FastQCFastQC Report
Mon 19 Jun 2017
HMLJNBGX2_n01_tga1_input_5.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHMLJNBGX2_n01_tga1_input_5.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences31459411
Sequences flagged as poor quality0
Sequence length75
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACGATCAGATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAGG467160.1484961050287941TruSeq Adapter, Index 9 (100% over 63bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TATGCCG59900.059.32477646
GTATGCC61550.057.6779145
CGTATGC61800.057.27364744
ATGCCGT63000.056.62466447
TGCCGTC65600.054.43307548
GCACACG69050.051.96065511
ACGTCTG70150.051.1941615
CACACGT71100.050.36480712
ACACGTC72250.049.6105113
CGTCTGA73600.048.7473316
TCTCGTA71850.048.35032341
CGATCAG75650.046.5601333
AGCACAC79950.045.2223710
ATCTCGT77150.044.84992240
GAGCACA82900.043.4889039
CTGAACT83550.042.81798619
TCTGAAC83600.042.5860518
AGAGCAC87900.040.8648228
TGCTTGA89950.039.84917458
AGATCTC87900.039.48285338
Produced by FastQC (version 0.11.2)