FastQCFastQC Report
Thu 2 Nov 2023
HMLTGDMXY_n01_MHC-023.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHMLTGDMXY_n01_MHC-023.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences64671087
Sequences flagged as poor quality0
Sequence length101
%GC41

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTCCAATTGAGTTTTGGGAAGGTTTTAAATTCCCATCATTCCAATTACAA8564451.3243089605096634No Hit
CCCCAAGCTACTCCTATTAATCGTAACTAAGCCAAAATAAGAAAAAAGAA7895801.2209165434315339No Hit
CCTACACATATTTAGCTTTATGTGAAATGTATCACACGAATTAGTGCTCC7413101.1462773155490644No Hit
CCCCGTTACCCGTCATAGCCATGTTAGGCCAATACCCTAACATCAAAAGC5676000.8776719649075946No Hit
CTCCAATCTCAAAGAACACGACTCTAGAAGTAAAATTCCAAAAAATAGAA5671600.8769915990433252No Hit
CTCCTACACATATTTAGCTTTATGTGAAATGTATCACACGAATTAGTGCT5646500.873110421044879No Hit
CCCATCAATAGATAAGTAAAACAACTTTAAAAGTAGTGGTATTTCACTGT5004630.7738589580224622No Hit
CTACAGTTTGTGCAATTCTTTTTTGTTGTTAAAGAACTATTGCCTAGCTC4869440.7529547168427833No Hit
CCCGTTACCCGTCATAGCCATGTTAGGCCAATACCCTAACATCAAAAGCT4759380.7359362925197159No Hit
CTTTAATTGCTGTTCCCTCCTTGTTTCAAGGATAGTTCTTACGGCTTCGG4300840.665032891746508No Hit
CCCCAGTCAAACTCCCCACCTGGCTATGTCTCCATAGAGTCGGGAGGAAA4258410.6584720000144731No Hit
TTTTAGTCTACAAGAGATTTCTGTTCTCTTTGAGCTTGTCTTTGGACATC4017860.6212760889576512No Hit
CCCGAGTGTAAGACCTATGTGAAGGAGTTCATTTGTATATATGTTACCAT3954240.611438617074737No Hit
CCTGAATTGATTCAGCGAACCTCTTCTCCAAGTAAATTGATTCCAAGGTT3915180.6053988237432903No Hit
CTTAACTTTCGTTCTTGATTAATGGAAGTATTTTAGGCAAATGCTTTCGC3902400.6034226701647987No Hit
GGCACCTTATCCAAGCGTTAGGAGCATCCCTCATCGCCAGTTCTGCTTAC3730240.5768018094392011No Hit
CCCATCACGATATGTACTGATAAAGATTACCTACACTTTTCAGTGGAGGA3615140.5590040569443343No Hit
CTCGGGTCCTACCAAATATGCTCTTACTCAATATAGCGTAATTGCTTACC3460670.535118576250311No Hit
CCCCTTTTAGTCTACAAGAGATTTCTGTTCTCTTTGAGCTTGTCTTTGGA3455150.5342650263478639No Hit
CTTTTAGTCTACAAGAGATTTCTGTTCTCTTTGAGCTTGTCTTTGGACAT3321620.5136174686533412No Hit
GCACCATGTAGGAACAACTCAAACAGCAAAGAAATAAATCAATGCAATTT3242140.5013275870869466No Hit
CGCTCATACAAGTATTAAGAGTATACAAAAAAAGGTTAATGAAATAATTA3123760.483022652765988No Hit
GTCCGTTTCAGTTCTGAGTTAACATTATTAGTATCAAGAGGGACGACGCC2953810.45674352125858036No Hit
CCGTTACCCGTCATAGCCATGTTAGGCCAATACCCTAACATCAAAAGCTG2917930.451195446892674No Hit
CCCTTGTCCGTTTCAGTTCTGAGTTAACATTATTAGTATCAAGAGGGACG2854050.44131777157232566No Hit
CCCGAGGCAAAAAAGTCGTCTACAGTTTGTGCAATTCTTTTTTGTTGTTA2808250.4342357814397027No Hit
CCGGAATCGAACTCTAATTCCCCGTTACCCGTCATAGCCATGTTAGGCCA2509990.38811625355856477No Hit
CACATATTTAGCTTTATGTGAAATGTATCACACGAATTAGTGCTCCAATC2473920.38253879975761035No Hit
GTACGCTCATACAAGTATTAAGAGTATACAAAAAAAGGTTAATGAAATAA2347970.3630633268928973No Hit
CCATGCTGTAGTATTCAAACGCGCAATGCTATATCTGTTTGCTTTGAACA2285850.3534577979182567No Hit
GCTCATACAAGTATTAAGAGTATACAAAAAAAGGTTAATGAAATAATTAA2237690.3460108842766165No Hit
GTCTCCTACACATATTTAGCTTTATGTGAAATGTATCACACGAATTAGTG2199420.3400932475435275No Hit
GCACCTTATCCAAGCGTTAGGAGCATCCCTCATCGCCAGTTCTGCTTACC2036560.31491043284922676No Hit
CATCACGATATGTACTGATAAAGATTACCTACACTTTTCAGTGGAGGAAA2006960.31033342612595954No Hit
TACAGTTTGTGCAATTCTTTTTTGTTGTTAAAGAACTATTGCCTAGCTCA1977860.30583373370545014No Hit
CATCAATAGATAAGTAAAACAACTTTAAAAGTAGTGGTATTTCACTGTCG1969230.30449928883984895No Hit
CTACACATATTTAGCTTTATGTGAAATGTATCACACGAATTAGTGCTCCA1949250.30140980930164357No Hit
GTCGCTTCTTTGTTATAATTCCTTAAGTTAATAACTTTTTTCTTAAAAGG1918840.29670755340790855No Hit
CTTGTCCGTTTCAGTTCTGAGTTAACATTATTAGTATCAAGAGGGACGAC1914910.2960998629882315No Hit
CTACCAAATATGCTCTTACTCAATATAGCGTAATTGCTTACCGGTATTGT1888580.2920284918050009No Hit
CACGATATGTACTGATAAAGATTACCTACACTTTTCAGTGGAGGAAAAAT1847580.28568871897885373No Hit
CAATAGATAAGTAAAACAACTTTAAAAGTAGTGGTATTTCACTGTCGTAA1840670.2846202353147396No Hit
GTCGTCTACAGTTTGTGCAATTCTTTTTTGTTGTTAAAGAACTATTGCCT1816100.2808210104772168No Hit
CCCTTTTAGTCTACAAGAGATTTCTGTTCTCTTTGAGCTTGTCTTTGGAC1801410.27854951626218993No Hit
CTGAATTGATTCAGCGAACCTCTTCTCCAAGTAAATTGATTCCAAGGTTT1597980.24709341904211382No Hit
CTCTATTACACTCAAAATACTATCGGCACAGGAATATCAACCTGTTTCCC1576180.24372251544186974No Hit
GTCCTACCAAATATGCTCTTACTCAATATAGCGTAATTGCTTACCGGTAT1541660.23838473597946483No Hit
CCCGTCATAGCCATGTTAGGCCAATACCCTAACATCAAAAGCTGATAGGT1531300.2367827836263213No Hit
CCCCCAAGCTACTCCTATTAATCGTAACTAAGCCAAAATAAGAAAAAAGA1527640.23621684293013354No Hit
CCCCAGTCAAACTCCCCACCTGGCTATGTCTCCATAGAGTCGGGAGAAAA1522620.23544060733044428No Hit
GGATAATTCATAAAAACGAATTACTATTACTACAAAACAGGAAATTAAGA1509250.23337322287469825No Hit
CCCACTTGGAGTTATATTATTTGACAAACGAGCAAAATACAGCGAAGATA1496530.23140634701253748No Hit
CCTTAACTTTCGTTCTTGATTAATGGAAGTATTTTAGGCAAATGCTTTCG1431740.22138795966116978No Hit
CCCCGAGGCAAAAAAGTCGTCTACAGTTTGTGCAATTCTTTTTTGTTGTT1399820.2164522145731059No Hit
CCAATCTCAAAGAACACGACTCTAGAAGTAAAATTCCAAAAAATAGAAAG1385670.21426421980505755No Hit
CGGATAATTCATAAAAACGAATTACTATTACTACAAAACAGGAAATTAAG1375550.21269937831723784No Hit
CCACGATTTTCTACTCATAAAAGGATACTAAAAAGAAGAAGTTCTTTTCT1334300.20632094833971168No Hit
CTCCACTTCAGTCTTCAAAGTTCTCATTTGAATAGTTGCTACTTCCACCA1327060.20520143723577738No Hit
ATCACGATATGTACTGATAAAGATTACCTACACTTTTCAGTGGAGGAAAA1314760.20329950538793326No Hit
CCACGCTTTCACGTTTTCTACTTTTTCTGAAAATAGAAATCAAACAAGCT1306200.20197588452471812No Hit
AATTGATTCAGCGAACCTCTTCTCCAAGTAAATTGATTCCAAGGTTTGCT1294530.20017136869834892No Hit
CACGATTTTCTACTCATAAAAGGATACTAAAAAGAAGAAGTTCTTTTCTT1293930.20007859153503946No Hit
CGTTACCCGTCATAGCCATGTTAGGCCAATACCCTAACATCAAAAGCTGA1279790.19789214305304625No Hit
CCATCAATAGATAAGTAAAACAACTTTAAAAGTAGTGGTATTTCACTGTC1270220.19641234729826018No Hit
CCAAGCTACTCCTATTAATCGTAACTAAGCCAAAATAAGAAAAAAGAACA1257250.19440681428472048No Hit
CCCAAGCTACTCCTATTAATCGTAACTAAGCCAAAATAAGAAAAAAGAAC1250180.19331359004372387No Hit
CCGAGGCAAAAAAGTCGTCTACAGTTTGTGCAATTCTTTTTTGTTGTTAA1239290.19162968452965698No Hit
CCATCACGATATGTACTGATAAAGATTACCTACACTTTTCAGTGGAGGAA1191030.1841673080274652No Hit
CCCCTTTAATTGCTGTTCCCTCCTTGTTTCAAGGATAGTTCTTACGGCTT1184040.18308645407490987No Hit
CCCCGCCTCACCGGGTCAGTGAAAAAACGATCAGAGTAGTGGTATTTCAC1182750.18288698317379448No Hit
GTCAAACTCCCCACCTGGCTATGTCTCCATAGAGTCGGGAGGAAAGTGAA1159360.1792702200907803No Hit
CGCTGATGTTCCTTTTAATAAAGAAGAAAAATAAATCCGAAGAATCATTT1151500.17805483925142623No Hit
GCTACTTCCACCAAGATCTGCACTAGGGATATGTTCCACGAAAACTCACG1149530.17775022089856013No Hit
TTTAATTGCTGTTCCCTCCTTGTTTCAAGGATAGTTCTTACGGCTTCGGA1140160.17630135086487722No Hit
GTAGTATTCAAACGCGCAATGCTATATCTGTTTGCTTTGAACACTCTAAT1137450.17588230734392946No Hit
CTCTCGGGTCCTACCAAATATGCTCTTACTCAATATAGCGTAATTGCTTA1121830.17346700852577288No Hit
GCCCTTTTTACCTATTGGGAGTTTGAGAATAGGTCAAGGAAAACGATTTC1115630.17250831117157503No Hit
CCGAGTGTAAGACCTATGTGAAGGAGTTCATTTGTATATATGTTACCATC1111580.17188206531923608No Hit
GTAAAATTATATTAGTCAACTCCAGCGATCCTGAGAGATCTTTCGAAAAT1102270.17044247300188414No Hit
GTGCAATTCTTTTTTGTTGTTAAAGAACTATTGCCTAGCTCACAGCTCAA1058850.16372849895038877No Hit
GAATTGATTCAGCGAACCTCTTCTCCAAGTAAATTGATTCCAAGGTTTGC1019200.1575974747416879No Hit
GCTTCGGATAATTCATAAAAACGAATTACTATTACTACAAAACAGGAAAT981180.15171849515997773No Hit
CCTTTAATTGCTGTTCCCTCCTTGTTTCAAGGATAGTTCTTACGGCTTCG970810.15011499652077906No Hit
CCCAGTCAAACTCCCCACCTGGCTATGTCTCCATAGAGTCGGGAGGAAAG961720.14870942249664057No Hit
CTCCTTGTTTCAAGGATAGTTCTTACGGCTTCGGATAATTCATAAAAACG961420.14866303391498584No Hit
CCCCCTTTAATTGCTGTTCCCTCCTTGTTTCAAGGATAGTTCTTACGGCT959540.14837233213661616No Hit
GCTCCCTCTCCGGAATCGAACTCTAATTCCCCGTTACCCGTCATAGCCAT954360.1475713559600444No Hit
CTCAGAGCCAATCCTTTTCCCGAAGTTACGGATCTATTTTGCCGACTTCC945480.14619825394306424No Hit
CCCTTTAATTGCTGTTCCCTCCTTGTTTCAAGGATAGTTCTTACGGCTTC923440.14279023947749633No Hit
CTTCGGATAATTCATAAAAACGAATTACTATTACTACAAAACAGGAAATT923030.14272684174923486No Hit
CTCCCTTAACTTTCGTTCTTGATTAATGGAAGTATTTTAGGCAAATGCTT909410.14062080014210987No Hit
CCTTGTCCGTTTCAGTTCTGAGTTAACATTATTAGTATCAAGAGGGACGA885630.1369437319029445No Hit
CACGCTTTCACGTTTTCTACTTTTTCTGAAAATAGAAATCAAACAAGCTT866930.13405217697979932No Hit
CCTCACGGTACTTGTTTGCTATCGGTCTCCTACACATATTTAGCTTTATG859750.13294194359219602No Hit
ATCAATAGATAAGTAAAACAACTTTAAAAGTAGTGGTATTTCACTGTCGT859030.13283061099622465No Hit
CCTTGTTTCAAGGATAGTTCTTACGGCTTCGGATAATTCATAAAAACGAA845390.1307214768169893No Hit
TACACATATTTAGCTTTATGTGAAATGTATCACACGAATTAGTGCTCCAA841580.13013234182997419No Hit
CTTCCGTCAATTCTTTTAACTTTCTCGCTTGCGCGAATACTCGCCCCAGA826840.12785311618467152No Hit
GTCACGCTTAGTTCAAAGCCTCTCGGGTCCTACCAAATATGCTCTTACTC822150.1271279080248025No Hit
CCTACCAAATATGCTCTTACTCAATATAGCGTAATTGCTTACCGGTATTG814610.12596200833921348No Hit
CTTAAACTTCCTTGTGTTAGACACACATCGTTCCTCTAAGAAGCTTTACA784600.12132160388768477No Hit
ACACATATTTAGCTTTATGTGAAATGTATCACACGAATTAGTGCTCCAAT781240.12080205177315173No Hit
CCCCATCAATAGATAAGTAAAACAACTTTAAAAGTAGTGGTATTTCACTG778250.12033971224265953No Hit
CGCTTCTTTGTTATAATTCCTTAAGTTAATAACTTTTTTCTTAAAAGGCT770710.11917381255707052No Hit
ACCAAATATGCTCTTACTCAATATAGCGTAATTGCTTACCGGTATTGTAT764660.11823830949370003No Hit
CTCCCGTCCACTCTCGACTGCCGGCGACGGCCGGGTATGGGCCCGACGCT753940.1165806908425708No Hit
CCTGTGGTAACTTTTCAGACACCTCTATATGCACAGTAGGAAGATGAATT745350.11525243112119023No Hit
GTTCCCTCCTTGTTTCAAGGATAGTTCTTACGGCTTCGGATAATTCATAA744600.11513645966705337No Hit
GCCACTTATTAATTCAATTAATATGAATACCCGGCGAACCGAGTAATATA743270.1149308036217174No Hit
TTAACTTTCGTTCTTGATTAATGGAAGTATTTTAGGCAAATGCTTTCGCA737580.11405096685633256No Hit
AGGCACCTTATCCAAGCGTTAGGAGCATCCCTCATCGCCAGTTCTGCTTA736710.11391643996953382No Hit
CTCTTACTCAATATAGCGTAATTGCTTACCGGTATTGTATGCGCCGACAG735810.11377727422456964No Hit
CGGCTTCGGATAATTCATAAAAACGAATTACTATTACTACAAAACAGGAA735020.11365511762621217No Hit
CAGCGATCCTGAGAGATCTTTCGAAAATAACCAGCTAATAGGCTTTTCGA731010.11303505691809385No Hit
GGTCCTACCAAATATGCTCTTACTCAATATAGCGTAATTGCTTACCGGTA724720.11206244298939957No Hit
TCCAATCTCAAAGAACACGACTCTAGAAGTAAAATTCCAAAAAATAGAAA716650.11081459014288719No Hit
CTCCGGAATCGAACTCTAATTCCCCGTTACCCGTCATAGCCATGTTAGGC710470.10985898536079965No Hit
CGGTCTCCTACACATATTTAGCTTTATGTGAAATGTATCACACGAATTAG709250.10967033846207039No Hit
CCTCCTTGTTTCAAGGATAGTTCTTACGGCTTCGGATAATTCATAAAAAC708690.10958374644298155No Hit
ATTACACTCAAAATACTATCGGCACAGGAATATCAACCTGTTTCCCTTTC695110.10748388998007719No Hit
CCCCATCACGATATGTACTGATAAAGATTACCTACACTTTTCAGTGGAGG691820.10697516186793025No Hit
CCTGTGGTAACTTTTCAGACACCTCTATATGCACAGTAGGAAGATAAATT691610.10694268986077193No Hit
GTCCACTCTCGACTGCCGGCGACGGCCGGGTATGGGCCCGACGCTCCAGC689720.10665044179634711No Hit
CCTTTTAGTCTACAAGAGATTTCTGTTCTCTTTGAGCTTGTCTTTGGACA680210.10517992375789198No Hit
TCCTACACATATTTAGCTTTATGTGAAATGTATCACACGAATTAGTGCTC678200.10486912026080525No Hit
CCTTATCCAAGCGTTAGGAGCATCCCTCATCGCCAGTTCTGCTTACCAAA664210.10270586606963943No Hit
CTCGGGTCCTACCAAATATGCTCTTACTCAACATAGCGTAATTGCTTACC659610.10199457448426683No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)