FastQCFastQC Report
Thu 2 Nov 2023
HMLTGDMXY_n01_KHC-003.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHMLTGDMXY_n01_KHC-003.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2475101
Sequences flagged as poor quality0
Sequence length101
%GC41

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CCTGAATTGATTCAGCGAACCTCTTCTCCAAGTAAATTGATTCCAAGGTT417811.6880523259454865No Hit
CCCCAAGCTACTCCTATTAATCGTAACTAAGCCAAAATAAGAAAAAAGAA368151.4874140489620424No Hit
CCTACACATATTTAGCTTTATGTGAAATGTATCACACGAATTAGTGCTCC330861.336753530461989No Hit
CTACAGTTTGTGCAATTCTTTTTTGTTGTTAAAGAACTATTGCCTAGCTC319061.2890787083032167No Hit
CTCCAATCTCAAAGAACACGACTCTAGAAGTAAAATTCCAAAAAATAGAA292591.1821335775792583No Hit
CTCCTACACATATTTAGCTTTATGTGAAATGTATCACACGAATTAGTGCT270881.094419985285449No Hit
CTTTAATTGCTGTTCCCTCCTTGTTTCAAGGATAGTTCTTACGGCTTCGG236830.9568498416832282No Hit
CCCGAGGCAAAAAAGTCGTCTACAGTTTGTGCAATTCTTTTTTGTTGTTA189710.7664737721814181No Hit
CTCGGGTCCTACCAAATATGCTCTTACTCAATATAGCGTAATTGCTTACC177360.7165768184813468No Hit
CCCCGTTACCCGTCATAGCCATGTTAGGCCAATACCCTAACATCAAAAGC142040.5738755711383091No Hit
CGCTCATACAAGTATTAAGAGTATACAAAAAAAGGTTAATGAAATAATTA140730.5685828578308522No Hit
GTCGTCTACAGTTTGTGCAATTCTTTTTTGTTGTTAAAGAACTATTGCCT136500.551492646158682No Hit
GTACGCTCATACAAGTATTAAGAGTATACAAAAAAAGGTTAATGAAATAA134890.5449878611014257No Hit
CTGAATTGATTCAGCGAACCTCTTCTCCAAGTAAATTGATTCCAAGGTTT130400.5268471872460962No Hit
CCCCGAGGCAAAAAAGTCGTCTACAGTTTGTGCAATTCTTTTTTGTTGTT125780.508181282299187No Hit
CACATATTTAGCTTTATGTGAAATGTATCACACGAATTAGTGCTCCAATC120020.4849095047030404No Hit
GCACCATGTAGGAACAACTCAAACAGCAAAGAAATAAATCAATGCAATTT104430.4219221761051367No Hit
CCCGTTACCCGTCATAGCCATGTTAGGCCAATACCCTAACATCAAAAGCT97350.3933172828098732No Hit
GCTCATACAAGTATTAAGAGTATACAAAAAAAGGTTAATGAAATAATTAA95190.38459036621131826No Hit
TACAGTTTGTGCAATTCTTTTTTGTTGTTAAAGAACTATTGCCTAGCTCA95070.38410553751139853No Hit
CCCGAGTGTAAGACCTATGTGAAGGAGTTCATTTGTATATATGTTACCAT93660.3784088002873418No Hit
CCCCCAAGCTACTCCTATTAATCGTAACTAAGCCAAAATAAGAAAAAAGA92940.3754998280878235No Hit
CTCTCGGGTCCTACCAAATATGCTCTTACTCAATATAGCGTAATTGCTTA89090.35994490729873246No Hit
CTACACATATTTAGCTTTATGTGAAATGTATCACACGAATTAGTGCTCCA87140.35206644092503697No Hit
GTCTCCTACACATATTTAGCTTTATGTGAAATGTATCACACGAATTAGTG82500.3333197311948078No Hit
CCCGAGTGTAAGACCTATGTGAAGGAGTTCATTTGTATGTATGTTACCAT82380.3328349024948881No Hit
AATTGATTCAGCGAACCTCTTCTCCAAGTAAATTGATTCCAAGGTTTGCT80180.32394637632969325No Hit
CCCCCTTTAATTGCTGTTCCCTCCTTGTTTCAAGGATAGTTCTTACGGCT78870.3186536630222363No Hit
CCATGCTGTAGTATTCAAACGCGCAATGCTATATCTGTTTGCTTTGAACA76770.31016916077364115No Hit
CCCCTTTAATTGCTGTTCCCTCCTTGTTTCAAGGATAGTTCTTACGGCTT69270.27986736702865866No Hit
GAATTGATTCAGCGAACCTCTTCTCCAAGTAAATTGATTCCAAGGTTTGC65790.2658073347309867No Hit
GTTAAGAAAGCACCATGTAGGAACAACTCAAACAGCAAAGAAATAAATCA59890.24196992365160047No Hit
CCGAGGCAAAAAAGTCGTCTACAGTTTGTGCAATTCTTTTTTGTTGTTAA59160.23902054906042217No Hit
GTCACGCTTAGTTCAAAGCCTCTCGGGTCCTACCAAATATGCTCTTACTC58270.23542473620268423No Hit
CCTTTAATTGCTGTTCCCTCCTTGTTTCAAGGATAGTTCTTACGGCTTCG58210.2351823218527244No Hit
GTCTACAGTTTGTGCAATTCTTTTTTGTTGTTAAAGAACTATTGCCTAGC53120.21461750449779626No Hit
CCCTTTAATTGCTGTTCCCTCCTTGTTTCAAGGATAGTTCTTACGGCTTC52460.21195094664823777No Hit
CCCAAGCTACTCCTATTAATCGTAACTAAGCCAAAATAAGAAAAAAGAAC50290.20318362765802284No Hit
CTTAAACTTCCTTGTGTTAGACACACATCGTTCCTCTAAGAAGCTTTACA49650.20059787459178435No Hit
GTTCCCTCCTTGTTTCAAGGATAGTTCTTACGGCTTCGGATAATTCATAA49600.20039586263348444No Hit
CCCGTCATAGCCATGTTAGGCCAATACCCTAACATCAAAAGCTGATAGGT49470.19987063154190474No Hit
CACGAATTAGTGCTCCAATCTCAAAGAACACGACTCTAGAAGTAAAATTC48370.1954263684593073No Hit
GGTTAAGAAAGCACCATGTAGGAACAACTCAAACAGCAAAGAAATAAATC46870.1893660097103108No Hit
CCAATCTCAAAGAACACGACTCTAGAAGTAAAATTCCAAAAAATAGAAAG46770.18896198579371104No Hit
GGGTTAAGAAAGCACCATGTAGGAACAACTCAAACAGCAAAGAAATAAAT45350.18322484617799437No Hit
CCCCTTTTAGTCTACAAGAGATTTCTGTTCTCTTTGAGCTTGTCTTTGGA44240.17874018070373693No Hit
GTGCAATTCTTTTTTGTTGTTAAAGAACTATTGCCTAGCTCACAGCTCAA41740.16863958278874278No Hit
TGAATTGATTCAGCGAACCTCTTCTCCAAGTAAATTGATTCCAAGGTTTG41240.16661946320574395No Hit
TTTAATTGCTGTTCCCTCCTTGTTTCAAGGATAGTTCTTACGGCTTCGGA40820.1649225627560249No Hit
GTGGGTTAAGAAAGCACCATGTAGGAACAACTCAAACAGCAAAGAAATAA40340.16298324795634603No Hit
CCGTTACCCGTCATAGCCATGTTAGGCCAATACCCTAACATCAAAAGCTG39870.1610843355483271No Hit
GTTGCCTTAAACTTCCTTGTGTTAGACACACATCGTTCCTCTAAGAAGCT39700.16039749489010752No Hit
CTCGTCACGCTTAGTTCAAAGCCTCTCGGGTCCTACCAAATATGCTCTTA39460.15942783749026807No Hit
CCCCAGTCAAACTCCCCACCTGGCTATGTCTCCATAGAGTCGGGAGGAAA39360.1590238135736683No Hit
GTAGTATTCAAACGCGCAATGCTATATCTGTTTGCTTTGAACACTCTAAT39250.15857938726540854No Hit
CCAAGCTACTCCTATTAATCGTAACTAAGCCAAAATAAGAAAAAAGAACA38220.15441794092443095No Hit
CTCCTTGTTTCAAGGATAGTTCTTACGGCTTCGGATAATTCATAAAAACG38140.15409472179115116No Hit
CGGATAATTCATAAAAACGAATTACTATTACTACAAAACAGGAAATTAAG37730.1524382237330921No Hit
GCTTCGGATAATTCATAAAAACGAATTACTATTACTACAAAACAGGAAAT37110.14993327545017354No Hit
CTCCTATTAATCGTAACTAAGCCAAAATAAGAAAAAAGAACAAAATTCTG36860.1489232156586741No Hit
CTTCGGATAATTCATAAAAACGAATTACTATTACTACAAAACAGGAAATT36410.14710510803397517No Hit
GTCAGTATCAATTCGATCTTCCATCAGACTTTCGTCTGACTTCAATCTCG36010.1454890123675761No Hit
TAAGAAAGCACCATGTAGGAACAACTCAAACAGCAAAGAAATAAATCAAT35570.14371130713453714No Hit
TCCTACACATATTTAGCTTTATGTGAAATGTATCACACGAATTAGTGCTC35440.14318607604295744No Hit
GGATAATTCATAAAAACGAATTACTATTACTACAAAACAGGAAATTAAGA35090.14177199233485824No Hit
CATGGTTTCTGGCTTAAGAGGCGTTCAGCCATTATCCAACAGACTTAGCT33760.13639847424408136No Hit
TCTACAGTTTGTGCAATTCTTTTTTGTTGTTAAAGAACTATTGCCTAGCT33300.1345399642277224No Hit
GCTCCAATCTCAAAGAACACGACTCTAGAAGTAAAATTCCAAAAAATAGA32860.13276225899468344No Hit
CCTCCTTGTTTCAAGGATAGTTCTTACGGCTTCGGATAATTCATAAAAAC32780.13243903986140362No Hit
GTCCTACCAAATATGCTCTTACTCAATATAGCGTAATTGCTTACCGGTAT32460.13114616332828438No Hit
CTTTTTTGTTGTTAAAGAACTATTGCCTAGCTCACAGCTCAAATAGTCCA32380.13082294419500456No Hit
CCCTATTACACTCAAAATACTATCGGCACAGGAATATCAACCTGTTTCCC32290.13045932267006477No Hit
ACACATATTTAGCTTTATGTGAAATGTATCACACGAATTAGTGCTCCAAT32230.1302169083201049No Hit
GTTGGTACGCTCATACAAGTATTAAGAGTATACAAAAAAAGGTTAATGAA31850.1286816174370258No Hit
CTCTATTACACTCAAAATACTATCGGCACAGGAATATCAACCTGTTTCCC31470.1271463265539467No Hit
TACACATATTTAGCTTTATGTGAAATGTATCACACGAATTAGTGCTCCAA30420.12290407542964914No Hit
CGTCTACAGTTTGTGCAATTCTTTTTTGTTGTTAAAGAACTATTGCCTAG30010.1212475773715901No Hit
CTTTTAGTCTACAAGAGATTTCTGTTCTCTTTGAGCTTGTCTTTGGACAT29990.12116677258827015No Hit
ATTGATTCAGCGAACCTCTTCTCCAAGTAAATTGATTCCAAGGTTTGCTA29670.11987389605515088No Hit
CAAAAAAATAATTTGCCTGAATTGATTCAGCGAACCTCTTCTCCAAGTAA28650.11575285210583326No Hit
CCTATGTGAAGGAGTTCATTTGTATATATGTTACCATCCCTATTACACTC28330.11445997557271399No Hit
GTTTGTGCAATTCTTTTTTGTTGTTAAAGAACTATTGCCTAGCTCACAGC27940.11288428229797491No Hit
TCCAATCTCAAAGAACACGACTCTAGAAGTAAAATTCCAAAAAATAGAAA27530.11122778423991588No Hit
GCTGTTCCCTCCTTGTTTCAAGGATAGTTCTTACGGCTTCGGATAATTCA27200.10989450531513664No Hit
CACGTCAGTATCAATTCGATCTTCCATCAGACTTTCGTCTGACTTCAATC26710.10791478812379778No Hit
CGAGGCAAAAAAGTCGTCTACAGTTTGTGCAATTCTTTTTTGTTGTTAAA26230.10597547332411889No Hit
GGATTATGAATGCATCCGTAGAGGAAGCACGTACAATTTCTCTTTTACAT25580.10334931786622041No Hit
CGCTGATGTTCCTTTTAATAAAGAAGAAAAATAAATCCGAAGAATCATTT25400.10262207481634082No Hit
CCCACTTGGAGTTATATTATTTGACAAACGAGCAAAATACAGCGAAGATA25110.1014504054582015No Hit
AATTGCTGTTCCCTCCTTGTTTCAAGGATAGTTCTTACGGCTTCGGATAA25040.10116758871658166No Hit
CCCATCACGATATGTACTGATAAAGATTACCTACACTTTTCAGTGGAGGA24910.10064235762500197No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)