FastQCFastQC Report
Thu 2 Nov 2023
HMLTGDMXY_n01_MHC-004.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHMLTGDMXY_n01_MHC-004.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences55910850
Sequences flagged as poor quality0
Sequence length101
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CCCGAGTGTAAAGCCTATGTAAAGGAGTTCATTTATATATATGTTACCAT5451060.9749556660290445No Hit
CTACAGTTTGTGCAATTCTTTTTTGTTGTTAAAGAACTATTGCCTAGCTC4026680.7201965271499181No Hit
CTCCTACACATATTTAGCTTTATGTGAAATGTATCACACGTATTAGTGCT3984460.7126452200243781No Hit
CTCCAATCTCAAAGAACACGACTCGGCATGAAAAATTCCAATGAATAAAT3844600.6876303973200193No Hit
GGCACCTTATCCAAGCGTTAGGAGCATCCCTCATCGCCAGTTCTGCTTAC3639810.6510024440694426No Hit
CCTACACATATTTAGCTTTATGTGAAATGTATCACACGTATTAGTGCTCC2939890.5258174397277094No Hit
CTCCAATTGTTACTCTGGGAAGGTTTTAAATTCCCACCATTCCAATTACA2769270.4953010015050746No Hit
CCCCAAGCTACTCCTATTAATCGTAACTAAGCCAAAATAAGAAAAAAGAA2511210.4491453805477828No Hit
CCCCTTTTAGTCTACAAGAGATTTCTGTTCTCTTTGAGCTTGTCTTTGGA2450820.43834425697337814No Hit
GTCGTCTACAGTTTGTGCAATTCTTTTTTGTTGTTAAAGAACTATTGCCT2363170.4226675144448707No Hit
CCCCGTTACCCGTCATAGCCATGTTAGGCCAATACCCTAACATCAAAAGC2307020.4126247409939216No Hit
CTTTTAGTCTACAAGAGATTTCTGTTCTCTTTGAGCTTGTCTTTGGACAT2293190.41015116028463167No Hit
CGCTCATACAAGTATTAAGAGTATAGCAAAGCAAAATGGAATCAGAAGAA2119370.37906238234618145No Hit
CCCCAGTCAAACTCCCCACCTGGCTATGTCTCCATAGAAATTGGGAGAAA1952680.34924884883703256No Hit
GCTCATACAAGTATTAAGAGTATAGCAAAGCAAAATGGAATCAGAAGAAA1867510.33401566958828205No Hit
GTACGCTCATACAAGTATTAAGAGTATAGCAAAGCAAAATGGAATCAGAA1756110.31409109323145684No Hit
GTCTCCTACACATATTTAGCTTTATGTGAAATGTATCACACGTATTAGTG1745540.31220058360765396No Hit
CTTAACTTTCGTTCTTGATTAATGGAAGTATTTTAGACAAATGCTTTCGC1681770.3007949262084193No Hit
GCCCTTTTTACCTATTGGGAGTTTGAGAATAGGTCAAGGAAAACGATTTC1639040.2931524024406712No Hit
GTCCACTCTCGACTGCCGGCGACGGCCGGGTATGGGCCCGACGCTCCAGC1594510.28518793758277683No Hit
TTTTAGTCTACAAGAGATTTCTGTTCTCTTTGAGCTTGTCTTTGGACATC1557030.2784844086612885No Hit
GCACCTTATCCAAGCGTTAGGAGCATCCCTCATCGCCAGTTCTGCTTACC1547570.276792429376409No Hit
CCCGTTACCCGTCATAGCCATGTTAGGCCAATACCCTAACATCAAAAGCT1491030.2666799020225949No Hit
CCGAGTGTAAAGCCTATGTAAAGGAGTTCATTTATATATATGTTACCATC1441810.2578766017687086No Hit
TACAGTTTGTGCAATTCTTTTTTGTTGTTAAAGAACTATTGCCTAGCTCA1431540.25603974899326337No Hit
CTCCCGTCCACTCTCGACTGCCGGCGACGGCCGGGTATGGGCCCGACGCT1424530.25478596730330516No Hit
CCCCGCCTCACCGGGTCAGTGAAAAAACGATCAGAGTAGTGGTATTTCAC1404840.25126428948942825No Hit
CGCCCTTTTTACCTATTGGGAGTTTGAGAATAGGTCAAGGAAAACGATTT1394020.24932906582532727No Hit
CCTGTGGTAACTTTTCAGACACCTCTATATGCACAGTAGTAAGTAATTTA1343960.2403755263960394No Hit
CACCATTCCAATTACAAAACCAAAAATTGGCCTTGCATTGTTATTTCTTG1334690.23871752978178654No Hit
CACCGGGTCAGTGAAAAAACGATCAGAGTAGTGGTATTTCACCGGCGGCC1287050.23019682226258412No Hit
CCACTCTCGACTGCCGGCGACGGCCGGGTATGGGCCCGACGCTCCAGCGC1283360.2295368430277844No Hit
CCGGAATCGAACTCTAATTCCCCGTTACCCGTCATAGCCATGTTAGGCCA1251030.2237544233364365No Hit
CCCATCACGATATATATTGATAAAGATTACCTACGCCTTTCGGCGGAGGA1196450.2139924540585593No Hit
CCCTTTTAGTCTACAAGAGATTTCTGTTCTCTTTGAGCTTGTCTTTGGAC1188910.21264387860316916No Hit
CCCTTGTCCGTTTCAGTTCTGAGTTAACATTATAGGTATCAAATTAAAGT1179080.2108857225386486No Hit
CTCCACTTCAGTCTTCAAAGTTCTCATTTGAATAGTTGCTACTTCCACCA1096710.19615334054123662No Hit
CTCGGGTCCTACCAAATATGCTCGTACTCAATAAAGCATAATTGCTTACC1033590.18486393964677697No Hit
TCCAATTGTTACTCTGGGAAGGTTTTAAATTCCCACCATTCCAATTACAA989400.17696028588368803No Hit
GTCCGTTTCAGTTCTGAGTTAACATTATAGGTATCAAATTAAAGTAATAA970580.17359421293004848No Hit
CCCGTCATAGCCATGTTAGGCCAATACCCTAACATCAAAAGCTGATAGGT957020.17116892338428052No Hit
CTTCCGTCAATTCTTTTAACTTTCTCGCTTGCGCGAATACTCGCCCCAGA919740.16450116569503057No Hit
GTAAAATTATATTAATCAACTCCAGCGATCCTGAGAGATCTTTCGAAAAT907630.1623352175829915No Hit
AGGCACCTTATCCAAGCGTTAGGAGCATCCCTCATCGCCAGTTCTGCTTA879970.15738805616441173No Hit
CCTTAACTTTCGTTCTTGATTAATGGAAGTATTTTAGACAAATGCTTTCG876280.15672807692961205No Hit
GGCAGACGTTCGAATGGGTCGTCGCCGCCACGGGGGGCGTGCGATCGGCC866040.15489658983900262No Hit
GCCGTTCCCTCCTTTTTTCAAGGATAGTCCTTACCACTTCGGATAATTCA853880.15272169891890394No Hit
CTCCCTTAACTTTCGTTCTTGATTAATGGAAGTATTTTAGACAAATGCTT849350.1519114805087027No Hit
CTCGGCATGAAAAATTCCAATGAATAAATTAAAACGAACTATGGGCCTTT841020.1504216086859706No Hit
CTCAGAGCCAATCCTTTTCCCGAAGTTACGGATCTATTTTGCCGACTTCC838830.15002991369295943No Hit
CCCATCACGATATATATTGATAAAGATTACCTACACCTTTCGGCGGAGGA826070.14774770907614532No Hit
CCACGCTTTCACGTTTTCTACTTGTTCTGAAAATAGAAATCAAACAAGCT816490.14603426705192282No Hit
GTCTACAGTTTGTGCAATTCTTTTTTGTTGTTAAAGAACTATTGCCTAGC806250.14420277996131342No Hit
CTCCTTTTTTCAAGGATAGTCCTTACCACTTCGGATAATTCATAAAAACG804730.14393091859630108No Hit
CCGTTACCCGTCATAGCCATGTTAGGCCAATACCCTAACATCAAAAGCTG802830.14359109189003566No Hit
CCAATTGTTACTCTGGGAAGGTTTTAAATTCCCACCATTCCAATTACAAA793150.14185976424969393No Hit
CCCTTACCTACTTTTTTCTATTTAACCAGAGGCTGTTAACCTTGGAGACC792910.14181683877100779No Hit
CACATATTTAGCTTTATGTGAAATGTATCACACGTATTAGTGCTCCAATC752560.13459999266689737No Hit
CGAGTGTAAAGCCTATGTAAAGGAGTTCATTTATATATATGTTACCATCT723100.12933089015817145No Hit
GCCACGCTTTCACGTTTTCTACTTGTTCTGAAAATAGAAATCAAACAAGC720350.12883903571489252No Hit
CCCTGTGGTAACTTTTCAGACACCTCTATATGCACAGTAGTAAGTAATTT717180.1282720616839129No Hit
GTCCCCAAGCTACTCCTATTAATCGTAACTAAGCCAAAATAAGAAAAAAG701350.12544076865223833No Hit
GTACAAAGGGCAGGGACGTAGTCAGCACAATCTGATGAATCATGCTTACT693730.1240778847039528No Hit
CCTCACGGTACTTGTTTGCTATCGGTCTCCTACACATATTTAGCTTTATG686600.12280264027465153No Hit
CTCGACTGCCGGCGACGGCCGGGTATGGGCCCGACGCTCCAGCGCCATCC681670.12192087939997334No Hit
CATCACGATATATATTGATAAAGATTACCTACGCCTTTCGGCGGAGGAAA678050.12127342009645711No Hit
CTTGTCCGTTTCAGTTCTGAGTTAACATTATAGGTATCAAATTAAAGTAA661670.11834375617612682No Hit
CCTCGTTCAAGATTAATAATTGCAATAATCTATCCCCATCACGATATATA654240.11701485489846783No Hit
GCCCTTCCGTCAATTCTTTTAACTTTCTCGCTTGCGCGAATACTCGCCCC633130.11323920133569781No Hit
CCGACATCGAAGGATCAAAAAGCTACGTCGCTAGGAGCGCTTGGCAGCCA630470.11276344394692622No Hit
CCCGGGGCTCCCGCCGGCTTCTCCGGGATCGGTCGCGTTACCGCACTGGA626600.11207127060311192No Hit
CCCCGTTCAAGAAATTTTTTGAATTGAGTTCAACGAACCTCTTCTCCAAG625830.11193355135899383No Hit
CCTTCCGTCAATTCTTTTAACTTTCTCGCTTGCGCGAATACTCGCCCCAG624050.11161518739207148No Hit
GTCCTACCAAATATGCTCGTACTCAATAAAGCATAATTGCTTACCGGTAT620350.11095341959565988No Hit
GCTGGATAGTAGGTAGGGACAGTGGGAATCTCGTTCATCCATTCATGCGC617850.11050627919267907No Hit
CCGGCGACGGCCGGGTATGGGCCCGACGCTCCAGCGCCATCCATTTTCAG614390.1098874368749536No Hit
CCAATCTCAAAGAACACGACTCGGCATGAAAAATTCCAATGAATAAATTA613580.10974256338438781No Hit
CCAGAACCCAAAGACTTTGATTTCTCATAAGGTACTGAAGGAAGCAATCT598480.1070418353503837No Hit
CCCACTTGGAGTTATTTTATTTAATATGAGTGAAATACAAATTGAGTTAT591230.10574512818173933No Hit
CTACACATATTTAGCTTTATGTGAAATGTATCACACGTATTAGTGCTCCA589230.10538741585935467No Hit
CTTCCTTGGATGTGGTAGCCGTTTCTCAGGCTCCCTCTCCGGAATCGAAC584220.1044913464917811No Hit
CTCTCGGGTCCTACCAAATATGCTCGTACTCAATAAAGCATAATTGCTTA582730.10422485081160456No Hit
CCCCTCTTTACTTGTTTTCTCTCTTAATCCGTTCCATCTTAACGATTTCA571110.10214654221854971No Hit
GCTACTTCCACCAAGATCTGCACTAGGGAGATGTTCCACGAAAACTCACG569700.10189435503126852No Hit
CCGGATTTTCAAGGGCCAGCGAGAGCTCACCGGACGCCGCCGGAACCGCG568620.10170119037718083No Hit
CAGCGATCCTGAGAGATCTTTCGAAAATAACCAGCTAATAGGCTTTTCGA566370.10129876401449808No Hit
CTCGGGTCCTACCAAATATGCTCGTACTCAATAAAGCATAATTTTGCTTA564260.10092137751438228No Hit
CCACGATTTTCTACTCATAAAAAGATTCTAAAAAAAAAAATAAATTTTTT563530.10079081251671188No Hit
CGTCTACAGTTTGTGCAATTCTTTTTTGTTGTTAAAGAACTATTGCCTAG561910.1005010655355803No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)