FastQCFastQC Report
Mon 19 Jun 2017
HMLJNBGX2_n01_tga1_input_60.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHMLJNBGX2_n01_tga1_input_60.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences26738120
Sequences flagged as poor quality0
Sequence length75
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TATGCCG34850.046.528846
ATGCCGT35000.046.3293947
CGTATGC36000.045.42317244
TGCCGTC35900.045.2640348
GTATGCC37000.044.01142545
CACGGCT38950.041.36259531
GCACACG41600.039.30895611
ACGTCTG44600.036.51001415
CACACGT46000.035.7738912
ACACGTC47700.034.4986113
GCTACAT49750.032.38337735
CGTCTGA50950.032.02734816
CATCTCG52400.030.87734239
TCTCGTA52850.030.61437641
CTACATC53950.030.05428336
ATCTCGT57750.028.19595140
AGCACAC59050.027.80962810
GAGCACA60700.027.2814859
CTGAACT66100.025.05184619
AGAGCAC66350.024.8062938
Produced by FastQC (version 0.11.2)