FastQCFastQC Report
Thu 2 Nov 2023
HMLTGDMXY_n01_MHC-003.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHMLTGDMXY_n01_MHC-003.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences44092252
Sequences flagged as poor quality0
Sequence length101
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CCCGAGTGTAAAGCCTATGTAAAGGAGTTCATTTATATATATGTTACCAT3459370.7845754850534739No Hit
CTCCTACACATATTTAGCTTTATGTGAAATGTATCACACGTATTAGTGCT2483750.563307585196601No Hit
CCTACACATATTTAGCTTTATGTGAAATGTATCACACGTATTAGTGCTCC2233520.5065561178412933No Hit
CTCCAATCTCAAAGAACACGACTCGGCATGAAAAATTCCAATGAATAAAT2017300.4575180238015513No Hit
CTACAGTTTGTGCAATTCTTTTTTGTTGTTAAAGAACTATTGCCTAGCTC1767840.40094119030255015No Hit
CGCTCATACAAGTATTAAGAGTATAGCAAAGCAAAATGGAATCAGAAGAA1573180.35679284423939156No Hit
GCTCATACAAGTATTAAGAGTATAGCAAAGCAAAATGGAATCAGAAGAAA1533320.347752707210328No Hit
CCCCGTTACCCGTCATAGCCATGTTAGGCCAATACCCTAACATCAAAAGC1480810.33584358539908554No Hit
CCCCAAGCTACTCCTATTAATCGTAACTAAGCCAAAATAAGAAAAAAGAA1349610.306087790662178No Hit
GTACGCTCATACAAGTATTAAGAGTATAGCAAAGCAAAATGGAATCAGAA1273320.28878543105487103No Hit
GGCACCTTATCCAAGCGTTAGGAGCATCCCTCATCGCCAGTTCTGCTTAC1221320.27699197582377966No Hit
CTACAGTTTGTGCAATTCTTTTTTGTTGTTAAAGAACTATTTGCCTAGCT1122340.25454358738582916No Hit
CCGAGTGTAAAGCCTATGTAAAGGAGTTCATTTATATATATGTTACCATC1002970.22747080371399495No Hit
CCCGTTACCCGTCATAGCCATGTTAGGCCAATACCCTAACATCAAAAGCT989490.22441357724255045No Hit
CCCCTTTTAGTCTACAAGAGATTTCTGTTCTCTTTGAGCTTGTCTTTGGA967200.21935826729829996No Hit
GCCCTTTTTACCTATTGGGAGTTTGAGAATAGGTCAAGGAAAACGATTTC923430.20943135315474476No Hit
CCCCAGTCAAACTCCCCACCTGGCTATGTCTCCATAGAAATTGGGAGAAA904540.2051471537448348No Hit
GCCGTTCCCTCCTTTTTTCAAGGATAGTCCTTACCACTTCGGATAATTCA893730.2026954758400637No Hit
CTCGGGTCCTACCAAATATGCTCGTACTCAATAAAGCATAATTGCTTACC846930.19208136613208143No Hit
GTCTCCTACACATATTTAGCTTTATGTGAAATGTATCACACGTATTAGTG842780.19114015768575396No Hit
GTCGTCTACAGTTTGTGCAATTCTTTTTTGTTGTTAAAGAACTATTGCCT833320.18899465602255924No Hit
CTTTTAGTCTACAAGAGATTTCTGTTCTCTTTGAGCTTGTCTTTGGACAT827910.18776768308409378No Hit
CCGGAATCGAACTCTAATTCCCCGTTACCCGTCATAGCCATGTTAGGCCA759990.17236361617456056No Hit
CACATATTTAGCTTTATGTGAAATGTATCACACGTATTAGTGCTCCAATC741870.16825405062095716No Hit
CCCCGTTCAAGAAATTTTTTGAATTGAGTTCAACGAACCTCTTCTCCAAG734310.16653946366812927No Hit
CGCCCTTTTTACCTATTGGGAGTTTGAGAATAGGTCAAGGAAAACGATTT703720.15960173683122375No Hit
CCTGTGGTAACTTTTCAGACACCTCTATATGCACAGTAGTAAGTAATTTA670560.15208114114924318No Hit
CCCACTTGGAGTTATTTTATTTAATATGAGTGAAATACAAATTGAGTTAT670340.15203124576172702No Hit
TTTTAGTCTACAAGAGATTTCTGTTCTCTTTGAGCTTGTCTTTGGACATC628320.14250122674614124No Hit
CCACGCTTTCACGTTTTCTACTTGTTCTGAAAATAGAAATCAAACAAGCT609280.13818300775383394No Hit
TACAGTTTGTGCAATTCTTTTTTGTTGTTAAAGAACTATTGCCTAGCTCA583840.13241328657923845No Hit
CGAGTGTAAAGCCTATGTAAAGGAGTTCATTTATATATATGTTACCATCT573680.13010902686485598No Hit
GCACCTTATCCAAGCGTTAGGAGCATCCCTCATCGCCAGTTCTGCTTACC561900.1274373556605818No Hit
GTCGTCTACAGTTTGTGCAATTCTTTTTTGTTGTTAAAGAACTATTTGCC558230.12660500987792594No Hit
CCCCGCCTCACCGGGTCAGTGAAAAAACGATCAGAGTAGTGGTATTTCAC538020.12202143814291908No Hit
GTCAGTATCAACTCGATCCTCCATCAGACTTTCGTCTGACTTCAATCTCA524060.118855349007803No Hit
CCTCGTTCAAGATTAATAATTGCAATAATCTATCCCCATCACGATATATA514580.11670531140028864No Hit
CTCTCGGGTCCTACCAAATATGCTCGTACTCAATAAAGCATAATTGCTTA505720.11469588806668346No Hit
CCGTTACCCGTCATAGCCATGTTAGGCCAATACCCTAACATCAAAAGCTG501370.11370932017715947No Hit
GCCACGCTTTCACGTTTTCTACTTGTTCTGAAAATAGAAATCAAACAAGC490890.1113324853536626No Hit
CATGGTTTCTGGCTTAAGAGGCGTTCAGCCATTATCCAACAGACTTAGCT485100.11001932947312376No Hit
CTACACATATTTAGCTTTATGTGAAATGTATCACACGTATTAGTGCTCCA484910.10997623800208707No Hit
AAGAAACTCATAATACTATTGGCACAGGAATATCAACCTGTTTCCCTTTT483130.10957253895763819No Hit
GCCCACTTGGAGTTATTTTATTTAATATGAGTGAAATACAAATTGAGTTA472070.10706416174887143No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)