FastQCFastQC Report
Thu 2 Nov 2023
HMLTGDMXY_n01_MHC-022.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHMLTGDMXY_n01_MHC-022.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1875975
Sequences flagged as poor quality0
Sequence length101
%GC40

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CCTGAATTGATTCAGCGAACCTCTTCTCCAAGTAAATTGATTCCAAGGTT186290.9930302909153907No Hit
CTTTAATTGCTGTTCCCTCCTTGTTTCAAGGATAGTTCTTACGGCTTCGG121310.6466504084542704No Hit
CCCCGTTACCCGTCATAGCCATGTTAGGCCAATACCCTAACATCAAAAGC119980.63956076173723No Hit
CTACAGTTTGTGCAATTCTTTTTTGTTGTTAAAGAACTATTGCCTAGCTC112140.5977691600367808No Hit
CCCGAGTGTAAGACCTATGTGAAGGAGTTCATTTGTATATATGTTACCAT105370.5616812590786124No Hit
GTACGCTCATACAAGTATTAAGAGTATACAAAAAAAGGTTAATGAAATAA101270.5398259571689389No Hit
GCTCATACAAGTATTAAGAGTATACAAAAAAAGGTTAATGAAATAATTAA95040.5066165593891176No Hit
CCCCAAGCTACTCCTATTAATCGTAACTAAGCCAAAATAAGAAAAAAGAA92550.49334346140007196No Hit
CGCTCATACAAGTATTAAGAGTATACAAAAAAAGGTTAATGAAATAATTA83490.44504857474113463No Hit
CCCCCTTTAATTGCTGTTCCCTCCTTGTTTCAAGGATAGTTCTTACGGCT71620.38177481043190875No Hit
CCTACACATATTTAGCTTTATGTGAAATGTATCACACGAATTAGTGCTCC67410.3593331467636829No Hit
CTGAATTGATTCAGCGAACCTCTTCTCCAAGTAAATTGATTCCAAGGTTT63990.34110262663415025No Hit
CCCGTTACCCGTCATAGCCATGTTAGGCCAATACCCTAACATCAAAAGCT63770.3399299031170458No Hit
CCCCTTTAATTGCTGTTCCCTCCTTGTTTCAAGGATAGTTCTTACGGCTT59690.31818121243619985No Hit
CTCCTACACATATTTAGCTTTATGTGAAATGTATCACACGAATTAGTGCT58120.30981223097322724No Hit
GTCGTCTACAGTTTGTGCAATTCTTTTTTGTTGTTAAAGAACTATTGCCT56740.3024560561841176No Hit
CCCGAGGCAAAAAAGTCGTCTACAGTTTGTGCAATTCTTTTTTGTTGTTA52120.2778288623249244No Hit
CCCTTTAATTGCTGTTCCCTCCTTGTTTCAAGGATAGTTCTTACGGCTTC52050.27745572302402755No Hit
CCTTTAATTGCTGTTCCCTCCTTGTTTCAAGGATAGTTCTTACGGCTTCG51570.27489705353216326No Hit
CTCTCGGGTCCTACCAAATATGCTCTTACTCAATATAGCGTAATTGCTTA49220.26237023414491134No Hit
CCCCGAGGCAAAAAAGTCGTCTACAGTTTGTGCAATTCTTTTTTGTTGTT48230.2570929783179413No Hit
AAAAAAGTCGTCTACAGTTTGTGCAATTCTTTTTTGTTGTTAAAGAACTA42850.22841455776329642No Hit
CTCCAATCTCAAAGAACACGACTCTAGAAGTAAAATTCCAAAAAATAGAA41140.21929929769853007No Hit
CTCGGGTCCTACCAAATATGCTCTTACTCAATATAGCGTAATTGCTTACC40100.21375551379949095No Hit
GCTTCGGATAATTCATAAAAACGAATTACTATTACTACAAAACAGGAAAT39510.2106104825490745No Hit
CTCCTTGTTTCAAGGATAGTTCTTACGGCTTCGGATAATTCATAAAAACG36420.1941390476951985No Hit
CTTCGGATAATTCATAAAAACGAATTACTATTACTACAAAACAGGAAATT34810.18555684377457055No Hit
GTCTCCTACACATATTTAGCTTTATGTGAAATGTATCACACGAATTAGTG34770.1853436213169152No Hit
CTCTATTACACTCAAAATACTATCGGCACAGGAATATCAACCTGTTTCCC34680.18486387078719066No Hit
CCCCTTTTGAGAAGAGTTATAAAACCAGTTGTCCCCTCGTTAGAGTGGGG34650.18470395394394915No Hit
CTCACGAAAAAGGTGATAGGATTATGAATGCATCCGTAGAGGAAGCACGT31580.16833913031890085No Hit
GAATTGATTCAGCGAACCTCTTCTCCAAGTAAATTGATTCCAAGGTTTGC30320.16162262290275725No Hit
GTCTACAGTTTGTGCAATTCTTTTTTGTTGTTAAAGAACTATTGCCTAGC30270.16135609483068805No Hit
CCTCCTTGTTTCAAGGATAGTTCTTACGGCTTCGGATAATTCATAAAAAC30180.1608763443009635No Hit
CCCCCAAGCTACTCCTATTAATCGTAACTAAGCCAAAATAAGAAAAAAGA29790.15879742533882382No Hit
CACATATTTAGCTTTATGTGAAATGTATCACACGAATTAGTGCTCCAATC28880.15394661442716454No Hit
GTAAGACCTATGTGAAGGAGTTCATTTGTATATATGTTACCATCTCTATT28840.1537333919695092No Hit
CACGAAAAAGGTGATAGGATTATGAATGCATCCGTAGAGGAAGCACGTAT27770.1480296912272285No Hit
CTCCTATTAATCGTAACTAAGCCAAAATAAGAAAAAAGAACAAAATTCTG27680.14754994069750396No Hit
CCCCTTTTAGTCTACAAGAGATTTCTGTTCTCTTTGAGCTTGTCTTTGGA26440.14094004451018805No Hit
AATTGATTCAGCGAACCTCTTCTCCAAGTAAATTGATTCCAAGGTTTGCT26110.1391809592345314No Hit
GACCTGATGCGGTTGTTGGTACGCTCATACAAGTATTAAGAGTATACAAA26050.13886112554804833No Hit
CCCGTCATAGCCATGTTAGGCCAATACCCTAACATCAAAAGCTGATAGGT25670.1368355122003225No Hit
CATCTCTATTACACTCAAAATACTATCGGCACAGGAATATCAACCTGTTT25180.13422353709404444No Hit
GCACCATGTAGGAACAACTCAAACAGCAAAGAAATAAATCAATGCAATTT24190.12894628126707447No Hit
CCATGCTGTAGTATTCAAACGCGCAATGCTATATCTGTTTGCTTTGAACA24040.1281466970508669No Hit
GTTCCCTCCTTGTTTCAAGGATAGTTCTTACGGCTTCGGATAATTCATAA24030.12809339143645304No Hit
GTTAAGAAAGCACCATGTAGGAACAACTCAAACAGCAAAGAAATAAATCA23230.123828942283346No Hit
CGGATAATTCATAAAAACGAATTACTATTACTACAAAACAGGAAATTAAG23090.12308266368155225No Hit
GTTGGTACGCTCATACAAGTATTAAGAGTATACAAAAAAAGGTTAATGAA23010.12265621876624155No Hit
CTTTTTTGTTGTTAAAGAACTATTGCCTAGCTCACAGCTCAAATAGTCCA20980.11183517904023242No Hit
CCGAGTGTAAGACCTATGTGAAGGAGTTCATTTGTATATATGTTACCATC20500.10927650954836818No Hit
CTCATACAAGTATTAAGAGTATACAAAAAAAGGTTAATGAAATAATTAAA20110.10719759058622849No Hit
CTACACATATTTAGCTTTATGTGAAATGTATCACACGAATTAGTGCTCCA20060.10693106251415929No Hit
GCACGTATAATTTCTCTTTTACATCAGCAGTAAAAGGATAACAAAAAAGA20040.10682445128533163No Hit
GCCCCCAAGCTACTCCTATTAATCGTAACTAAGCCAAAATAAGAAAAAAG19350.10314636389077679No Hit
CTCTGACATCTGAATACAAATGCCCCCAAGCTACTCCTATTAATCGTAAC19260.10266661336105225No Hit
TACAGTTTGTGCAATTCTTTTTTGTTGTTAAAGAACTATTGCCTAGCTCA19250.10261330774663843No Hit
CCCCTTTTGAGAAGAGTTATAAAACCAGTTGTCCCCTCGTTAGTGTGGGG18960.10106744492863712No Hit
GTCACGCTTAGTTCAAAGCCTCTCGGGTCCTACCAAATATGCTCTTACTC18950.10101413931422328No Hit
GTCCTACCAAATATGCTCTTACTCAATATAGCGTAATTGCTTACCGGTAT18870.10058769439891257No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)