FastQCFastQC Report
Thu 2 Nov 2023
HMLTGDMXY_n01_MHC-008.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHMLTGDMXY_n01_MHC-008.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences59138211
Sequences flagged as poor quality0
Sequence length101
%GC52

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CCCCGCCTCACCGGGTCAGTGAAAAAACGATCAGAGTAGTGGTATTTCAC3326380.5624755879071147No Hit
CCCCAGTCAAACTCCCCACCTGGCACTGTCCCCGGAGCGGGTCGCGGCCC1787300.3022242252137116No Hit
CTTCCTTGGATGTGGTAGCCGTTTCTCAGGCTCCCTCTCCGGAATCGAAC1309070.22135772757819813No Hit
CCGGGATTCGGCGAGTGCTGCTGCCGGGGGGGCTGTAACACTCGGGGGGG1146140.1938070125252859No Hit
GTCCACTCTCGACTGCCGGCGACGGCCGGGTATGGGCCCGACGCTCCAGC1121300.1896066825558859No Hit
CCCCGAGCCACCTTCCCCGCCGGGCCTTCCCAGCCGTCCCGGAGCCGGTC1100200.18603876941762745No Hit
CCCGCCTCACCGGGTCAGTGAAAAAACGATCAGAGTAGTGGTATTTCACC989690.1673520357252606No Hit
CCACTCTCGACTGCCGGCGACGGCCGGGTATGGGCCCGACGCTCCAGCGC931350.1574870095410901No Hit
CCCCGCCGGGCCTTCCCAGCCGTCCCGGAGCCGGTCGCGGCGCACCGCCG918770.15535978929088673No Hit
CTCCCGTCCACTCTCGACTGCCGGCGACGGCCGGGTATGGGCCCGACGCT886730.14994197237383458No Hit
CACCGGGTCAGTGAAAAAACGATCAGAGTAGTGGTATTTCACCGGCGGCC763300.12907052599206967No Hit
CCCCAGTCAAACTCCCCACCTGGCACTGTCCCCGGAGCGGGTCGCGGGCC741190.12533182649032112No Hit
CCCGGGGCTCCCGCCGGCTTCTCCGGGATCGGTCGCGTTACCGCACTGGA735110.12430372640119262No Hit
GGCAGACGTTCGAATGGGTCGTCGCCGCCACGGGGGGCGTGCGATCGGCC712380.12046018774561848No Hit
CTTGGATGTGGTAGCCGTTTCTCAGGCTCCCTCTCCGGAATCGAACCCTG705650.1193221756403825No Hit
CGGGATTCGGCGAGTGCTGCTGCCGGGGGGGCTGTAACACTCGGGGGGGG676730.11443193640064628No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)