FastQCFastQC Report
Mon 19 Jun 2017
HMLJNBGX2_n01_tga1_input_30.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHMLJNBGX2_n01_tga1_input_30.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences31443445
Sequences flagged as poor quality0
Sequence length75
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACTAGCTTATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAGG351030.11163853070170905TruSeq Adapter, Index 10 (100% over 63bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTATGC53550.051.7315144
ATGCCGT53150.051.66935747
GTATGCC54650.050.75555845
TGCCGTC54700.050.39444748
TATGCCG54900.050.21078546
GCACACG58900.046.97511311
ACGTCTG63050.043.77234615
CACACGT65400.042.41135812
ACACGTC67900.040.79817213
CACTAGC68750.040.14373431
CGTCTGA71800.038.7263216
GTCACTA75100.036.93322829
TCTCGTA76450.036.1004241
GAGCACA80700.034.92749
AGTCACT81300.034.2439728
ATCTCGT82250.033.6805740
AGCACAC83050.033.56473510
ACTAGCT83100.033.5021732
GCTTATC83200.033.1302436
TCGGAAG89200.031.4158733
Produced by FastQC (version 0.11.2)