FastQCFastQC Report
Mon 19 Jun 2017
HMLJNBGX2_n01_tga1_input_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHMLJNBGX2_n01_tga1_input_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences33463486
Sequences flagged as poor quality0
Sequence length75
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TGCCGTC44550.043.83235548
ATGCCGT45150.043.47910347
CGTATGC46450.042.2598544
TATGCCG46750.041.7697246
GTATGCC47500.041.54580345
GCACACG51600.039.31276311
ACGTCTG53400.037.79300715
CACACGT56800.035.46996312
GTCACAC57850.034.5277429
ACACGTC59500.034.03403513
CGTCTGA62650.032.8187516
GAGCACA75700.027.0712439
TCTCGTA74050.026.60164641
AGCACAC78950.026.0001210
CACACTT78050.025.9451931
AATCTCG76650.025.654339
ATCTCGT76550.025.46248640
TCACACT81250.024.88092630
TCGGAAG84000.024.4455643
AGAGCAC85700.024.1981438
Produced by FastQC (version 0.11.2)