGCTTAAGAGGCGTTCAGCCATTATCCAACAGACTTAGCT1289120.3232978385852178No Hit
CTCCTACACATATTTAGCTTTATGTGAAATGTATCACACGTATTAGTGCT1226150.3075056199432674No Hit
CGCTCATACAAGTATTAAGAGTATAGCAAAGCAAAATGGAATCAGAAGAA1225960.30745796992672025No Hit
CCCATCACGATATATATTGATAAAGATTACCTACGCCTTTCGGCGGAGGA1207750.3028910920250224No Hit
CCTACACATATTTAGCTTTATGTGAAATGTATCACACGTATTAGTGCTCC1172130.2939579678702459No Hit
GTTGCCTTAAACTTCCTTGTGTTAGACACACAATGTTCCTCTAAGAAGCT1156410.2900155559748757No Hit
CCCGAGGCAAAAAAGTCGTCTACAGTTTGTGCAATTCTTTTTTGTTGTTA1150630.2885659923136009No Hit
CCCGAGTGTAAAGCCTATGTAAAGGAGTTCATTTATATATATGTTACCAT1089080.2731298948479498No Hit
CCTACACATATTTAGCTTTATGTGAAATGTATCACACGAATTAGTGCTCC1060520.26596734499223906No Hit
CACGAAAAAGGTGATAGGATTATGAATGCATCCGTAGAGGAAGCACGTAT1055760.264773586682954No Hit
CCCGTTACCCGTCATAGCCATGTTAGGCCAATACCCTAACATCAAAAGCT1021830.25626429688588587No Hit
GCCCTTTTTACCTATTGGGAGTTTGAGAATAGGTCAAGGAAAACGATTTC996240.2498465920256744No Hit
CTCCTACACATATTTAGCTTTATGTGAAATGTATCACACGAATTAGTGCT991180.24857759684815706No Hit
GACTAATTGAAGAGCAGGGAATAAAAATTCCCAACAACTTCAGTGAATTG983200.24657629615317905No Hit
CTTAAACTTCCTTGTGTTAGACACACAATGTTCCTCTAAGAAGCTTTTCA940430.23585002663886714No Hit
GCCGTTCCCTCCTTTTTTCAAGGATAGTCCTTACCACTTCGGATAATTCA920510.23085429858824535No Hit
CACGAATTAGTGCTCCAATCTCAAAGAACACGACTCTAGAAGTAAAATTC915400.22957276393268927No Hit
CCTCGTTCAAGATTAATAATTGCAATAATCTATCCCCATCACGATATATA901440.22607174166428162No Hit
CTTTAATTGCTGTTCCCTCCTTGTTTCAAGGATAGTTCTTACGGCTTCGG862420.21628593300287297No Hit
AAGAAACTCATAATACTATTGGCACAGGAATATCAACCTGTTTCCCTTTT851150.21345953465294787No Hit
GGCACATATATAATTTCTCTTTTACATTAGCAGTAAAAAAGGAAACAAAA851130.2134545188617324No Hit
GTACGCTCATACAAGTATTAAGAGTATAGCAAAGCAAAATGGAATCAGAA834560.20929893583970416No Hit
GGATTATGAATGCATCCGTAGAGGAAGCACGTATAATTTCTCTTTTACAT815730.20457656841032626No Hit
CACATATTTAGCTTTATGTGAAATGTATCACACGTATTAGTGCTCCAATC811540.20352576015068238No Hit
CTCCTTTTTTCAAGGATAGTCCTTACCACTTCGGATAATTCATAAAAACG782560.1962578786794465No Hit
CACATATTTAGCTTTATGTGAAATGTATCACACGAATTAGTGCTCCAATC777870.19508167563941559No Hit
CCATGCTATAGTATTCAAACACAGTAAATGCTTTAACTGTTTGCTTTGAA716710.17974338610246637No Hit
CCTGAATTGATTCAGCGAACCTCTTCTCCAAGTAAATTGATTCCAAGGTT711030.17831890139726903No Hit
GGATTATTTATGTACCAAAAAAAGGCACATATATAATTTCTCTTTTACAT710030.17806811183649485No Hit
CATCACGATATATATTGATAAAGATTACCTACGCCTTTCGGCGGAGGAAA704930.17678908507654648No Hit
CACGAAAAAGGTGATAGGATTATTTATGTACCAAAAAAAGGCACATATAT697600.1749507975960717No Hit
GTCCCCAAGCTACTCCTATTAATCGTAACTAAGCCAAAATAAGAAAAAAG679070.17030366703492605No Hit
ATTGTTTAATCAGCATGGTTTCTGGCTTAAGAGGCGTTCAGCCATTATCC674620.16918765348948095No Hit
GCACGTATAATTTCTCTTTTACATCAGCAGTAAAAGGATAACAAAAAAGA666920.1672565738715197No Hit
GTCTCCTACACATATTTAGCTTTATGTGAAATGTATCACACGTATTAGTG656280.16458817294488237No Hit
CCATTATCCAACAGACTTAGCTTCGCAACACTGGTTTGTCAACCAATTGC652370.16360758576225531No Hit
ATGGTTTCTGGCTTAAGAGGCGTTCAGCCATTATCCAACAGACTTAGCTT648110.1625392222333573No Hit
CTTAAACTTCCTTGTGTTAGACACACATCGTTCCTCTAAGAAGCTTTACA636830.15971031598782445No Hit
CACATATATAATTTCTCTTTTACATTAGCAGTAAAAAAGGAAACAAAAAA634810.15920372107506062No Hit
GTCTACAGTTTGTGCAATTCTTTTTTGTTGTTAAAGAACTATTGCCTAGC632750.1586870945798658No Hit
CACGATATATATTGATAAAGATTACCTACGCCTTTCGGCGGAGGAAAAGT624320.1565729385825394No Hit
CTCTATTACACTCAAAATACTATCGGCACAGGAATATCAACCTGTTTCCC606810.15218161337338343No Hit
CCCATCACGATATGTACTGATAAAGATTACCTACACTTTTCAGTGGAGGA606250.15204117121934987No Hit
CCATGCTGTAGTATTCAAACGCGCAATGCTATATCTGTTTGCTTTGAACA603820.1514317525866686No Hit
CTCCAATCTCAAAGAACACGACTCTAGAAGTAAAATTCCAAGAAATAGAA596930.14970381251293446No Hit
CGAAAAAGGTGATAGGATTATGAATGCATCCGTAGAGGAAGCACGTATAA594490.14909188598464546No Hit
AGCACGTATAATTTCTCTTTTACATCAGCAGTAAAAGGATAACAAAAAAG589410.1478178750159126No Hit
GTCAGTATCAACTCGATCCTCCATCAGACTTTCGTCTGACTTCAATCTCA588790.1476623854882326No Hit
GTAGGAGTACCACTATTCCCCGAAGGGAATTGGTGTAGTTTACATTCAGC578390.14505417405618107No Hit
CTCGGGTCCTACCAAATATGCTCGTACTCAATAAAGCATAATTGCTTACC578200.14500652403963396No Hit
GCATGGTTTCTGGCTTAAGAGGCGTTCAGCCATTATCCAACAGACTTAGC577980.14495135033626366No Hit
CTGACATCTGAATACGAATGTCCCCAAGCTACTCCTATTAATCGTAACTA574940.14418895007151014No Hit
CTCACGAACAGCAGCTCACGAAAAAGGTGATAGGATTATGAATGCATCCG568930.14268170481125728No Hit
CCAAGCTACTCCTATTAATCGTAACTAAGCCAAAATAAGAAAAAAGAACA563020.14119953850708183No Hit
CCCAAGCTACTCCTATTAATCGTAACTAAGCCAAAATAAGAAAAAAGAAC557230.1397474669501993No Hit
CCGTTACCCGTCATAGCCATGTTAGGCCAATACCCTAACATCAAAAGCTG547590.13732985558433616No Hit
CCCCGAGGCAAAAAAGTCGTCTACAGTTTGTGCAATTCTTTTTTGTTGTT546730.13711417656207037No Hit
CCCGTCATAGCCATGTTAGGCCAATACCCTAACATCAAAAGCTGATAGGT545250.13674300801212458No Hit
GTACGCTCATACAAGTATTAAGAGTATACAAAAAAAGGTTAATGAAATAA538620.13508027322419172No Hit
CGCTCATACAAGTATTAAGAGTATACAAAAAAAGGTTAATGAAATAATTA518460.13002435567898415No Hit
CTTTTTTCAAGGATAGTCCTTACCACTTCGGATAATTCATAAAAACGAAT516120.12943750810677257No Hit
CTCGTTATATATATCAGTGTAGCACGCGTGCAGCCTAGTTCATCTAAGGA514790.12910395799094287No Hit
ATCACGATATATATTGATAAAGATTACCTACGCCTTTCGGCGGAGGAAAA505000.12664872819096362No Hit
GTTCAATTTTGTTATTCCATGCTGTAGTATTCAAACGCGCAATGCTATAT501930.12587880423938685No Hit
CCGGAATCGAACTCTAATTCCCCGTTACCCGTCATAGCCATGTTAGGCCA501490.12576845683264623No Hit
GTCTCCTACACATATTTAGCTTTATGTGAAATGTATCACACGAATTAGTG489300.1227113320868089No Hit
TAAGAAACTCATAATACTATTGGCACAGGAATATCAACCTGTTTCCCTTT487990.12238279776219471No Hit
CGCCCTTTTTACCTATTGGGAGTTTGAGAATAGGTCAAGGAAAACGATTT485100.12165801593155733No Hit
CACGTATTAGTGCTCCAATCTCAAAGAACACGACTCGGCATGAAAAATTC484580.12152760535995474No Hit
CTCTGACATCTGAATACGAATGTCCCCAAGCTACTCCTATTAATCGTAAC477230.11968430208826447No Hit
GTAGTATTCAAACGCGCAATGCTATATCTGTTTGCTTTGAACACTCTAAT474490.1189971386917432No Hit
CGAGATAGTATTGTTTAATCAGCATGGTTTCTGGCTTAAGAGGCGTTCAG464740.1165519404741949No Hit
CTCCTATTAATCGTAACTAAGCCAAAATAAGAAAAAAGAACAAAAAATTT462290.11593750605029815No Hit
GTTCCCTCCTTTTTTCAAGGATAGTCCTTACCACTTCGGATAATTCATAA460230.11542087955510333No Hit
GTTTGTGCAATTCTTTTTTGTTGTTAAAGAACTATTGCCTAGCTCACAGC450040.11286533393081437No Hit
ACAGTTTGTGCAATTCTTTTTTGTTGTTAAAGAACTATTGCCTAGCTCAC449970.1128477786615602No Hit
CCAAGGTTTGTTATAAAGGTAAAGTGCAAAGAAAACGCACCCCGAGTGTA447290.11217566263868536No Hit
CTCGGCATGAAAAATTCCAATGAATAAATTAAAAAGAACTATGGGCCTTT446770.1120452520670828No Hit
GTGCAATTCTTTTTTGTTGTTAAAGAACTATTGCCTAGCTCACAGCTCAC446060.11186719147893312No Hit
GTTGCCTTAAACTTCCTTGTGTTAGACACACATCGTTCCTCTAAGAAGCT443900.11132548602766087No Hit
CGTCTACAGTTTGTGCAATTCTTTTTTGTTGTTAAAGAACTATTGCCTAG441840.11080885953246605No Hit
CTAAGAAACTCATAATACTATTGGCACAGGAATATCAACCTGTTTCCCTT435400.10919377476108032No Hit
CCTGAGGCAAAAAAGTCGTCTACAGTTTGTGCAATTCTTTTTTGTTGTTA433850.10880505094188032No Hit
CCCCGTTCAAGAAATTTTTTGAATTGAGTTCAACGAACCTCTTCTCCAAG430290.10791224010552422No Hit
CTCTCGGGTCCTACCAAATATGCTCGTACTCAATAAAGCATAATTGCTTA429950.10782697165486099No Hit
AGCATGGTTTCTGGCTTAAGAGGCGTTCAGCCATTATCCAACAGACTTAG428120.10736802675864424No Hit
GACCTGATGCGGTTGTTGGTACGCTCATACAAGTATTAAGAGTATAGCAA426560.1069767950438365No Hit
GTATAATTTCTCTTTTACATCAGCAGTAAAAGGATAACAAAAAAGAACGA425260.10665076861483007No Hit
GGGCCTTTCACCCTCTATGGGGAAGCATTCCTGCTCACTTTGCTCAAATT423160.10612411053720427No Hit
GCACATATATAATTTCTCTTTTACATTAGCAGTAAAAAAGGAAACAAAAA422360.10592347888858493No Hit
CACGAAAAAGGTGATAGGATTATTTATGTACCAAAAAAAGGTACATATAT421030.10558992877275526No Hit
CGAAAAAGGTGATAGGATTATTTATGTACCAAAAAAAGGCACATATATAA411510.10320241215418502No Hit
GCTCATACAAGTATTAAGAGTATACAAAAAAAGGTTAATGAAATAATTAA407110.1020989380867786No Hit
CTCGTTCAAGATTAATAATTGCAATAATCTATCCCCATCACGATATATAT403740.1012537772669696No Hit

[WARN]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)