FastQCFastQC Report
Mon 19 Jun 2017
HMLJNBGX2_n01_tga1_input_0.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHMLJNBGX2_n01_tga1_input_0.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences28209778
Sequences flagged as poor quality0
Sequence length75
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TATGCCG28850.051.78093346
GTATGCC29900.050.19322245
TGCCGTC29950.049.8791348
CGTATGC30250.049.8377544
ATGCCGT30600.048.70685647
GCACACG35300.043.19763611
CACACGT37700.040.6302412
ACGTCTG37750.040.48460815
ACACGTC39450.038.9147113
CGTCTGA39950.038.3413216
AGTCACC39150.038.24333628
CATCTCG39800.037.79224439
TCTCGTA40800.036.61210641
AGCACAC45200.034.49958810
ATCTCGT45400.033.20646340
GAGCACA46600.032.8711439
CACCAGA45400.032.5983331
CCAGATC48450.031.04480433
CTGAACT50100.030.29775219
ACCAGAT49850.030.17282732
Produced by FastQC (version 0.11.2)