FastQCFastQC Report
Mon 19 Jun 2017
HMLJNBGX2_n01_tga1_input_300.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHMLJNBGX2_n01_tga1_input_300.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences30218809
Sequences flagged as poor quality0
Sequence length75
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACCTTGTAATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAGG398060.13172590620629687TruSeq Adapter, Index 12 (100% over 63bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TATGCCG50950.056.0003946
CGTATGC51750.055.1310444
ATGCCGT52800.053.9729147
TGCCGTC52800.053.77688648
GTATGCC53300.053.53097545
GCACACG56150.053.0233411
CACACGT59350.050.16420412
ACGTCTG59450.050.02121715
AGTCACC59700.049.522928
ACACGTC60750.049.06429713
CGTCTGA61700.048.42067316
TCTCGTA61650.045.326541
AGCACAC68950.043.53030410
GAGCACA69800.042.951079
AATCTCG67150.041.511339
CTGAACT71950.041.47437319
ATCTCGT67850.041.1338140
AGAGCAC72850.040.922438
TCTGAAC74050.040.67089518
TCGGAAG73750.040.382733
Produced by FastQC (version 0.11.2)