FastQCFastQC Report
Thu 2 Nov 2023
HMLTGDMXY_n01_MHC-009.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHMLTGDMXY_n01_MHC-009.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2508506
Sequences flagged as poor quality0
Sequence length101
%GC41

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CCTGAATTGATTCAGCGAACCTCTTCTCCAAGTAAATTGATTCCAAGGTT366661.4616668247953164No Hit
CCCCAAGCTACTCCTATTAATCGTAACTAAGCCAAAATAAGAAAAAAGAA353731.4101222002259513No Hit
CCTACACATATTTAGCTTTATGTGAAATGTATCACACGAATTAGTGCTCC315431.2574416804265167No Hit
CTACAGTTTGTGCAATTCTTTTTTGTTGTTAAAGAACTATTGCCTAGCTC308361.22925757403012No Hit
CTCCTACACATATTTAGCTTTATGTGAAATGTATCACACGAATTAGTGCT274751.0952734416421568No Hit
CTTTAATTGCTGTTCCCTCCTTGTTTCAAGGATAGTTCTTACGGCTTCGG273051.088496499510067No Hit
CTCGGGTCCTACCAAATATGCTCTTACTCAATATAGCGTAATTGCTTACC210140.8377097762572623No Hit
GCACCATGTAGGAACAACTCAAACAGCAAAGAAATAAATCAATGCAATTT205400.8188140670183766No Hit
CTCCAATCTCAAAGAACACGACTCTAGAAGTAAAATTCCAAAAAATAGAA195000.7773551269161804No Hit
CCCCGTTACCCGTCATAGCCATGTTAGGCCAATACCCTAACATCAAAAGC191810.7646383943271414No Hit
CCCGAGGCAAAAAAGTCGTCTACAGTTTGTGCAATTCTTTTTTGTTGTTA187260.7465001080324304No Hit
CTCCAATCTCAAAGAACACGACTCTAGAAGTAAAATTCCAAGAAATAGAA185880.7409988255957929No Hit
GTACGCTCATACAAGTATTAAGAGTATACAAAAAAAGGTTAATGAAATAA166360.6631835841732091No Hit
GTCGTCTACAGTTTGTGCAATTCTTTTTTGTTGTTAAAGAACTATTGCCT148300.5911885401111259No Hit
CGCTCATACAAGTATTAAGAGTATACAAAAAAAGGTTAATGAAATAATTA147210.586843324273492No Hit
CCCGTTACCCGTCATAGCCATGTTAGGCCAATACCCTAACATCAAAAGCT132120.5266879967598244No Hit
CCCCGAGGCAAAAAAGTCGTCTACAGTTTGTGCAATTCTTTTTTGTTGTT125820.5015734465056093No Hit
CTGAATTGATTCAGCGAACCTCTTCTCCAAGTAAATTGATTCCAAGGTTT121760.48538851411955963No Hit
CCCGAGTGTAAGACCTATGTGAAGGAGTTCATTTGTATATATGTTACCAT115990.4623867752359372No Hit
CTCTATTACACTCAAAATACTATCGGCACAGGAATATCAACCTGTTTCCC107630.4290601656922487No Hit
CTCTCGGGTCCTACCAAATATGCTCTTACTCAATATAGCGTAATTGCTTA105870.4220440373672616No Hit
TACAGTTTGTGCAATTCTTTTTTGTTGTTAAAGAACTATTGCCTAGCTCA101380.40414493726544803No Hit
CCCCCTTTAATTGCTGTTCCCTCCTTGTTTCAAGGATAGTTCTTACGGCT98310.3919065770622035No Hit
GCTCATACAAGTATTAAGAGTATACAAAAAAAGGTTAATGAAATAATTAA97690.3894349864022649No Hit
CACATATTTAGCTTTATGTGAAATGTATCACACGAATTAGTGCTCCAATC94700.3775155411228835No Hit
CCCCCAAGCTACTCCTATTAATCGTAACTAAGCCAAAATAAGAAAAAAGA88700.3535969218331549No Hit
CCCCTTTAATTGCTGTTCCCTCCTTGTTTCAAGGATAGTTCTTACGGCTT87920.35048750132549017No Hit
GTCTCCTACACATATTTAGCTTTATGTGAAATGTATCACACGAATTAGTG86800.3460226923914075No Hit
CCCCAGTCAAACTCCCCACCTGGCTATGTCTCCATAGAGTCGGGAGGAAA85650.3414382903608762No Hit
CCCCTTTTAGTCTACAAGAGATTTCTGTTCTCTTTGAGCTTGTCTTTGGA84620.33733226071613936No Hit
CTACACATATTTAGCTTTATGTGAAATGTATCACACGAATTAGTGCTCCA83910.3345018907668548No Hit
CCATGCTGTAGTATTCAAACGCGCAATGCTATATCTGTTTGCTTTGAACA76670.30564009015724897No Hit
GTCACGCTTAGTTCAAAGCCTCTCGGGTCCTACCAAATATGCTCTTACTC73090.29136864731437756No Hit
CCGAGGCAAAAAAGTCGTCTACAGTTTGTGCAATTCTTTTTTGTTGTTAA70580.28136269157817445No Hit
CCCATCACGATATGTACTGATAAAGATTACCTACACTTTTCAGTGGAGGA69770.2781336779740611No Hit
CCTTTAATTGCTGTTCCCTCCTTGTTTCAAGGATAGTTCTTACGGCTTCG69760.27809381360857816No Hit
CCCGTCATAGCCATGTTAGGCCAATACCCTAACATCAAAAGCTGATAGGT67730.2700013474155533No Hit
CCCTTTAATTGCTGTTCCCTCCTTGTTTCAAGGATAGTTCTTACGGCTTC66980.26701152000433725No Hit
AATTGATTCAGCGAACCTCTTCTCCAAGTAAATTGATTCCAAGGTTTGCT64510.2571650217300656No Hit
CCGTTACCCGTCATAGCCATGTTAGGCCAATACCCTAACATCAAAAGCTG61000.24317262944557436No Hit
TTTAATTGCTGTTCCCTCCTTGTTTCAAGGATAGTTCTTACGGCTTCGGA58210.23205047147585056No Hit
CTTTTAGTCTACAAGAGATTTCTGTTCTCTTTGAGCTTGTCTTTGGACAT58180.23193087837940193No Hit
GAATTGATTCAGCGAACCTCTTCTCCAAGTAAATTGATTCCAAGGTTTGC55090.21961278944519166No Hit
GCTTCGGATAATTCATAAAAACGAATTACTATTACTACAAAACAGGAAAT55070.21953306071422593No Hit
GTCTACAGTTTGTGCAATTCTTTTTTGTTGTTAAAGAACTATTGCCTAGC54340.2166229620339756No Hit
GTCCTACCAAATATGCTCTTACTCAATATAGCGTAATTGCTTACCGGTAT54250.21626418274462966No Hit
TCTCTATTACACTCAAAATACTATCGGCACAGGAATATCAACCTGTTTCC51910.20693592122163548No Hit
GTTCCCTCCTTGTTTCAAGGATAGTTCTTACGGCTTCGGATAATTCATAA51300.20450419492717975No Hit
TTTTAGTCTACAAGAGATTTCTGTTCTCTTTGAGCTTGTCTTTGGACATC50120.1998001998001998No Hit
CATCTCTATTACACTCAAAATACTATCGGCACAGGAATATCAACCTGTTT49430.19704955858188103No Hit
GTCGCTTCTTTGTTATAATTCCTTAAGTTAATAACTTTTTTCTTAAAAGG47370.1888374992924075No Hit
GGCACCTTATCCAAGCGTTAGGAGCATCCCTCATCGCCAGTTCTGCTTAC46950.1871631959421265No Hit
CCCAAGCTACTCCTATTAATCGTAACTAAGCCAAAATAAGAAAAAAGAAC46280.18449228345477348No Hit
AGAAAATCTAAGAATTTCACCTCTGACATCTGAATACAAATGCCCCCAAG45660.18202069279483485No Hit
GCCACTTATTAATTCAATTAATATGAATACCCGGCGAACCGAGTAATATA45340.18074503309938267No Hit
CCCACTTGGAGTTATATTATTTGACAAACGAGCAAAATACAGCGAAGATA44840.1787518148252386No Hit
CTTAACTTTCGTTCTTGATTAATGGAAGTATTTTAGGCAAATGCTTTCGC44650.17799439188106386No Hit
CGGATAATTCATAAAAACGAATTACTATTACTACAAAACAGGAAATTAAG44560.17763561259171795No Hit
CTTCGGATAATTCATAAAAACGAATTACTATTACTACAAAACAGGAAATT44360.1768383252820603No Hit
CTACCAAATATGCTCTTACTCAATATAGCGTAATTGCTTACCGGTATTGT43730.17432687025663882No Hit
CCCATCAATAGATAAGTAAAACAACTTTAAAAGTAGTGGTATTTCACTGT42700.17022084061190207No Hit
TCCTACACATATTTAGCTTTATGTGAAATGTATCACACGAATTAGTGCTC42000.1674303350281004No Hit
GTTAAGAAAGCACCATGTAGGAACAACTCAAACAGCAAAGAAATAAATCA41880.1669519626423058No Hit
CTCCTTGTTTCAAGGATAGTTCTTACGGCTTCGGATAATTCATAAAAACG41050.1636432203072267No Hit
TGAATTGATTCAGCGAACCTCTTCTCCAAGTAAATTGATTCCAAGGTTTG39060.15571021157613335No Hit
GTGCAATTCTTTTTTGTTGTTAAAGAACTATTGCCTAGCTCACAGCTCAA38910.15511224609389015No Hit
GGGTTAAGAAAGCACCATGTAGGAACAACTCAAACAGCAAAGAAATAAAT37850.15088662335270475No Hit
GGTCCTACCAAATATGCTCTTACTCAATATAGCGTAATTGCTTACCGGTA37350.1488934050785607No Hit
GGATAATTCATAAAAACGAATTACTATTACTACAAAACAGGAAATTAAGA37040.1476576097485914No Hit
CCAAGCTACTCCTATTAATCGTAACTAAGCCAAAATAAGAAAAAAGAACA36750.14650154314958783No Hit
CTCGTCACGCTTAGTTCAAAGCCTCTCGGGTCCTACCAAATATGCTCTTA36580.14582384893637887No Hit
GGTTAAGAAAGCACCATGTAGGAACAACTCAAACAGCAAAGAAATAAATC36550.1457042558399302No Hit
GTCAGTATCAATTCGATCTTCCATCAGACTTTCGTCTGACTTCAATCTCG36250.1445083248754438No Hit
GTTGCCTTAAACTTCCTTGTGTTAGACACACATCGTTCCTCTAAGAAGCT36110.14395022375868347No Hit
CCTCCTTGTTTCAAGGATAGTTCTTACGGCTTCGGATAATTCATAAAAAC35550.14171781929164212No Hit
CATCACGATATGTACTGATAAAGATTACCTACACTTTTCAGTGGAGGAAA33720.1344226404082749No Hit
TCTACAGTTTGTGCAATTCTTTTTTGTTGTTAAAGAACTATTGCCTAGCT33660.13418345421537758No Hit
CGTCTACAGTTTGTGCAATTCTTTTTTGTTGTTAAAGAACTATTGCCTAG33350.1329476588854083No Hit
CTTAAACTTCCTTGTGTTAGACACACATCGTTCCTCTAAGAAGCTTTACA32710.13039633949450388No Hit
GTGGGTTAAGAAAGCACCATGTAGGAACAACTCAAACAGCAAAGAAATAA32660.1301970176670895No Hit
TAAGAAAGCACCATGTAGGAACAACTCAAACAGCAAAGAAATAAATCAAT32470.12943959472291475No Hit
CCTATGTGAAGGAGTTCATTTGTATATATGTTACCATCTCTATTACACTC31730.12648963167718155No Hit
CCCACTTGGAGTTATATTATTTGACAAACGGGCAAAATACAAATAAAGAT31230.1244964134030375No Hit
CTCCTATTAATCGTAACTAAGCCAAAATAAGAAAAAAGAACAAAATTCTG31200.12437682030658886No Hit
CCCTTTTAGTCTACAAGAGATTTCTGTTCTCTTTGAGCTTGTCTTTGGAC31070.1238585835553114No Hit
TACACATATTTAGCTTTATGTGAAATGTATCACACGAATTAGTGCTCCAA30840.12294170314920513No Hit
GCTGTTCCCTCCTTGTTTCAAGGATAGTTCTTACGGCTTCGGATAATTCA30610.12202482274309887No Hit
CACGAATTAGTGCTCCAATCTCAAAGAACACGACTCTAGAAGTAAAATTC29860.1190349953318828No Hit
ACGGCTTCGGATAATTCATAAAAACGAATTACTATTACTACAAAACAGGA29310.11684245523032434No Hit
CCCCTTTTGAGAAGAGTTATAAAACCAGTTGTCCCCTCGTTAGAGTGGGG28960.1154472024384235No Hit
CGGCTTCGGATAATTCATAAAAACGAATTACTATTACTACAAAACAGGAA28910.1152478806110091No Hit
CCAATCTCAAAGAACACGACTCTAGAAGTAAAATTCCAAAAAATAGAAAG28660.11425127147393709No Hit
GCCCACTTGGAGTTATATTATTTGACAAACGAGCAAAATACAGCGAAGAT28640.11417154274297131No Hit
CCAATCTCAAAGAACACGACTCTAGAAGTAAAATTCCAAGAAATAGAAAG28290.11277628995107047No Hit
GCACGTATAATTTCTCTTTTACATCAGCAGTAAAAGGATAACAAAAAAGA28170.1122979175652759No Hit
AATTGCTGTTCCCTCCTTGTTTCAAGGATAGTTCTTACGGCTTCGGATAA27850.1110222578698237No Hit
ACACATATTTAGCTTTATGTGAAATGTATCACACGAATTAGTGCTCCAAT27720.11050402111854626No Hit
CCCCCCTTTAATTGCTGTTCCCTCCTTGTTTCAAGGATAGTTCTTACGGC27120.1081121591895734No Hit
GTCCGTTTCAGTTCTGAGTTAACATTATTAGTATCAAGAGGGACGACGCC26910.10727500751443288No Hit
GTAGTATTCAAACGCGCAATGCTATATCTGTTTGCTTTGAACACTCTAAT26790.10679663512863832No Hit
CGAGGCAAAAAAGTCGTCTACAGTTTGTGCAATTCTTTTTTGTTGTTAAA26650.10623853401187798No Hit
CCGAGTGTAAGACCTATGTGAAGGAGTTCATTTGTATATATGTTACCATC25830.10296965604228174No Hit
CGTTACCCGTCATAGCCATGTTAGGCCAATACCCTAACATCAAAAGCTGA25240.10061765847879177No Hit
GTTGGTACGCTCATACAAGTATTAAGAGTATACAAAAAAAGGTTAATGAA25140.10021901482396296No Hit
CACGAAAAAGGTGATAGGATTATGAATGCATCCGTAGAGGAAGCACGTAT25120.10013928609299719No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)